LOCUS       sequence-264483-       11225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Gregory PA, Bert AG, Paterson EL, Barry SC, Tsykin A, Farshid G, 
            Vadas MA, Khew-Goodall Y, Goodall GJ
  TITLE     The miR-200 family and miR-205 regulate epithelial to mesenchymal 
            transition by targeting ZEB1 and SIP1.
  JOURNAL   Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30.
  PUBMED    18376396
REFERENCE   2  (bases 1 to 11225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2008-05"; volume: "10(5)"; pages: "593-601. Epub 2008
            Mar 30"
COMMENT     Sequence Label: pLenti 4.1 Ex miR200b-200a-429
FEATURES             Location/Qualifiers
     source          1..11225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1447..1680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1865..1909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2207..2324
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    2398..2605
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2594..2611
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(2624..3337,3338..3340,3341..3343))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLGYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2669..2690)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3018..3037
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3277..3298
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3340..3349
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6020..6053
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6076..6279)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        6280..6583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(6633..6666)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6688..7187
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6723..6746)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7120..7139
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7208..7807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7208..7227)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7704..7724
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7877..8465
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7930..7950)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8348..8359)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8468..8484)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8469..8485)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8994..9174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(9236..9824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9316..9335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9995..10786,10787..10855))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10618..10637
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10856..10960)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11035..11054)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
     1321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     1381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     1441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     1501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     1561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     1621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     1681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     1741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     1801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     1861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     1921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     1981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     2041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     2101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     2161 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2221 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2281 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2341 acagcagaga tccagtttgg ttagtaccgg gcccgctcta gaatagagcc caccgcatcc
     2401 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     2461 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     2521 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     2581 gatggctggc aactagaagg cacagtcgag gctggcggcc gctttacttg tacagctcgt
     2641 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     2701 tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca
     2761 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     2821 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     2881 ccatgatata gacgttgtgg ctgttgtagt tgtaccccag cttgtgcccc aggatgttgc
     2941 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     3001 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     3061 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     3121 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     3181 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     3241 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     3301 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg cccgggggat
     3361 ctagctgtgg aaaaaaaagg gacaggataa gtatgatatt gtcaaggaaa ccctgaattg
     3421 ctcgagttaa ttaaggccac ccgggcctgc aggattcgaa tcggcgcgcc taggctccgg
     3481 gtatctgtga ctgtgacccc aggggccagg ctgatagcac ggggtccaca gcatctgccc
     3541 ggggactaca gctcgagggc cccaggaggc cgggtgggca caggccatgg cgagggtggg
     3601 gcacaagagc cccagacccc ggcggctttg cactgatggg ctgcggatgg gcacaggcca
     3661 tagtgagggg ggcatgagag ccccagaccg ggcggctttg cactgatgag ctgcagggca
     3721 ggcagctctc tcctctaacg gtgatcaggc agcggatgga cggttttacc agacagtatt
     3781 agacagaggg ccaggtctaa ccatgtctgg taagacgccc atcggccggc ggtggtgtgg
     3841 gtggcctccg ggtcctgggc tggtgtctgc ggggcctgtg aagggaaggc cccgggggcg
     3901 gctcctgcca cctcctgcag ctgccctgat ccctcctggg tctgcagaac gagcccccga
     3961 ggcacttccc tgacagcccc catcccagca cctggtccca gcccagcctg gctgtgtggg
     4021 ggtacccggg agccacctgg tcacccaggc cctgctcttg gcaaaggttc cctgcagccc
     4081 ctcgctggct tcctgaagct cggtcgggtt aatgagtaac agtgaagggc agcctctccc
     4141 cggtggctgc cccctcccca ggctggggcc ttgtgtgggg gtgcggtccc aggcgccctg
     4201 gttgcagatg gaaaagatga aacaatgggt catcctggcc tggtgggttt tggacaacgt
     4261 tcctgttctt gttgactggg ggaaggaatg agaccccttg tccctcagag ggcaagcgca
     4321 gagcgtcccc gctcccctgc atggtggggc tcgggaatct gaacccacct tgtccttcct
     4381 gcgcccccag cagaccctcc acggagcccg gaccacgtga gagaggccac tgttgctctt
     4441 ccctctccag ccctggcatc ccagccctat ggcctttcct ggagccaggt caccctaaaa
     4501 cccagcctgg gaggttgggt gggggtgagg cacagcggct ccgggagggg gagcaggatg
     4561 ccctccaggc cacctgtgga ggcaccaagg ggtcctcaag gtgtcagacc cagcctgggc
