LOCUS       sequence-263978-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr653.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr653
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(821..839)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(822..839)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(857..873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(857..873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(870..892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    881..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(905..911,912..929,930..935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    950..971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1088..1105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgattgggc ccgacgtcgc atgctcccgg ccgccatggc ggccgcggga attcgattaa
       61 agcggtgagt gaaaaatgaa aagaatgcgg ggatatagaa gacaagaatt acacaaatgt
      121 gggctccaca tgtgctgaat gctcggaact gggcatcctt tgagggcagt cgcagcacgg
      181 cctgcagaat caggatgtag gaggttgcaa tgaggaccac atccatgcca gtgactgaaa
      241 gtgctacagt aagaccatag atggtgttag gcttgatgct ggcacaggcg agcttggcta
      301 tgcccatgtg ctcacagtaa gtgtgaggga ttatatgatg tccacagaag ggtaaacgtt
      361 caatgagaat gacaaagggg aagacaaaga gaaggccacg agtcacgcat gccaggccaa
      421 tctttccaat tacaacagca ttgaggatgg atgtatagtg gagtggtctg cagatagcaa
      481 catatcgatc aaaagccatg gccagcagaa cagctgactc tgtggcaaag actgcatgaa
      541 caaaaaacat ctgagtgagg cagccatggt aggaaatgac atgggatctg agccagaata
      601 ttgccagcat tttaggcaat gttgttgaac agagaaccag atcagtgata gaaagcagac
      661 acaagaagag gtacattggc tcatggagag ccctgtctgt tataatgata tagaggatgg
      721 taccatttcc aaccagagca aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      781 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1141 ctcactcaaa ggcggtaata cggttatccm nnaatcagg
//
