LOCUS       sequence-263971-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr1256.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr1256
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(644..662)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(645..662)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(693..715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    704..720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(728..734,735..752,753..758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    773..794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(911..928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gggcgattgg gcccgacgtc gcatgctccc ggccgccatg gcggccgcgg gaattcgatt
       61 ggtgatggcc tatgatcgtt atgtggccat ctgtaagccc ttgcactatt caactatcat
      121 gaagcaatgg gtttgtgttg tgctgttgat attatcttgg atcggaggat ttttgcactc
      181 agtaattcag ctcagtacta tttatggact tccattctgt ggtcctaata tcattgatca
      241 ttttatgtgt gacatgtacc ccttactgaa acttgtttgt attgacacct atgtcattgg
      301 cctcttagtg atggccaatg gagggctgat ctgcacggtt gtgtttatac tcctgctcat
      361 ctcttatggt gtcatcctgt actctctaaa gaaccttaat caggaaggga ggtggaaagc
      421 tctctctacc tgtggctccc acatcactgt agttgtttca ttctttgttc cctgtatttt
      481 catgtatgca cgacctgcta agactttccc cattgacaaa atgttgagtg tgttttatac
      541 agtcattacc cccatgatga accaatcact agtgaattcg cggccgcctg caggtcgacc
      601 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      661 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1081 ttttccatag gctccgcccc ctgacgagca tcmcaaaaat cgacgctcaa gtcaaaggtg
     1141 gcgaaacccg acaggactat aagataccag gcgtttcccc tggaagctcc ctcgngs
//
