LOCUS       sequence-263752-        9042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid containing nopaline synthase promoter and TEV 
            enhancer to drive bialophos resistance gene (bar) for plant 
            transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Testroet A, Lee K, Luth D, Wang K
  TITLE     Comparison of transformation frequency using the bar gene regulated 
            by the CaMV 35S or NOS promoter in Agrobacterium-mediated soybean 
            (Glycine max L.) transformation
  JOURNAL   In Vitro Cell.Dev.Biol.-Plant (2017) 53: 188.
  PUBMED    16249869
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "In Vitro 
            Cell.Dev.Biol.-Plant (2017) 53: 188."
COMMENT     Sequence Label: pTF101.2
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1333..1962
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2391..3464
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3530..3724
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3960..3982)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4068..4208
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4123..4142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4223..4240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4394..4982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4474..4493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5228..6019)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6540..6564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6764..6786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6778..6795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6779..6795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7365..7913)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(8105..8284)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    8683..8739
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8748..8764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8748..8764)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8761..8783)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8772..8788
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8796..8802,8803..8820,8821..8826))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8841..8862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(9020..9042,1..2)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
       61 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
      121 ctttaaagta ctttaaagta ctttaaagta ctttgatcca acccctccgc tgctatagtg
      181 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
      241 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
      301 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
      361 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
      421 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
      481 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
      541 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
      601 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
      661 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
      721 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
      781 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
      841 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
      901 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
      961 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     1021 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     1081 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     1141 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     1201 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     1261 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     1321 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     1381 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     1441 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     1501 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     1561 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     1621 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     1681 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     1741 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     1801 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     1861 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     1921 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     1981 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     2041 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     2101 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     2161 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     2221 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     2281 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     2341 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     2401 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     2461 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     2521 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     2581 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     2641 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     2701 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     2761 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     2821 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     2881 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     2941 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     3001 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     3061 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     3121 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     3181 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     3241 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     3301 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     3361 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     3421 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     3481 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     3541 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     3601 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     3661 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     3721 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     3781 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     3841 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     3901 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3961 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4021 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     4081 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     4141 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4201 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4261 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4321 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4381 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4441 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4501 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4561 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4621 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4681 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4741 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4801 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     4861 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4921 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4981 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5041 aagggatttt ggtcatgcat gatatatctc ccaatttgtg tagggcttat tatgcacgct
     5101 taaaaataat aaaagcagac ttgacctgat agtttggctg tgagcaatta tgtgcttagt
     5161 gcatctaacg cttgagttaa gccgcgccgc gaagcggcgt cggcttgaac gaatttctag
     5221 ctagacatta tttgccgact accttggtga tctcgccttt cacgtagtgg acaaattctt
     5281 ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa gcctgtctag
     5341 cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag tcggcagcga
     5401 catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac aacgtaagca
     5461 ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt aaggtttcat
     5521 ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc gccgctggac
     5581 ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga tcaatgtcga
     5641 tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct ccaaattgca
     5701 gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca atggtgactt
     5761 ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa aggtcgttga
     5821 tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc aaatcaatat
     5881 cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg
     5941 tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg
     6001 cgatcaccgc ttcccccatg atgtttaact ttgttttagg gcgactgccc tgctgcgtaa
     6061 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     6121 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
     6181 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
     6241 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgcccgaa
     6301 ttgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat
     6361 catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg
     6421 agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg
     6481 cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt
     6541 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
     6601 acgtttttaa tgtactgaat taacgccgaa ttgctctagc attcgccatt caggctgcgc
     6661 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6721 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6781 aaaacgacgg ccagtgccaa gctaattcgc ttcaagacgt gctcaaatca ctatttccac
     6841 acccctatat ttctattgca ctccctttta actgtttttt attacaaaaa tgccctggaa
     6901 aatgcactcc ctttttgtgt ttgttttttt gtgaaacgat gttgtcaggt aatttatttg
     6961 tcagtctact atggtggccc attatattaa tagcaactgt cggtccaata gacgacgtcg
     7021 attttctgca tttgtttaac cacgtggatt ttatgacatt ttatattagt taatttgtaa
     7081 aacctaccca attaaagacc tcatatgttc taaagactaa tacttaatga taacaatttt
     7141 cttttagtga agaaagggat aattagtaaa tatggaacaa gggcagaaga tttattaaag
     7201 ccgcgtaaga gacaacaagt aggtacgtgg agtgtcttag gtgacttacc cacataacat
     7261 aaagtgacat taacaaacat agctaatgct cctatttgaa tagtgcatat cagcatacct
     7321 tattacatat agataggagc aaactctagc tagattgttg agcagatctc ggtgacgggc
     7381 aggaccggac ggggcggtac cggcaggctg aagtccagct gccagaaacc cacgtcatgc
     7441 cagttcccgt gcttgaagcc ggccgcccgc agcatgccgc ggggggcata tccgagcgcc
     7501 tcgtgcatgc gcacgctcgg gtcgttgggc agcccgatga cagcgaccac gctcttgaag
     7561 ccctgtgcct ccagggactt cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc
     7621 tggtggcggg gggagacgta cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc
     7681 ttccaggggc ccgcgtaggc gatgccggcg acctcgccgt ccacctcggc gacgagccag
     7741 ggatagcgct cccgcagacg gacgaggtcg tccgtccact cctgcggttc ctgcggctcg
     7801 gtacggaagt tgaccgtgct tgtctcgatg tagtggttga cgatggtgca gaccgccggc
     7861 atgtccgcct cggtggcacg gcggatgtcg gccgggcgtc gttctgggct catggtagat
     7921 cccccgttcg taaatggtga aaattttcag aaaattgctt ttgctttaaa agaaatgatt
     7981 taaattgctg caatagaagt agaatgcttg attgcttgag attcgtttgt tttgtatatg
     8041 ttgtgttgag aattaattct cgagcctaga gtcgagatct ggattgagag tgaatatgag
     8101 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
     8161 tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
     8221 tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg
     8281 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
     8341 gactccctta attctccgct catgatcttg atcccctgcg ccatcagatc cttggcggca
     8401 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     8461 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
     8521 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     8581 agctgacatt catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct
     8641 tcctttagca gcccttgcgc cctgagtgct tgcggcagcg tgaagcttgc atgcctgcag
     8701 gtcgactcta gaggatcccc gggtaccgag ctcgaattcg taatcatgtc atagctgttt
     8761 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8821 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8881 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8941 gggagaggcg gtttgcgtat tggagcttga gcttggatca gattgtcgtt tcccgccttc
     9001 agtttaaact atcagtgttt gacaggatat attggcgggt aa
//
