LOCUS       sequence-264974-        3739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of sRGECO in cells expressing Flp 
            AND NOT Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-Ef1a-Coff/Fon-sRGECO
FEATURES             Location/Qualifiers
     source          1..3739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        87..1264
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          318..1255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1212..1232
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    complement(1271..1304)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1355..1388)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      1395..1404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1416..1472
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     protein_bind    1767..1800
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1851..1884
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    1891..1924
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(2484..2503)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2999..3032)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    3141..3729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3194..3214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3612..3623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
       61 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
      121 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
      181 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
      241 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
      301 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
      361 gttatggccc ttgcgtgcct tgaattactt ccactggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgaggatcc ataacttcgt ataggatact ttatacgaag ttatgtagct ggagcaattt
     1321 gaaaagattg ttgtattacc tctagctgcg cttaataact tcgtatagta taccttatac
     1381 gaagttatgt cgacgccacc atggtggact catcacgtcg taagtggaat aaggcaggtc
     1441 acgcagtcag agctataggt cggctgagct cacccgtggt ttccgagcgg atgtaccccg
     1501 aggacggcgc cctgaagagc gagatcaaga aggggctgag gctgaaggac ggcggccact
     1561 acgccgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     1621 acatcgtgga catcaagttg gacatcgtgt cccacaacga ggactacacc atcgtggaac
     1681 agtgcgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggag
     1741 gtacaggtga gtacaggagg gctagcataa cttcgtataa agtatcctat acgaagttat
     1801 ttgccttaac ccagaaatta tcactgttat tctttagata ggtgcaaaga ataacttcgt
     1861 ataaggtata ctatacgaag ttatggtacc gaagttccta ttcttcaaat agtataggaa
     1921 cttcaattgt tattggtgca aagaatcact tccttaagtc tttatactgc ccaggaagtt
     1981 cctattcttc aaaaggtata ggaacttcgg cgcgccctac ttcgctgtca tcatttgtac
     2041 aaactcttcg tagtttacct gaccatcccc atcgatgtct gctaccctga tcatttcatc
     2101 aacctcctca tctgttaact tctctccaag gtctgtcatc acgtggcgaa gctctgctgc
     2161 gccgatgtag ccattgccgt ccttatcaaa cacgcggaac gcttcgcgaa tttcctcttc
     2221 actgtctgtg tcattcattt ttcttgccat catcgtcagg aactcaggga agtcgaatgt
     2281 gccgtcaccg tcggcatcta cttcattgat catgtcctgc agctctgctt ctgtggggtt
     2341 ctgccccagc gatcggaaca ccgtcccaag ctccttggtt gttatcgtcc catccccgtc
     2401 cttgtcaaat agggagaaag cctctttaaa ttctgcgatc tgctcttcag tcaggtcgtc
     2461 acgcgtagcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg gggggaagtt
     2521 ggtgccgcgc agcttcacct tgtagatgaa tacgccgtcc tgcagggagg agtcctggtt
     2581 cacgtgaata atgccgccgt cctcgaagtt catcacgcgg tcccacctga agccctcggg
     2641 gaaggacagc ttgaagtagt cggggatgtc ggctgggtgc ttaatgtagg ccttggagcc
     2701 gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac ccttggtcac
     2761 cttcagctta gcggtctgaa aggcctcgta ggggcggccc tcgccctcgc cctcgatctc
     2821 gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga actccttgat
     2881 gatggccatg ttatcctcct cgcccttgct caccagactc ccgcctgtgg gacgacatga
     2941 cttaaccagt taaccatgtt ctctgaacat gagaagggct ggccactctc catctagaga
     3001 agttcctata ctatttgaag aataggaact tcttgcctta acccagaaat tatcactgtt
     3061 attctttaga atagtgcaaa gagaagttcc tatacctttt gaagaatagg aacttcgaat
     3121 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3541 cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg acgtccttct
     3601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3721 cctccccgca tcgataccg
//
