LOCUS       sequence-264566-        3380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human UBA6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Pelzer C, Kassner I, Matentzoglu K, Singh RK, Wollscheid HP, 
            Scheffner M, Schmidtke G, Groettrup M
  TITLE     UBE1L2, a novel E1 enzyme specific for ubiquitin.
  JOURNAL   J Biol Chem. 2007 Aug 10;282(32):23010-4. doi: 
            10.1074/jbc.C700111200. Epub 2007 Jun 19.
  PUBMED    17580310
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.C700111200"; journalName: "J Biol Chem"; date: 
            "2007-08-10- 10"; volume: "282"; issue: "32"; pages: "23010-4"
COMMENT     Sequence Label: pcDNA3.1-HA-UBA6
FEATURES             Location/Qualifiers
     source          1..3380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3348..3365)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttacca tggcctaccc
      121 ctacgacgtg cccgactacg ccgcggtacc tatggaagga tccgagcctg tggccgccca
      181 tcagggggaa gaggcgtcct gttcttcctg ggggactggc agcacaaata aaaatttgcc
      241 cattatgtca acagcatctg tggaaatcga tgatgcattg tatagtcgac agaggtacgt
      301 tcttggagac acagcaatgc agaagatggc caagtcccat gttttcttaa gtgggatggg
      361 tggtcttggt ttggaaattg caaagaatct tgttcttgca gggattaagg cagttacaat
      421 tcatgataca gaaaaatgcc aagcatggga tctaggaacc aacttctttc tcagtgaaga
      481 tgatgttgtt aataagagaa acagggctga agctgtactt aaacatattg cagaactaaa
      541 tccatacgtt catgtcacat catcttctgt tcctttcaat gagaccacag atctctcctt
      601 tttagataaa taccagtgtg tagtattgac tgagatgaaa cttccattgc agaagaagat
      661 caatgacttt tgccgttctc agtgccctcc aattaagttt atcagtgcag atgtacatgg
      721 aatttggtca aggttatttt gtgatttcgg tgatgaattt gaagttttag atacaacagg
      781 agaagaacca aaagaaattt tcatttcaaa cataacgcaa gcaaatcctg gcattgttac
      841 ttgccttgaa aatcatcctc acaaactgga gacaggacaa ttcctaacat ttcgagaaat
      901 taatggaatg acaggtttaa atggatctat acaacaaata acggtgatat cgccattttc
      961 ttttagtatt ggtgacacca cagaactgga accatattta catggaggca tagctgtcca
     1021 agttaagact cctaaaacag ttttttttga atcactggag aggcagttaa aacatccaaa
     1081 gtgccttatt gtggatttta gcaaccctga ggcaccttta gagattcaca cagctatgct
     1141 tgccttggac cagtttcagg agaaatacag tcgcaagcca aatgttggat gccaacaaga
     1201 ttcagaagaa ctgttgaaac tagcaacatc tataagtgaa accttggaag agaagcctga
     1261 tgtaaatgct gacattgtgc attggctctc ttggactgcc caaggctttt tatctccact
     1321 tgctgcagca gtaggaggtg ttgccagcca agaagtattg aaagctgtaa caggaaaatt
     1381 ttctcctttg tgccagtggt tatatcttga agcagcagat attgttgaat cactaggcaa
     1441 acctgaatgt gaagaatttc tcccacgagg agatagatat gatgccttaa gagcttgcat
     1501 tggagacact ttgtgtcaga aactgcaaaa tttaaacatc ttcttagtag ggtgtggagc
     1561 cataggctgt gaaatgttga aaaattttgc tttacttggt gttggcacaa gcaaagagaa
     1621 aggaatgatt acagttacag atcctgactt gatagagaaa tccaacttaa atagacagtt
     1681 cctatttcgt cctcatcaca tacagaaacc taaaagctac actgctgctg atgctactct
     1741 gaaaataaat tctcaaataa agatagatgc acacctgaac aaagtatgtc caaccactga
     1801 gaccatttac aatgatgagt tctatactaa acaagatgta attattacag cattagataa
     1861 tgtggaagcc aggagatacg tagacagtcg ttgcttagca aatctaaggc ctcttttaga
     1921 ttctggaaca atgggcacta agggacacac tgaagttatt gtaccgcatt tgactgagtc
     1981 ttacaatagt catcgggatc ccccagaaga ggaaatacca ttttgtactc taaaatcctt
     2041 tccagctgct attgaacata ccatacagtg ggcaagagat aagtttgaaa gttccttttc
     2101 ccacaaacct tcattgttta acaaattttg gcaaacctat tcatctgcag aagaagtctt
     2161 acagaagata cagagtggac acagtttaga aggctgtttt caagttataa agttacttag
     2221 cagaagacct agaaattggt cccagtgtgt agaattagca agattaaagt ttgaaaaata
     2281 ttttaaccat aaggctcttc agcttcttca ctgtttccct ctggacatac gattaaaaga
     2341 tggcagttta ttttggcagt caccaaagag gccaccctct ccaataaaat ttgatttaaa
     2401 tgagcctttg cacctcagtt tccttcagaa tgctgcaaaa ctatatgcta cagtatattg
     2461 tattccattt gcagaagagg acttatcagc agatgccctc ttgaatattc tttcagaagt
     2521 aaagattcag gaattcaagc cttccaataa ggttgttcaa acagatgaaa ctgcaaggaa
     2581 accagaccat gttcctatta gcagtgaaga tgagaggaat gcaattttcc aactagaaaa
     2641 ggctatttta tctaatgaag ccaccaaaag tgaccttcag atggcagtgc tttcatttga
     2701 aaaagatgat gatcataatg gacacataga tttcatcaca gctgcatcaa atcttcgtgc
     2761 caaaatgtac agcattgaac cagctgaccg tttcaaaaca aagcgcatag ctggtaaaat
     2821 tatacctgct atagcaacaa ccactgctac agtttctggc ttggttgcct tggagatgat
     2881 caaagtaact ggtggctatc catttgaagc ttacaaaaat tgttttctta acttagccat
     2941 tccaattgta gtatttacag agacaactga agtaaggaaa actaaaatca gaaatggaat
     3001 atcatttaca atttgggatc gatggaccgt acatggaaaa gaagatttca ccctcttgga
     3061 tttcataaat gcagtcaaag agaagtatgg aattgagcca acaatggtgg tacagggagt
     3121 caaaatgctt tatgttcctg taatgcctgg tcatgcaaaa agattgaagt taacaatgca
     3181 taaacttgta aaacctacta ctgaaaagaa atatgtggat cttactgtgt catttgctcc
     3241 agacattgat ggagatgaag atttgccggg acctccagta agatactact tcagtcatga
     3301 cactgattaa ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     3361 ttctagttgc cagccatctg
//
