LOCUS       sequence-263924-        9741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Sulahian R, Kwon JJ, Walsh KH, Pailler E, Bosse TL, Thaker M, 
            Almanza D, Dempster JM, Pan J, Piccioni F, Dumont N, Gonzalez A, 
            Rennhack J, Nabet B, Bachman JA, Goodale A, Lee Y, Bagul M, Liao R, 
            Navarro A, Yuan TL, Ng RWS, Raghavan S, Gray NS, Tsherniak A, 
            Vazquez F, Root DE, Firestone AJ, Settleman J, Hahn WC, Aguirre AJ
  TITLE     Synthetic Lethal Interaction of SHOC2 Depletion with MEK Inhibition 
            in RAS-Driven Cancers.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):118-134.e8. doi: 
            10.1016/j.celrep.2019.08.090.
  PUBMED    31577942
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "118-134.e8"
COMMENT     Sequence Label: pLEX305_FKBP12F36V-SHOC2
FEATURES             Location/Qualifiers
     source          1..9741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3..20
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    137..158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(173..178,179..196,197..203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    211..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     216..238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     235..251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     235..251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     270..290
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        272..290
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        318..544
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             545..725
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    772..897
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1390..1623
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1808..1852
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2083..2293
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2105..2301
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2152..2287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2333..2935
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2333..2352)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2829..2849
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2978..3095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3153..3653
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3572..3590
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(3662..3664,3665..3985)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             3992..4018
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             3992..4018
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4022..4048
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4064..4088
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5835..5859)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5911..6499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5964..5984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6382..6393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6571..6804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6882..7003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6919..6938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6973..6992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7043..7178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7198..7217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7199..7217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7227..7244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7227..7243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7236..7258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7385..7840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7472..7491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7682..7703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7866..7970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7971..8039,8040..8831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8189..8208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9002..9590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9491..9510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
       61 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
      121 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
      181 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
      241 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
      301 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
      361 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      421 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      481 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      541 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      721 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      781 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      841 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      901 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      961 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1021 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1081 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1141 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1201 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     1261 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1321 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1381 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1441 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1501 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1561 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1621 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1681 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1741 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1801 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1861 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1921 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1981 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
     2041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2101 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     2161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     2221 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     2281 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
     2341 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     2401 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     2461 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     2521 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
     2581 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     2641 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     2701 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     2761 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     2821 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     2881 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
     2941 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3001 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3061 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3121 atccactttg gcgccggctc gaggcctgca ggttgcgcct tttccaaggc agccctgggt
     3181 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     3241 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     3301 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     3361 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     3421 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     3481 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     3541 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     3601 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc caggggctag
     3661 catgggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg
     3721 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaag ttgattcctc
     3781 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
     3841 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
     3901 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
     3961 cgatgtggag cttctaaaac tggaaggcgg ctacccctac gacgtgcccg actacgccgg
     4021 ctatccgtat gatgtcccgg actatgcagg catcgataga tcaacaagtt tgtacaaaaa
     4081 agttggcatg agtagtagtt taggaaaaga aaaagactct aaagaaaaag atcccaaagt
     4141 accatcagcc aaggaaagag aaaaggaggc aaaagcctct ggaggttttg ggaaagagag
     4201 caaagaaaaa gaacctaaga ccaaagggaa agatgccaaa gatggaaaga aggactccag
     4261 tgctgcccaa ccaggggtgg cattttcagt tgacaatacg atcaaacggc caaacccagc
     4321 acctgggact agaaaaaaat ccagcaatgc agaggtgatt aaagagctca acaaatgccg
     4381 ggaagagaat tcaatgcgtt tggacttatc caagagatct atacacatat tgccatcatc
     4441 aatcaaagag ttgactcaat taacagaact ttatttatac agtaacaaat tgcagtccct
     4501 cccagcagag gtgggatgtt tagtaaatct catgacactg gctctaagtg aaaattcact
     4561 taccagtttg cctgactctc ttgataactt gaagaagctg cggatgcttg atttacggca
     4621 taataaactg agagaaattc cttcagtggt gtataggctg gattctctca ccactcttta
     4681 tcttcgattt aatcgtataa ctactgtgga aaaggacatc aaaaacttgt caaaactcag
     4741 catgcttagc attcgagaga acaaaattaa acaactacct gctgaaattg gtgaattatg
     4801 taatctcatc acgctggatg tagctcacaa tcaacttgaa caccttccaa aggagattgg
     4861 aaactgtaca cagataacca accttgactt gcagcacaat gaactgctag acctcccaga
     4921 tactatagga aacctgtcca gtttaagtcg tcttggtctg agatataaca gactgtcagc
     4981 aatacccaga tcattagcaa aatgcagtgc acttgaagaa ttaaatttag agaacaataa
     5041 catttctact ttaccagaga gtcttttatc aagtcttgtg aaactgaata gtttgacctt
     5101 agctagaaat tgcttccagt tgtatccagt gggtggtcca tctcagtttt ctaccatcta
     5161 ttccctcaac atggaacaca atcgaatcaa caaaattcca tttggaattt tctccagagc
     5221 aaaagtatta agtaagctga atatgaagga caatcagtta acatcacttc ccttggattt
     5281 tggaacttgg accagtatgg tagaattgaa tttagccact aatcagctca caaagatccc
     5341 tgaggatgtg tctggtctcg tttctcttga ggttcttatc ttatcaaaca atcttctaaa
     5401 gaagcttccc catggtcttg gaaaccttag gaagttaaga gagttggatc tagaagagaa
     5461 caaattggaa tccttgccaa atgaaattgc atatcttaag gatttacaga aattagtctt
     5521 gacaaacaac cagttgacca ctcttcccag aggcattggt caccttacta atctcacaca
     5581 tctgggcctt ggagagaacc tacttactca ccttcctgaa gaaattggta cactggagaa
     5641 cctagaagaa ctgtatttga atgacaaccc caacctgcat agccttccct ttgagctggc
     5701 actctgcagc aagctttcaa tcatgagtat tgagaactgt ccactcagtc accttccacc
     5761 tcagattgtt gctggggggc cttctttcat cattcagttc ttaaagatgc agggtccata
     5821 tcgtgccatg gtctgcccaa ctttcttgta caaagtggtt accggttagt aatgaactag
     5881 ttaagtcgac aatcaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     5941 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     6001 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     6061 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     6121 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     6181 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6241 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6301 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6361 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6421 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6481 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     6541 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6601 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6781 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     6841 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6901 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6961 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     7021 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     7081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7141 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7201 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7381 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7441 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7681 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7741 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7801 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9721 gccgcagccg aacgaccgag c
//
