LOCUS       sequence-26536-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   McCall CM, Miliani de Marval PL, Chastain PD, Jackson SC, He YJ, 
            Kotake Y, Cook JG, Xiong Y
  TITLE     Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a 
            WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is 
            essential for DNA replication and embryonic development.
  JOURNAL   Mol Cell Biol. 2008 Sep . 28(18):5621-33.
  PUBMED    18606781
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Sep . 28(18):5621-33."
COMMENT     Sequence Label: pcDNA3-HA2-Cul4A-deltaROC
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             83..109
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             119..145
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagcta tgtacccata cgatgttcct gactatgcgg gctatcccta
      121 tgacgtcccg gactatgcaa gcttggtacc gttaacatgg atcccccggg ctgcaggaat
      181 tcggcacgag ccagccatgg cggacgaggc cccgcggaag ggcagcttct cggcgctcgt
      241 gggccgcacc aacggcctca ccaagcccgc ggccctggcc gccgcgcccg ccaagccggg
      301 gggcgcgggc ggctccaaga agctggtcat caagaacttc cgagacagac ctcggctgcc
      361 cgacaactac acgcaggaca cgtggcggaa gctgcacgag gcggtgcggg ccgtgcagag
      421 cagcacctcc atcaggtaca acctcgagga gctctaccag gctgtggaaa atctctgttc
      481 tcacaaagtc tccccaatgc tctacaagca actgcgtcag gcctgtgaag accacgtcca
      541 ggcacagatc cttccgttta gagaagactc actagatagt gttttatttt taaagaanat
      601 taacacgtgc tggcangacc actgcagaca aatgatcatg atcagaanca tcttcctgtt
      661 cttgnnncgc anctatgtgc tgcanaactc cacgctgncc tccatctggg atatgggatn
      721 ngnnctgnt
//
