LOCUS       sequence-264029-        2013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein FGFR N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTetD-SNAP-FOP
FEATURES             Location/Qualifiers
     source          1..2013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      872..881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc accatggcgg cgacggcggc cgcagtggtg
      901 gccgaggagg acacggagct gcgggacctg ctggtgcaga cgctggagaa cagcggggtc
      961 ctgaaccgca tcaaggctga actccgagca gctgtgtttt tagcactaga ggagcaagaa
     1021 aaagtagaga acaaaactcc tttagttaat gagagcctga gaaagttttt aaataccaaa
     1081 gacggtcgtt tagtggctag tcttgttgca gaatttcttc agttttttaa ccttgacttt
     1141 actttggctg tttttcaacc tgaaactagc acactgcaag gtctcgaagg tcgagagaat
     1201 ttagcccgag atttaggtat aattgaagca gaaggtactg tgggtggacc cttattatta
     1261 gaagtgatca ggcgctgtca acagaaagaa aaagggccaa ccactgggga aggtgcactt
     1321 gatctatctg atgtacattc tccaccaaag tcaccagagg gaaaaacaag tgcacagaca
     1381 acaccaagta agaaggccaa tgatgaggcc aatcagagtg atacaagtgt ctccttgtca
     1441 gaacccaaga gcaaaagcag ccttcactta ctgtcccatg aaacaaaaat tggatctttt
     1501 ctaagcaaca gaactttaga tggcaaagac aaagctggcc tttgtccaga tgaagatgat
     1561 atggaaggag attctttctt tgatgatccc attcctaagc cagagaaaac ttacggtttg
     1621 aggaatgaac ctaggaagca agcaggaagt ctggcctcgc tctcggatgc acccccctta
     1681 aaaagtggac tcagctccct ggcgggagcc ccttctttaa aagactctga gagtaaaagg
     1741 ggaaatacag ttttgaaaga tctgaaattg atcagtgata aaattggatc acttggatta
     1801 ggaactggag aagatgatga ctatgttgat gattttaata gtaccagcca tcgctcagag
     1861 aaaagtgaga taagtattgg tgaagagata gaagaagacc tttctgtgga aatagatgac
     1921 atcaatacca gtgataagct tgatgacctc acacaagatc tgactgtatc ccagctcagt
     1981 gatgttgcgg attatctgga agatgttgca tag
//
