LOCUS       sequence-263858-        3978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC36A1
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2103..2202)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2219..2238)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2220..2238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2243..2259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2243..2259)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2361..2380)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2372..3181
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2439..2458)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3328..3916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3817..3836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgtcca cccagcggct gagaaacgag gactaccacg attatagctc
      721 cacagacgtg tctcctgagg agagcccatc cgagggcctg aacaatctgt ctagccctgg
      781 cagctaccag aggttcggcc agtctaatag caccacatgg tttcagaccc tgatccacct
      841 gctgaagggc aacatcggaa caggactgct gggactgcca ctggcagtga agaatgccgg
      901 catcgtgatg ggccccatca gcctgctgat catcggcatc gtggccgtgc actgcatggg
      961 catcctggtg aagtgcgccc accacttctg tcggagactg aacaagtcct ttgtggacta
     1021 cggcgataca gtgatgtatg gcctggagtc ctctccatgt tcttggctca gaaatcacgc
     1081 ccattggggt aggcgcgtgg tggacttctt tctgatcgtg acccagctgg gcttctgctg
     1141 cgtgtacttc gtgtttctgg ccgataactt taagcaggtc atcgaggccg ccaatggcac
     1201 cacaaacaat tgccacaaca atgagacagt gatcctgacc cctacaatgg attccagact
     1261 gtatatgctg tctttcctgc catttctggt gctgctggtg ttcatcagga acctgcgcgc
     1321 cctgtccatc ttttctctgc tggccaatat caccatgctg gtgtccctgg tcatgatcta
     1381 ccagttcatc gtgcagagga tcccagaccc atctcacctg ccactggtgg caccttggaa
     1441 gacctatccc ctgttctttg gcacagccat cttcagcttt gagggcatcg gcatggtgct
     1501 gcctctggag aacaagatga aggatccacg caagttcccc ctgatcctgt acctgggcat
     1561 ggtcatcgtg accatcctgt acatcagcct gggctgcctg ggctatctgc agtttggcgc
     1621 caatatccag ggctccatca cactgaacct gcccaattgt tggctgtacc agagcgtgaa
     1681 gctgctgtat tccatcggca tcttctttac ctacgccctg cagttctatg tgcccgccga
     1741 gatcatcatc cccttcttcg tgagcagggc accagagcac tgcgagctgg tggtggacct
     1801 gttcgtgaga accgtgctgg tgtgcctgac atgtatcctg gccatcctga tcccccggct
     1861 ggacctggtc atcagcctgg tgggatccgt gagctcctct gccctggccc tgatcatccc
     1921 ccctctgctg gaggtgacca cattctattc tgagggcatg agccctctga ccatctttaa
     1981 ggacgccctg atcagcatcc tgggcttcgt gggctttgtg gtgggcacat acgaggccct
     2041 gtatgagctg atccagcctt ctaacgcccc aatcttcatc aatagcacct gtgcctttat
     2101 cgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2161 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2221 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2281 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2341 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2401 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2461 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2521 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2581 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2641 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2701 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2761 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2821 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2881 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2941 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3001 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3061 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3121 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3181 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3241 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3961 gccttttgct cacatgtt
//
