LOCUS       sequence-264995-        3534 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of Arch3.3-EYFP in cells expressing 
            Cre AND NOT Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP
FEATURES             Location/Qualifiers
     source          1..3534
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(981..1014)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1065..1098)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     1211..1232
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1275..1331)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    2011..2044
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    2095..2128
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(2350..2369)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2509..2760
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2697..2718
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2794..2827)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    2936..3524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2989..3009)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3407..3418)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgaagttcc tattctctag aaagtatagg aacttcgcag agtagtagct
      601 ggattgtagc tgctattagc aatctaaaac ctcttagaag ttcctattct tcaaaaggta
      661 taggaacttc ggcgcgccgc caccatggac cccatcgctc tgcaggctgg ttacgacctg
      721 ctgggtgacg gcagacctga aactctgtgg ctgggcatcg gcactctgct gatgctgatt
      781 ggaaccttct actttctggt ccgcggatgg ggagtcaccg ataaggatgc ccgggaatat
      841 tacgctgtga ctatcctggt gcccggaatc gcatccgccg catatctgtc tatgttcttt
      901 ggtatcgggc ttactgaggt gaccgtcggg ggcgaaatgt tggatatcta ttatgccagg
      961 taagtgtcgg ggttgagctc ataacttcgt atagtatacc ttatacgaag ttattctttg
     1021 caccattcta aagaataaca gtgataattt ctgggttaag gcaaataact tcgtatagga
     1081 tactttatac gaagttatgg tacccctcct gtactcacct tgccggtggt gcagatgaac
     1141 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     1201 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     1261 tcgcccttgc tcacgggacc ggggttttct tccacgtctc ctgcttgctt taacagagag
     1321 aagttcgtgg ctccggagcc gggcgcgccc acgttgatgt cgatctggtc cagggggatg
     1381 tactcgccct cgctggtgat cctgctcttg tcggcggcac tgacatcggc accggcactg
     1441 ggttctggtg cctcggtgtc gcccagaata gcccgggatc tcaacaggat aaagccaaag
     1501 ccgaccttgg cagtcacgtc caacaccata aacagcagag tttcgatgcc caggcccacc
     1561 acgccagcgc cctcagtgcc tatgatccac aggatagggt aagcggtcca cagcaccaag
     1621 accagagctg tcagggtgtt aaaggtagat gccacctcgg ggccccgctc ctttgcagca
     1681 gatcgcaggg atgtagccag aaaatagagc accactatca tgcaaattgt agagaacaac
     1741 caccaactgt atctggctat ggccgtgtgg ctcaaggctc cgatgaggcc agtgacgatc
     1801 atcagggcgt ccacacccac cagggtgccg atggtcaccc gatccacctt agcgagaagg
     1861 gccagatcca gcagcagaag tggggtggta aacagccagt cggcgtacct aaacacatga
     1921 gaaatagagt cataagcaca tgggtcacat acaggagatt gtatataaca ttaatacaat
     1981 tttataaaaa aaaggggggg cacaactagt ataacttcgt ataaggtata ctatacgaag
     2041 ttattaagag gtttcatatt gctaatagca gctacaatcc agctaccatt ctgcataact
     2101 tcgtataaag tatcctatac gaagttatgt cgactggaga gtggccagcc cttctcatgt
     2161 tcagagaaca tggttaactg gttaagtcat gtcgtcccac agctgcccgt gccctggccc
     2221 accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg
     2281 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2341 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2401 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2461 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2521 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2581 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2641 cactacctga gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2701 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2761 ttctgctacg agaacgaggt gtaatgagct agcgaagttc ctatactttc tagagaatag
     2821 gaacttctaa gcagacaatc ttgtaataca tcagcagcta gtagattgct ccagctagaa
     2881 gttcctatac cttttgaaga ataggaactt cgaattcgat atcaagctta tcgataatca
     2941 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3001 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3061 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3121 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3181 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3241 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3301 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3361 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3421 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3481 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accg
//
