LOCUS       sequence-26492-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Boehm JS, Zhao JJ, Yao J, Kim SY, Firestein R, Dunn IF, Sjostrom SK,
            Garraway LA, Weremowicz S, Richardson AL, Greulich H, Stewart CJ, 
            Mulvey LA, Shen RR, Ambrogio L, Hirozane-Kishikawa T, Hill DE, Vidal
            M, Meyerson M, Grenier JK, Hinkle G, Root DE, Roberts TM, Lander ES,
            Polyak K, Hahn WC
  TITLE     Integrative Genomic Approaches Identify IKBKE as a Breast Cancer 
            Oncogene (Kinase Library Clones)
  JOURNAL   Cell. 2007 Jun 15. 129(6):1065-1079.
  PUBMED    17574021
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Jun 15. 129(6):1065-1079."
COMMENT     Sequence Label: pWZL Neo Myr Flag YES1
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             41..82
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             107..130
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctccttctct aggcgccggc cggatccaga attcgccacc atggggtctt caaaatctaa
       61 accaaaggac cccagccagc gccggcgcag gatccgaggt taccttgact acaaagacga
      121 tgacgacaag caattgacaa gtttgtacaa aaaagttggc atgggctgca ttaaaagtaa
      181 agaaaacaaa agtccagcca ttaaatacag acctgaaaat actccagagc ctgtcagtac
      241 aagtgtgagc cattatggag cagaacccac tacagtgtca ccatgtccgt catcttcagc
      301 aaagggaaca gcagttaatt tcagcagtct ttccatgaca ccatttggag gatcctcagg
      361 ggtaacgcct tttggaggtg catcttcctc attttcagtg gtgccaagtt catatcctgc
      421 tggtttaaca ggtggtgtta ctatatttgt ggccttatat gattatgaag ctagaactac
      481 agaagacctt tcatttaaga agggtgaaag atttcaaata attaacaata cggaaggaga
      541 ttggtgggaa gcaagatcaa tcgctacagg aaagaatggt tatatcccga gcaattatgt
      601 agcgcctgca gattccattc aggcagaaga atggtatttt ggcaaaatgg ggagaaaaga
      661 tgctgaaaga ntacttttga atcctggaaa tcaacgaggt attttcttag taagagagag
      721 tgaaacaact aa
//
