LOCUS       sequence-264289-        1992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ER-targeted Ribosome non-stalling reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wang L, Xu Y, Rogers H, Saidi L, Noguchi CT, Li H, Yewdell JW, 
            Guydosh NR, Ye Y
  TITLE     UFMylation of RPL26 links translocation-associated quality control 
            to endoplasmic reticulum protein homeostasis.
  JOURNAL   Cell Res. 2020 Jan;30(1):5-20. doi: 10.1038/s41422-019-0236-6. Epub 
            2019 Oct 8.
  PUBMED    31595041
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-019-0236-6"; journalName: "Cell Res"; date: 
            "2020-01"; volume: "30"; issue: "1"; pages: "5-20"
COMMENT     Sequence Label: ER_K0
FEATURES             Location/Qualifiers
     source          1..1992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(118..120,121..831)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLKE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(160..181)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(421..440)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             838..903
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1285..1992
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1427..1445)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1695..1714
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtaccgt gcacgctgct cctgctgttg gcggccgccc tggctccgac tcagacccgc
       61 gccggctctc actcccaaga tcttccagga aacgataaca gtacagcagg aggtagtgtg
      121 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      181 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      241 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      301 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      361 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      421 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      481 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      541 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      601 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      661 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      721 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtccttaag
      781 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggagac
      841 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac
      901 aaggccggat ctcctggtcc gacccccagt ggcactaacg tgggatcctc actctctgac
      961 gaggacttca aggctgtttt tggcatgacc cgctctgcct ttgccaactt gcccttgtgg
     1021 aaacagcaga acctcaagaa ggagaaagga ctcttcggat cagggcgctc tcccagcaaa
     1081 gcagtggcca cccgggcggc gggatccact gtccggcaga ggaaaaatgc cagctgtggg
     1141 acaaggagtg caggccgcac aacctcggca ggcaccgggg ggatgtggcg attctacaca
     1201 gaagattcac ctgggctcaa agttggccct aggtcgaccg ccatggcgac ccccggggga
     1261 tccggtaccg gaagcggact cgaggtgagc aagggcgagg aggataacat ggccatcatc
     1321 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     1381 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     1441 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     1501 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     1561 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     1621 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     1681 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     1741 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     1801 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     1861 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     1921 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     1981 ctgtacaagt aa
//
