LOCUS       sequence-263911-        5094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLCO5A1
FEATURES             Location/Qualifiers
     source          1..5094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3219..3318)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3335..3354)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3336..3354)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3359..3375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3359..3375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3477..3496)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3488..4297
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3555..3574)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4444..5032
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4933..4952
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggatg agggcaccgg cctgcagcca ggagcaggag agcagctgga
      721 ggcaccagca accgccgagg cagtgcagga gcggtgcgag cctgagacac tgagatctaa
      781 gagcctgcca gtgctgagct ccgccagctg caggccatcc ctgtctccta cctccggcga
      841 tgccaatcct gccttcggct gcgtggactc tagcggccac caggagctga agcagggacc
      901 taacccactg gcaccaagcc cttccgcccc atctaccagc gccggactgg gcgattgtaa
      961 tcacagggtg gacctgtcta agacattcag cgtgtcctct gccctggcca tgctgcagga
     1021 gcggagatgc ctgtacgtgg tgctgaccga ttcccgctgt tttctggtgt gcatgtgctt
     1081 cctgaccttc atccaggccc tgatggtgag cggctacctg agctccgtga tcaccacaat
     1141 cgagaggcgc tattccctga agtctagcga gagcggcctg ctggtgtcct gcttcgacat
     1201 cggcaacctg gtggtggtgg tgtttgtgag ctacttcgga ggaagaggcc ggagaccact
     1261 gtggctggca gtgggaggac tgctgatcgc ctttggcgcc gccctgttcg cactgccaca
     1321 ctttatctcc cccccttatc agatccagga gctgaacgcc tctgccccaa atgatggact
     1381 gtgccaggga ggaaattcta ccgccacact ggagccacca gcatgtccaa aggacagcgg
     1441 aggaaacaat cactgggtgt atgtggccct gttcatctgc gcccagatcc tgatcggcat
     1501 gggctccacc cctatctaca cactgggccc aacctatctg gacgataacg tgaagaagga
     1561 gaattcctct ctgtacctgg ccatcatgta tgtgatgggc gccctgggac cagcagtggg
     1621 atacctgctg ggcggcctgc tgatcggctt ttatgtggat cctaggaacc cagtgcacct
     1681 ggatcagaat gaccctcgct tcatcggcaa ctggtggagc ggctttctgc tgtgcgccat
     1741 cgccatgttt ctggtcatct tcccaatgtt cacctttccc aagaagctgc ctccaaggca
     1801 caagaagaag aagaagaaga agttcagcgt ggatgccgtg agcgacgatg acgtgctgaa
     1861 ggagaagagc aacaattccg agcaggccga caagaaggtg agctccatgg gctttggcaa
     1921 ggatgtgcgc gacctgccta gggcagccgt gagaatcctg tctaacatga ccttcctgtt
     1981 cgtgagcctg tcttacacag ccgagagcgc catcgtgaca gcctttatca ccttcatccc
     2041 aaagttcatc gagtcccagt ttggcatccc cgcctctaat gccagcatct acaccggcgt
     2101 gatcatcgtg ccaagcgccg gagtgggaat cgtgctgggc ggctatatca tcaagaagct
     2161 gaagctggga gcaagggagt ccgccaagct ggccatgatc tgctccggcg tgtctctgct
     2221 gtgcttttct accctgttca tcgtgggctg cgagagcatc aacctgggcg gcatcaatat
     2281 cccttacacc acaggcccaa gcctgacaat gccccaccgc aacctgaccg gctcctgcaa
     2341 cgtgaattgc ggctgtaaga tccacgagta cgagcccgtg tgcggatccg acggaatcac
     2401 ctatttcaat ccatgcctgg caggatgcgt gaactccgga aatctgtcta caggcatccg
     2461 gaactatacc gagtgcacat gcgtgcagtc cagacaggtc atcacccccc ctacagtggg
     2521 ccagaggtct cagctgcgcg tggtcatcgt gaagacctac ctgaacgaga atggctatgc
     2581 cgtgagcggc aagtgcaagc ggacctgtaa cacactgatc cccttcctgg tgttcctgtt
     2641 tatcgtgacc tttatcacag catgcgcaca gccaagcgcc atcatcgtga cactgaggtc
     2701 cgtggaggat gaggagcgcc ctttcgccct gggcatgcag tttgtgctgc tgcggacact
     2761 ggcctacatc ccaaccccca tctatttcgg cgccgtgatc gacaccacat gtatgctgtg
     2821 gcagcaggag tgcggagtgc agggatcttg ttgggagtac aacgtgacca gcttcagatt
     2881 cgtgtacttc ggactggcag caggcctgaa gtttgtgggc ttcatcttta tcttcctggc
     2941 ctggtacagc atcaagtata aggaggatgg actgcagagg cgccggcagc gggagttccc
     3001 cctgagcacc gtgtccgaga gagtgggaca cccagacaat gccagaacaa ggtcctgccc
     3061 tgccttctct acccagggcg agtttcacga ggagacaggc ctgcagaagg gcatccagtg
     3121 tgccgcccag acataccctg gcccatttcc agaggcaatc tctagctccg ccgacccagg
     3181 cctggaggag tcccctgccg ccctggagcc accctctgac ccagctttct tgtacaaagt
     3241 tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     3301 aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt
     3361 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     3421 agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     3481 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     3541 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     3601 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     3661 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     3721 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     3781 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     3841 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
     3901 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
     3961 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
     4021 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
     4081 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
     4141 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
     4201 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
     4261 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
     4321 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
     4381 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4441 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4501 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4561 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4621 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4681 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4741 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4801 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4861 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4921 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4981 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5041 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