     4621 gtgagtgacc ccgggggcac ccccggcaga ggccggcccc tcccctgggg gctgggcttc
     4681 ggagctgaca caggccctct gccgcccgct cagccctccg ccctcacccg tctgctggcc
     4741 ccgctcrgcc ctccgccctc acccgtctgc tggccccgct cggccctccg ccctcacccg
     4801 tctgctggcc ccgctcggcc ctccgcccca gcccatccct ggagtaggag ctccggatgt
     4861 gcctcggtgg tgtccccggc gagcggcggg tcacctttga acatcgttac cagacagtgt
     4921 tagagtcaag ctgggaaatc cagcactgtc cggtaagatg ctcacagggg cccgggaggg
     4981 acgtggggtc ccgccaggca ggcaggggct gctgtggaag cccagggaag aaccggagcg
     5041 gtgagcagcc aggggtggtg ccgggaccgg acagggctgg cggagagggg cttgggggcc
     5101 ctgagactgc acacaggccc accctccctg cttcctccag ggcaagggga ccctggggct
     5161 gctgacccgg gaccaaggcg ggggcactgg tctgctcggt ggccggccca ccctgtccgg
     5221 caggagggaa gatggctgtg gccaagctgg gtggggcggc ttcctctggg ttccacgtgg
     5281 ctcccggttg ggccttgccc tcctgagccc tctgttgggt cctgctcggc ccgcacctgc
     5341 tgggtgtgac agcagctccc gcccaggcgc tgtgggcagg gcccggacac ctgagcgtcc
     5401 taatccccag atcactgtgg ggcgccctgg gtgcagctgc aggaggcctt gcattccggg
     5461 gtctctgaga tgctggtggg gggcgcccca gcccccttcc gaaggtcacg tccccccgtc
     5521 actctcccca gagccatctg gcccggacgg ggtggggtcc ggtgagcggg ctgtgtggga
     5581 ggggagtgtg gggctcggcg ggaggccctg ggggccggga cggggtcggc cggtcgctgc
     5641 gtgcagggct ccgccgtcat cattaccagg cagtattaga gacctgactc catccaatgc
     5701 tgcccagtaa gatggccacg gctgcccgag ctgggtccgg gggccgcccg gctgagggtt
     5761 gcatgggact cgctgggaag ctcagtagct ggggggcctc gtcctcctgg ggcactgagg
     5821 acagcatcgc cggagggcag gggcccgggt gagccccacc cgccagccct gccgagtcca
     5881 gcagccacca gctttgggtc ctggggtggg cacctggggg gccgaagtgt cctggccaga
     5941 cggccgagga ctaggtcgga gtgggaattc gtcgagattt aaatagatct ccactcacct
     6001 ggtagtagct aggctagcaa taacttcgta taatgtatgc tatacgaagt tatggatctg
     6061 acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc taccgcccat
     6121 ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat tttggtgcca
     6181 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     6241 ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag cgatgactaa
     6301 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     6361 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     6421 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     6481 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     6541 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg
     6601 gctatgaact aatgaccccg taattgatta ctataacttc gtatagcata cattatacga
     6661 agttatctta attaatacaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     6721 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     6781 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     6841 cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt
     6901 gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg
     6961 ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg
     7021 ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca
     7081 ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc
     7141 gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa
     7201 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     7261 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     7321 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     7381 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     7441 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     7501 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     7561 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     7621 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     7681 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     7741 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     7801 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gacaattcga
     7861 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7921 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7981 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8041 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8101 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8161 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8221 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8281 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8341 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8401 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8461 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
     8521 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     8581 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     8641 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     8701 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     8761 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     8821 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     8881 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     8941 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     9001 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9061 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9121 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt
     9181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9421 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9661 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9901 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9961 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10021 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10081 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10141 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10201 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10261 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10321 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10381 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10441 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10501 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10561 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10621 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10681 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10741 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10801 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10861 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10921 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10981 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
    11041 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
    11101 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
    11161 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
    11221 gcgtt
//
