LOCUS       sequence-264923-       12410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Numb with mutation at serine 276 converted to aspartic acid cand 
            tagged with GFP cloned into PWPXLD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Abdi K, Neves G, Pyun J, Kiziltug E, Ahrens A, Kuo CT
  TITLE     EGFR Signaling Termination via Numb Trafficking in Ependymal 
            Progenitors Controls Postnatal Neurogenic Niche Differentiation.
  JOURNAL   Cell Rep. 2019 Aug 20;28(8):2012-2022.e4. doi: 
            10.1016/j.celrep.2019.07.056.
  PUBMED    31433979
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-20- 20"; volume: "28"; issue: "8"; pages: "2012-2022.e4"
COMMENT     Sequence Label: PWPXLD-GFP-Numb-S276D
FEATURES             Location/Qualifiers
     source          1..12410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             94..138
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        521..1699
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          752..1690
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1647..1667
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1747..1864
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      1899..1908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1905..1907,1908..1910,1911..2621)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1905..1907,1908..1910,1911..2621)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1950..1971)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2211..2230)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2558..2579
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4672..5260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4725..4745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5143..5154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5349..5582
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5607..5625)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5608..5625)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5915..5934)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6148..6167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6267..6289
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6327..6345)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6413..6517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6518..6586,6587..7378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6736..6755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7549..8137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8038..8057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8291..8308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8378..8398)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8383..8712
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8563..8698
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9846..9911
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10041..10061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10486..10620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10523..10542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10577..10596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10788..11421
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11468..11593
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12086..12319
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
       61 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
      121 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
      181 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
      241 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
      301 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
      361 agatccattc gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgacca
      421 tcgatgtcga cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
      481 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
      541 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      601 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      661 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      721 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      781 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      841 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      901 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      961 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     1021 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1081 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1141 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1201 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1261 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1321 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1381 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1441 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1501 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1561 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1621 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1681 tccatttcag gtgtcgtgag gaatttcgac atttaaattt aattaatctc gacggtatcg
     1741 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1801 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     1861 ttttatcgat cacgagacta gcctcgaggt ttccggtcgc caccatggtg agcaagggcg
     1921 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1981 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     2041 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     2101 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     2161 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     2221 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     2281 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     2341 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     2401 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     2461 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     2521 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     2581 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag
     2641 ctcaagcttc gaattctaac aaactacggc aaagcttcag gagaaagaaa gacgtttatg
     2701 tcccagaggc cagccgtcca catcagtggc agacagatga agaaggagtc cgcactggaa
     2761 agtgtagctt cccagttaag tacctcggcc acgtagaagt tgatgagtca agaggaatgc
     2821 acatctgtga agatgccgta aagagattga aagctgaaag gaagttcttc aaaggcttct
     2881 ttggaaaaac gggaaagaaa gcagtgaagg ccgttctgtg ggtgtcagcg gatgggctca
     2941 gagttgtgga cgagaaaact aaggacctca tagttgacca gacaatagaa aaagtttcct
     3001 tctgtgcccc agataggaac tttgacagag ccttttctta catatgtcgt gatggcacca
     3061 ctcggcgatg gatctgtcat tgtttcatgg ctgtcaaaga cacgggggaa agactgagcc
     3121 acgccgtggg ctgtgctttt gcagcctgtt tagagcgtaa acagaagcgg gagaaggagt
     3181 gtggcgtcac tgctactttt gatgccagta gaaccacttt cacaagagaa ggatcattcc
     3241 gtgtcacaac tgccactgag caagccgaaa gagaggagat catgaaacag ttgcaagatg
     3301 ccaagaaagc tgagacagac aagacagttg ttggtccatc agtggctcct ggcaacactg
     3361 ctccatcccc atcctctccc acctctccca ctccggatgg cactgcatct tcagagatga
     3421 acaatcccca tgctatccca cgccggcatg caccaattga acagcttgct cgtcaaggcg
     3481 atttccgggg atttcctgct cttagccaga agatgtcacc ctttaaacgc cagctgtccc
     3541 tacgcatcaa tgagttgcct tccactatgc agaggaagac cgatttccca ataaaaaaca
     3601 cagtgcccga ggtggaagga gaggccgaga gcatcagctc cttgtgttcc cagatcacca
     3661 gtgccttcag cacgccctct gaggacccct tctcctccgc cccaatgacc aaaccagtga
     3721 cattggtggc accacagtct cctgtgttac aagctaatgg cactgactca gcctcccatg
     3781 tgcttactgc taagccagcc aatactgctc tagcacacgt agcaatgcct gtccgtgaaa
     3841 ccaacccctg ggcccatgtc cctgatgctg ctaacaagga aattgcagcc atacatccgg
     3901 ggactgagtg gggtcagtct tctggtgctg cctctccagg tctcttccag gctggtcaca
     3961 gacgcactcc ctctgaagct gaccgctggt tagaagaagt gtcaaagagt gtgcgggccc
     4021 agcagcctca ggtctcagct gcccctctgc agccagttct ccagccgcct ccgcccgccg
     4081 ccattgcccc tccagcacct cctttccaag gacatgcctt cctcacgtcc cagcctgtgc
     4141 ccgtgggtgt ggtcccaccc ctacaaccag cctttgtccc tacccagtcc taccctgtgg
     4201 ccaacgggat gccctaccca gcctctaatg tgcctgtagt gggcatcacc ccatcccaga
     4261 tggtagccaa tgtgtttggc actgcaggcc accctcagac aactcatcca catcagtcgc
     4321 caagcctggc caagcagcag acattccctc aatatgagac aagtagtgct accaccagtc
     4381 ccttctttaa gcctcctgct cagcacctca atggttctgc agctttcaat ggtgtagaca
