LOCUS       sequence-26552-e         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Ohh M, Park CW, Ivan M, Hoffman MA, Kim TY, Huang LE, Pavletich N, 
            Chau V, Kaelin WG
  TITLE     Ubiquitination of hypoxia-inducible factor requires direct binding 
            to the beta-domain of the von Hippel-Lindau protein.
  JOURNAL   Nat Cell Biol. 2000 Jul . 2(7):423-7.
  PUBMED    10878807
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2000 Jul . 2(7):423-7."
COMMENT     Sequence Label: HA-Elongin B-pcDNA3.1(+)-Zeo
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             116..142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(580..597)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     gap             634..645
                     /estimated_length=12
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagcgtttaa acttaagctt accatggcct acccctacga
      121 cgtgcccgac tacgcctccc tcggatccgc caccatggac gtgtttctca tgatccggcg
      181 ccacaagacc accatcttca cggacgccaa ggagtccagc acggtgttcg aactgaagcg
      241 catcgtcgag ggcatcctca agcggcctcc tgacgagcag cggctgtaca aggatgacca
      301 actcttggat gatggcaaga cactgggcga gtgtggcttc accagtcaaa cagcacggcc
      361 acaggcccca gccacagtgg ggctggcctt ccgggcagat gacaccttcg aggccctgtg
      421 catcgagccg ttttccagcc cgccagagct gcccgatgtg atgaagcccc aggactcggg
      481 aagcagtgcc aatgaacaag ctgtgcagtg aattctgcag atatccatca cactggcggc
      541 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
      601 gccagccatc tgttgtttgc ccctcccccg tgcnnnnnnn nnnnnggnnn nnnncnnntc
      661 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgant angtgtcatt
      721 ctattctggg gggtggggtg gggcangaca gcaaggggga ggantgggaa gacaatagc
//
