LOCUS       sequence-264805-        5497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  a bidirectional reporter vector allowing the study of biscistronic 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Christophe-Hobertus C, Christophe D
  TITLE     Delimitation and functional characterization of the bidirectional 
            THOX-DUOXA promoter regions in thyrocytes.
  JOURNAL   Mol Cell Endocrinol. 2010 Apr 12;317(1-2):161-7. doi: 
            10.1016/j.mce.2010.01.001. Epub 2010 Jan 12.
  PUBMED    20060878
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2010-04-12"; volume: "317(1-2):161-7. doi"; 
            pages: " 10.1016/j.mce.2010.01.001. Epub 2010 Jan 12"
COMMENT     Sequence Label: pGL4Luc-RLuc
FEATURES             Location/Qualifiers
     source          1..5497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    16..137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(385..402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(556..1144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(636..655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1344..2135,2136..2204))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2309..2357
                     /note="synthetic polyadenylation signal"
     misc_feature    2371..2462
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2411..2430
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2612..2733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2774..3709)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3706..3715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3811..3820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3817..5469
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
       61 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      121 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
      181 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
      241 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
      301 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
      361 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
     1321 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
     1381 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
     1441 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
     1501 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     1561 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     1621 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     1681 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     1741 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     1801 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     1861 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     1921 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     1981 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     2041 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     2101 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     2161 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     2221 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt
     2281 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
     2341 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     2401 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     2461 ctggcctaac tggccggtac cggctctcaa gggcatcggt cgacggatcc ttatcgattt
     2521 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2581 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcaactt ataatggtta
     2641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgctcgaagc ggccggccgc
     2761 cccgactcta gaattactgc tcgttcttca gcacgcgctc cacgaagctc ttgatgtact
     2821 tacccatttc atctggagcg tcctcctggc tgaagtggag gcccttcacc ttcacgaact
     2881 cggtgttagg gaacttctta gctccctcga caatagcgtt ggaaaagaac ccagggtcgg
     2941 actcgatgaa catcttaggc agatcgtcgc tggcccgaag gtaggcgttg tagttgcgga
     3001 caatctggac gacgtcgggc ttgcctccct taacgagagg gatctcgcga ggccaggaga
     3061 gggtaggccg tctaacctcg cccttctcct tgaatggctc caggtaggca gcgaactcct
     3121 caggctccag tttccgcatg atcttgcttg ggagcatggt ctcgacgaag aagttattct
     3181 caagcaccat tttctcgccc tcttcgctct tgatcagggc gatatcctcc tcgatgtcag
     3241 gccactcgtc ccaggactcg atcacgtcca cgacactctc agcatggacg atggccttga
     3301 tcttgtcttg gtgctcgtag gagtagtgaa aggccagaca agccccccag tcgtggccca
     3361 caaagatgat tttctttgga aggttcagca gctcgaacca agcggtgagg tacttgtagt
     3421 gatccaggag gcgatatgag ccattcccgc tcttgccgga cttacccatt ccgatcagat
     3481 cagggatgat gcatctagcc acgggctcga tgtgaggcac gacgtgcctc cacaggtagc
     3541 tggaggcagc gttaccatgc agaaaaatca cggcgttctc ggcgtgcttc tcggaatcat
     3601 agtagttgat gaaggagtcc agcacgttca tttgcttgca gcgagcccac cactgaggcc
     3661 cagtgatcat gcgtttgcgt tgctcggggt cgtacacctt ggaagccatg gtggctttac
     3721 caacagtacc ggattgccaa gctgctagcc tcgaggatat caagatctgg cctcggcggc
     3781 caagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag
     3841 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
     3901 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
     3961 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
     4021 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     4081 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     4141 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     4201 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     4261 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     4321 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     4381 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     4441 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     4501 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     4561 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     4621 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     4681 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     4741 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     4801 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     4861 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     4921 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     4981 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     5041 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     5101 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     5161 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     5221 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     5281 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     5341 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     5401 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     5461 gccgtgtaat aattctagag tcggggcggc cggccgc
//