     4441 atggtgggct agcctcagga aacaggcatg cagaagtccc tccaggcacc tgcccagtgg
     4501 atcctttcga agctcagtgg gccgcactag aaagcaagtc caagcagcgt acaaatcctt
     4561 ctcctaccaa ccctttctcc agtgacttac agaaaacatt tgaaatagaa ctttaggcaa
     4621 agccgaattc tgcagtcgac ggtaccgcgg gcccgggact agtcatatga taatcaacct
     4681 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4741 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4801 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4861 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4921 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4981 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5041 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5101 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5161 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5221 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcggtacgt atggccaggt
     5281 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5341 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     5401 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5461 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5521 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5581 cagcatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     5641 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     5701 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     5761 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     5821 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     5881 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     5941 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     6001 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     6061 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     6121 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     6181 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     6241 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     6301 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     6361 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6421 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6481 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6541 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6601 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6661 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6721 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6781 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6841 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6901 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6961 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7021 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7081 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7141 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7201 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7261 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7321 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7381 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7441 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7501 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7561 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7621 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7681 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7741 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7801 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7861 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7921 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7981 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8041 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8101 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8161 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8221 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8281 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8341 tctccccgcg cgttggccga ttcattaatg cagctgtgga atgtgtgtca gttagggtgt
     8401 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     8461 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8521 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8581 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8641 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8701 ggcttttgca aaaagcttgg acacaagaca ggcttgcgag atatgtttga gaataccact
     8761 ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg gactcatgtg aaatactggt
     8821 ttttagtgcg ccagatctct ataatctcgc gcaacctatt ttcccctcga acacttttta
     8881 agccgtagat aaacaggctg ggacacttca catgagcgaa aaatacatcg tcacctggga
     8941 catgttgcag atccatgcac gtaaactcgc aagccgactg atgccttctg aacaatggaa
     9001 aggcattatt gccgtaagcc gtggcggtct gtaccgggtg cgttactggc gcgtgaactg
     9061 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     9121 cttaaagtgc tgaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     9181 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc
     9241 accatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     9301 caagatacct ggattgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     9361 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     9421 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     9481 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     9541 taatcacggc gcacaaccgc ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg
     9601 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gcgaaatcct
     9661 cgacgtccag gcacgtattg tgatgagcga tgccgaacgt accgacgatg atttatacga
     9721 tacggtgatt ggctaccgtg gcggcaactg gatttatgag tgggccccgg atctttgtga
     9781 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     9841 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     9901 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta
     9961 atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg
    10021 actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc
    10081 cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct
    10141 ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat
    10201 attctgtaac ctttataagt aggcataaca gttataatca taacatactg ttttttctta
    10261 ctccacacag gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta
    10321 gctttttaat ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag
    10381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
    10441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
    10501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
    10621 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
    10681 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
    10741 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat
    10801 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
    10861 ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
    10921 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
    10981 caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt
    11041 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
    11101 gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc
    11161 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc
    11221 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11281 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11341 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11401 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    11461 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    11521 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    11581 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
    11641 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
    11701 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
    11761 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
    11821 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
    11881 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
    11941 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    12001 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    12061 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    12121 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    12181 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    12241 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    12301 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    12361 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
//
