LOCUS       sequence-263851-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a 
            N-terminal hexa-Histidine tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Ranaghan MJ, Durney MA, Mesleh MF, McCarren PR, Garvie CW, Daniels 
            DS, Carey KL, Skepner AP, Levine B, Perez JR
  TITLE     The Autophagy-Related Beclin-1 Protein Requires the Coiled-Coil and 
            BARA Domains To Form a Homodimer with Submicromolar Affinity.
  JOURNAL   Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 
            10.1021/acs.biochem.7b00936. Epub 2017 Dec 14.
  PUBMED    29185708
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.7b00936"; journalName: "Biochemistry"; date: 
            "2017-12-26- 26"; volume: "56"; issue: "51"; pages: "6639-6651"
COMMENT     Sequence Label: BPV00554 pET21GG2-His(6)-BECN(248-450)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             95..112
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             119..136
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             143..163
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      525..534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             797..820
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             821..865
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gagccatcat catcaccatc atggatccct
      121 ggttccgcgt ggtagcgcta gcgaaaatct gtattttcag ggaaaaagcg ttgaaaatca
      181 gatgcgttat gcacagaccc agctggacaa actgaaaaaa acgaatgttt ttaacgccac
      241 ctttcacatt tggcatagtg gtcagtttgg caccattaat aactttcgtc tgggtcgtct
      301 gccgagcgtt ccggttgaat ggaatgaaat taatgcagca tggggtcaga cagttctgct
      361 gctgcatgca ctggccaata aaatgggtct gaaattccag cgttatcgtc tggttccgta
      421 tggtaatcat agctatctgg aaagcctgac cgataaaagc aaagaactgc cgctgtattg
      481 tagcggtggt ctgcgttttt tttgggataa caaatttgat catgccatgg tggcatttct
      541 ggattgtgtt cagcagttta aagaagaggt tgaaaaaggc gaaacccgtt tttgtctgcc
      601 gtatcgtatg gatgtagaaa aaggcaaaat tgaagatacc ggtggtagtg gtggtagcta
      661 tagcattaaa acccagttta atagcgaaga acagtggacc aaagccctga aatttatgct
      721 gaccaatctg aaatggggtc tggcatgggt tagcagccag ttttataaca aataaagcct
      781 cgagggtggt ggtagcgatt ataaagatga tgatgataaa ggcctgaacg atatctttga
      841 agcccagaaa attgaatggc acgagtaa
//
