LOCUS       sequence-265909-       10612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long 
            isoform.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10612)
  AUTHORS   Edwards DS, Maganti R, Tanksley JP, Luo J, Park JJH, 
            Balkanska-Sinclair E, Ling J, Floyd SR
  TITLE     BRD4 Prevents R-Loop Formation and Transcription-Replication 
            Conflicts by Ensuring Efficient Transcription Elongation.
  JOURNAL   Cell Rep. 2020 Sep 22;32(12):108166. doi: 
            10.1016/j.celrep.2020.108166.
  PUBMED    32966794
REFERENCE   2  (bases 1 to 10612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-09-22"; volume: "32(12):108166. doi"; pages: " 
            10.1016/j.celrep.2020.108166"
COMMENT     Sequence Label: pCW57-mCherry-2A-BRD4 Iso A
FEATURES             Location/Qualifiers
     source          1..10612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     188..208
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        190..208
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        236..462
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             463..643
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    690..815
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1308..1541
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1726..1770
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2100
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2154..2468
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2162..2180
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2198..2216
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2233..2251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2269..2287
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2305..2323
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2340..2358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2376..2394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2441..2465
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2501..2534)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2537..3244
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2682..2700)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(3245..3277)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3287..3340
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3392..3415
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5633..5650
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7871..7882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8087..8320
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8392..8513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8429..8448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8483..8502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8553..8688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8688..8759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8760..8828,8829..9620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8978..8997)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9791..10379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10280..10299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10533..10550
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      181 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      241 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      301 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      361 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      421 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      481 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      541 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      601 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      661 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      721 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      781 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      841 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
      901 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
      961 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1021 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1081 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1141 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1201 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1261 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1321 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1381 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1441 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1501 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1561 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1621 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1681 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1741 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1801 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1861 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1921 cacctcccaa ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac
     1981 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     2101 cgggtttatt acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta
     2161 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga
