LOCUS       sequence-265002-        3598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intersectional viral expression of ic++-EYFP in cells expressing Cre
            AND NOT Flp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M,
            Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K
  TITLE     Comprehensive Dual- and Triple-Feature Intersectional Single-Vector 
            Delivery of Diverse Functional Payloads to Cells of Behaving 
            Mammals.
  JOURNAL   Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003.
  PUBMED    32574559
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 
            10.1016/j.neuron.2020.06.003."
COMMENT     Sequence Label: pAAV-nEF-Con/Foff 2.0-iC++-EYFP
FEATURES             Location/Qualifiers
     source          1..3598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..244
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             257..525
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    553..586
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1216..1249)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(1300..1333)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     1446..1467
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    2099..2132
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    2183..2216
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(2438..2457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2597..2848
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     2785..2806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2858..2891)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    3000..3588
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3053..3073)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3471..3482)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
       61 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
      121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
      181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
      241 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
      301 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
      361 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
      421 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
      481 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
      541 gcctacggat ccgaagttcc tattctctag aaagtatagg aacttcgcag agtagtagct
      601 ggattgtagc tgctattagc aatctaaaac ctcttagaag ttcctattct tcaaaaggta
      661 taggaacttc ggcgcgccgc caccatggac tatggcggcg ctttgtctgc cgtcggactc
      721 ttccaaacct catacactct tgagaacaat ggttctgtta tttgcatccc gaacaacggc
      781 cagtgcttct gcttggcttg gcttaaatcc aacggaacaa atgccgagaa gttggctgcc
      841 aacattctgc agtggatttc ttttgcgctt tcagcgctct gcctgatgtt ctacggctac
      901 cagacctgga agtctacttg cggctgggag aatatttacg tggccacgat ccagatgatc
      961 aagttcatca tcgagtattt ccatagcttt gacgaacctg cggtgatcta ctcatccaac
     1021 ggcaacaaga ccaggtggct tcgttacgcg agctggctgc tgacctgccc tgtcattctt
     1081 atccacctga gcaaccttac gggtctggcg aacgactata acaagcgtac catgggtctg
     1141 ctggtgtcag atatcggcac gatcgtgtgg ggcaccacgg ccgcgctgtc caaggtaagt
     1201 gtcggggttg agctcataac ttcgtatagt ataccttata cgaagttatt ctttgcacca
     1261 ttctaaagaa taacagtgat aatttctggg ttaaggcaaa taacttcgta taggatactt
     1321 tatacgaagt tatggtaccc ctcctgtact caccttgccg gtggtgcaga tgaacttcag
     1381 ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga acttgtggcc
     1441 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcctcgcc
     1501 cttgctcacc acgttgatgt cgatctggtc cagggggatg tactcgccct cgctggtgat
     1561 cctgctcttg gcggccgcga ccgcgccagc ctcggcctcg tcctccacca gcgtctcgac
     1621 ctcaatctca gtgccaccaa tgttcaattt ggtggtcttg cgaatgtcgc cgtggatgag
     1681 gatatggctg tggatcagca cgcgcaggta gtggccgagc agaccccagc actgcttcga
     1741 catcaggtca atgatggtgt ggccgacgtt ggagccgtac ctgctcagga cgccgaagcc
     1801 ctcggggccg aggatgaaca ggatggggaa cataccccat gatacgaaga agagccaagc
     1861 catgccagtc accacctggc gacaccggcc cttcggcacg gtgtggtacg cctcaatgta
     1921 gaccttggct gcgttgaaga atgtgtagat gccgtagcac aggcccatca ggaagaaaat
     1981 gacacggacg tatccctaaa cacatgagaa atagagtcat aagcacatgg gtcacataca
     2041 ggagatatgt atataacatt aatacaattt tataaaaaaa agggggggca caactagtat
     2101 aacttcgtat aaggtatact atacgaagtt attaagaggt ttcatattgc taatagcagc
     2161 tacaatccag ctaccattct gcataacttc gtataaagta tcctatacga agttatgtcg
     2221 actggagagt ggccagccct tctcatgttc agagaacatg gttaactggt taagtcatgt
     2281 cgtcccacag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     2341 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2581 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2641 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     2761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2821 cactctcggc atggacgagc tgtacaagta agctagcgaa gttcctatac tttctagaga
     2881 ataggaactt ctaagcagac aatcttgtaa tacatcagca gctagtagat tgctccagct
     2941 agaagttcct ataccttttg aagaatagga acttcgaatt cgatatcaag cttatcgata
     3001 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3061 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3121 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3181 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3241 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3301 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3361 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3421 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3481 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3541 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccg
//
