LOCUS       sequence-264079-        2181 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the centrosomal protein Cep128 N-terminally 
            fused to SNAP-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Lukinavicius G, Lavogina D, Orpinell M, Umezawa K, Reymond L, Garin 
            N, Gonczy P, Johnsson K
  TITLE     Selective chemical crosslinking reveals a Cep57-Cep63-Cep152 
            centrosomal complex.
  JOURNAL   Curr Biol. 2013 Feb 4;23(3):265-70. doi: 10.1016/j.cub.2012.12.030. 
            Epub 2013 Jan 17.
  PUBMED    23333316
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2012.12.030"; journalName: "Curr Biol"; date: 
            "2013-02-4- 4"; volume: "23"; issue: "3"; pages: "265-70"
COMMENT     Sequence Label: pEBTet-SNAP-Cep128
FEATURES             Location/Qualifiers
     source          1..2181
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..45
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    47..65
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    68..86
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     96..120
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             220..243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             244..261
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             811..834
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     protein_bind    847..871
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc tatcagtgat
       61 agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa ccgtcagatc
      121 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
      181 ctccggactc tagcgtttaa acttaagctt accaccatgg attataaaga cgatgatgac
      241 aagcaccacc accaccacca cgacaaggat tgtgaaatga aacgcaccac actggacagc
      301 cctttgggga agctggagct gtctggttgt gagcagggtc tgcacgaaat aatttttctg
      361 ggcaagggga cgtctgcagc tgatgccgtg gaggtcccag cccccgctgc ggttctcgga
      421 ggtccggagc ccctgatgca ggctacagcc tggctgaatg cctatttcca ccagcccgag
      481 gctatcgaag agttccccgt gccggctctt caccatcccg ttttccagca agagtcgttc
      541 accagacagg tgttatggaa gctgctgaag gttgtgaaat tcggagaagt gatttcttac
      601 tctcacttag cagccctggc aggcaacccc gcagccacgg cagcagtgaa gacggcactg
      661 agtggcaatc ctgtccctat cctgatcccg tgccacagag tggtccaggg ggatttggat
      721 gtgggcggtt acgagggtgg actggccgtg aaggaatggc ttctggccca tgaaggccac
      781 cggttgggga agccaggctt gggattcgaa ctggaggtgc tgttccaggg ccccggtacc
      841 gccatcacaa gtttgtacaa aaaagcaggc ttaatggcag aatcatccag cgaatcagat
      901 cacttccgct gtcgtgaccg attgagtcca tgggctgcca gatcaacgca caggggaact
      961 cgaagtcttc ctacagtaga agttaccgag aaggtcaaca ctataacaag tactttacag
     1021 gataccagtc ggaacctgcg acaagtggac cagatgcttg gacgataccg agaatacagt
     1081 aatggacagg cgggtgcgat agaacattta aaagaaagct tggaacaatc aatcgaccaa
     1141 ctccggagtc aacgtttatt gagaaactca ggagggagaa gtatttctgt tacaagtttg
     1201 agtgcaagtg accttgatgg tggcactggg tcagagctcc atcattttcc acctacctca
     1261 cctctcaagg actatgggga tccacagggt attaaacgaa tgagatcaag aactggtgtc
     1321 cggtttgttc aagagactga tgatatgact cagcttcatg gttttcatca gtctcttcga
     1381 gacctcagca gtgaacaaat tcgccttgga gatgatttca acagggagct ttccagaagg
     1441 agccggtcag atgccgaaac aaaaagggct ttggaagaat tgactgaaaa acttaatgaa
     1501 gcccagaaac aagaagtggt ttcagatcgg gtggagcggc ggcttcagga actggagaga
     1561 gagatgcgca cagaaaggga gctggtggaa agacgccagg atcaactggg actcatgtcc
     1621 ctgcagctac aggaggcact aaagaaacaa gaagctaaag cagatgagca tgagggggca
     1681 ataaaaaata agctaagaca aactgaaact gagaagaatc aacttgaaca ggaattggag
     1741 ctatctcgaa ggttattgaa tcaatcagaa ggcagccgag aaacactttt gcatcaggta
     1801 gaagaactgc gtacacaact tacgaaagca gaaggtgatc gaaagggttt acagcatcaa
     1861 gtatctcaga tttccaagca acagtcaaac tatcaggatg aacaagggga ggactggaga
     1921 tttaggagag gggttgagcg ggaaaaacag gacctggaga agcaaatgtc agatttgaga
     1981 gtgcagctga acttcagcgc aatggcatct gagttagagg aagtgaaacg gtgcatggag
     2041 agaaaagaca aggagagagc acatttggca tcacaagtag agcacatcaa tagaaaatgt
     2101 cttttaaatt tggttcaaga tttggattgt aaagataatg aaatattgac atactcttta
     2161 caaagccctc tacatgttta a
//
