LOCUS       sequence-264237-        2573 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knockin donor vector of miniAID-mClover3 to C-terminus of endogenous
            human CTCF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Hyle J, Zhang Y, Wright S, Xu B, Shao Y, Easton J, Tian L, Feng R, 
            Xu P, Li C
  TITLE     Acute depletion of CTCF directly affects MYC regulation through loss
            of enhancer-promoter looping.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):6699-6713. doi: 
            10.1093/nar/gkz462.
  PUBMED    31127282
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "6699-6713"
COMMENT     Sequence Label: pKnockinDonor-humanCTCF-miniAID-mClover3
FEATURES             Location/Qualifiers
     source          1..2573
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             824..1027
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             join(1034..1036,1037..1039,1040..1753)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1079..1100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1687..1708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggtgatgg cggagccttg tgcagtagaa gggtacttgt gttctgcaag atgggtactg
       61 ttaatgtggg cgggttttga aaaacaacca aagtcacagt cagctggcag aatggatgcg
      121 gtaatgaata taaaagagcc aaatcgtaaa ggttggagag ggaaagcagt ttctgcactg
      181 agattgcccc ttctaagaaa ctcagggtca ggcggtggct catgcctgtg atcccagcac
      241 tttggggggc caaggcggga ggatcacctg aggtcagtag ttccatacca gcctggtcaa
      301 catggtgaaa ccccatctct gctaaaaata caaaaattag ccaggcgtag gggtgggcac
      361 ctgtagtccc atctactcag gaggctgagg caggagaatt gctttaaccc ggggggcaga
      421 ggttgcagtg agctgagatc atgcccgtgc actccagcct ggtcatagag caagactccg
      481 tctcaaaaaa taaaataaaa aataaattga ctgtctctgg accgctatct aataaggctg
      541 tcctgcattg ctgacatccc gttcgctgtc agtctaaaag acccttgtga ttcttggggc
      601 tttaatggac catttgttct gtctgtgctc ttctttgcca gcaacagcta tcattcaggt
      661 tgaagaccag aatacaggtg caattgagaa cattatagtt gaagtaaaaa aagagccaga
      721 tgctgagccc gcagagggag aggaagagga ggcccagcca gctgccacag atgcccccaa
      781 cggagacctc acgcccgaga tgatcctcag catgatggac cggaaggaga agagtgcttg
      841 tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc caccggtgag
      901 atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc cggaggcggc
      961 ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg atttgaggat
     1021 gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1081 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg agggcgaggg
     1141 cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1201 gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca gccgctaccc
     1261 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1321 gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg tgaagttcga
     1381 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1441 catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata tcacggccga
     1501 caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg aggacggcag
     1561 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1621 gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca acgagaagcg
     1681 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg gcatggacga
     1741 gctgtacaag tgatggcgga gccttgtgcg tcgccaggac ttctctgggc tgtgtttaaa
     1801 cggcccgcat cttaattttt ctcccttctt tctttttttg gctttgggaa aagcatcatt
     1861 ttaccaaaca taccgagaac gaaaacttca aggatgatgt tagaaaaaaa tgtgatttaa
     1921 ctagaacttg ctgtctgatg ttagcaaatc atggaatgtt ctgagtccct gagggtttac
     1981 tgtgaagtgc tgaggacagt gttgacaact aactcgtttt cctagatgga aacggagaca
     2041 ttgacccctc cctccatgtg gtaaaccact ccagaatggc caccaggctt cccagagttc
     2101 tatggtcttc ttcccaagag agtttttaat tgtaaatgca tacttgggaa ggacttagag
     2161 ttttaaactg ttttttgctt ttgcttttcc ctgactccct ttgcttggag tcagctgcac
     2221 accagtagta tggcatgcta cgatcaggtt ctgtcctgaa agctttgcct ctttcttggc
     2281 aaagtttctg gtatggtcaa gcttgtaaat aacttttttt acattttaat cttttccatt
     2341 aattaagagg ttgaaaagaa gtgcagtgta agaaaaccca gcattttaat tacttgcaaa
     2401 ttaagttacc acagactctg tagtgtgtaa atgttgacaa ggaattggat cacaatcatg
     2461 tagcagaatg gcacccagac cactgcccac cagtgacgga catgcacgtg gcagatcatg
     2521 atttccagcc cacggagcca gcatttgaac gcacaaggct ccgccatcac cgg
//