     2221 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     2281 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat
     2341 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat
     2401 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2461 agatcgcctg gagaattggc tagcgctacc ggtcgccacc ataacttcgt atagcataca
     2521 ttatacgaag ttattaatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     2581 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     2641 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     2701 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     2761 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     2821 cttcaattgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     2881 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     2941 ctccgacggc cccgtaatgc agtgtcgtac catgggctgg gaggcctcca ctgagcggat
     3001 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     3061 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     3121 cggcgcctac aacgtcgaca tcaagttgga catcctttcc cacaacgagg actacaccat
     3181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagcttta
     3241 taagataact tcgtatagca tacattatac gaagttaggt tctggtgaag gtagaggttc
     3301 tctcctcact tgtggtgatg ttgaagaaaa ccctggtcca cgaacgcgtt ctgacactat
     3361 gtacaagtcc ggactcagat ctcgagcgat ggattacaag gatgacgacg ataagggatc
     3421 tatgtctgcg gagagcggcc ctgggacgag attgagaaat ctgccagtaa tgggggatgg
     3481 actagaaact tcccaaatgt ctacaacaca ggcccaggcc caaccccagc cagccaacgc
     3541 agccagcacc aaccccccgc ccccagagac ctccaaccct aacaagccca agaggcagac
     3601 caaccaactg caatacctgc tcagagtggt gctcaagaca ctatggaaac accagtttgc
     3661 atggcctttc cagcagcctg tggatgccgt caagctgaac ctccctgatt actacaaaat
     3721 tatcaagact ccgatggaca tgggaacaat aaagaagcgc ttggaaaaca actattactg
     3781 gaatgctcag gaatgtatcc aggacttcaa cactatgttt acaaattgtt acatctacaa
     3841 caagcctgga gatgacatag tcttaatggc agaagctctg gaaaagctct tcttgcaaaa
     3901 aataaatgag ctacccacag aagagacaga aataatgatc gtacaagcaa aaggaagagg
     3961 acgtgggagg aaagaaacag ggacagcaaa acctggcgtt tccacggtac caaacacaac
     4021 tcaagcatcg actcctccgc agacccagac ccctcagccg aatcctcctc ctgtgcaggc
     4081 cacgcctcac cccttccctg ccgtcacccc ggacctcatc gtccagaccc ctgtcatgac
     4141 agtggtgcct ccccagccac tgcagacgcc cccgccagtg cccccccagc cacaaccccc
     4201 acccgctcca gctccccagc ccgtacagag ccacccaccc atcatcgcgg ccaccccaca
     4261 gcctgtgaag acaaagaagg gagtgaagag gaaagcagac accaccaccc ccaccaccat
     4321 tgaccccatt cacgagccac cctcgctgcc cccggagccc aagaccacca agctgggcca
     4381 gcggcgggag agcagccggc ctgtgaaacc tccaaagaag gacgtgcccg actctcagca
     4441 gcacccagca ccagagaaga gcagcaaggt ctcggagcag ctcaagtgct gcagcggcat
     4501 cctcaaggag atgtttgcca agaagcacgc cgcctacgcc tggcccttct acaagcctgt
     4561 ggacgtggag gcactgggcc tacacgacta ctgtgacatc atcaagcacc ccatggacat
     4621 gagcacaatc aagtctaaac tggaggcccg tgagtaccgt gatgctcagg agtttggtgc
     4681 tgacgtccga ttgatgttct ccaactgcta taagtacaac cctcctgacc atgaggtggt
     4741 ggccatggcc cgcaagctcc aggatgtgtt cgaaatgcgc tttgccaaga tgccggacga
     4801 gcctgaggag ccagtggtgg ccgtgtcctc cccggcagtg ccccctccca ccaaggttgt
     4861 ggccccgccc tcatccagcg acagcagcag cgatagctcc tcggacagtg acagttcgac
     4921 tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc agctcaaagc
     4981 cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa agaaaaagga
     5041 gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag tggaagagaa
     5101 taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata atagcagcaa
     5161 cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc ccacgtatga
     5221 gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc agctcagctt
     5281 ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca tccagtcacg
     5341 ggagccctcc ctgaagaatt ccaaccccga cgagattgaa atcgactttg agaccctgaa
     5401 gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc tgtttgcgga agaaaaggaa
     5461 acctcaagct gagaaagttg atgtgattgc cggctcctcc aagatgaagg gcttctcgtc
     5521 ctcagagtcg gagagctcca gtgagtccag ctcctctgac agcgaagact ccgaaacaga
     5581 gatggctccg aagtcaaaaa agaaggggca ccccgggagg gagcagaaga agcaccatca
     5641 tcaccaccat cagcagatgc agcaggcccc ggctcctgtg ccccagcagc cgcccccgcc
     5701 tccccagcag cccccaccgc ctccacctcc gcagcagcaa cagcagccgc cacccccgcc
     5761 tcccccaccc tccatgccgc agcaggcagc cccggcgatg aagtcctcgc ccccaccctt
     5821 cattgccacc caggtgcccg tcctggagcc ccagctccca ggcagcgtct ttgaccccat
     5881 cggccacttc acccagccca tcctgcacct gccgcagcct gagctgcccc ctcacctgcc
     5941 ccagccgcct gagcacagca ctccacccca tctcaaccag cacgcagtgg tctctcctcc
     6001 agctttgcac aacgcactac cccagcagcc atcacggccc agcaaccgag ccgctgccct
     6061 gcctcccaag cccgcccggc ccccagccgt gtcaccagcc ttgacccaaa cacccctgct
     6121 cccacagccc cccatggccc aaccccccca agtgctgctg gaggatgaag agccacctgc
     6181 cccacccctc acctccatgc agatgcagct gtacctgcag cagctgcaga aggtgcagcc
     6241 ccctacgccg ctactccctt ccgtgaaggt gcagtcccag cccccacccc ccctgccgcc
     6301 cccaccccac ccctctgtgc agcagcagct gcagcagcag ccgccaccac ccccaccacc
     6361 ccagccccag cctccacccc agcagcagca tcagccccct ccacggcccg tgcacttgca
     6421 gcccatgcag ttttccaccc acatccaaca gcccccgcca ccccagggcc agcagccccc
     6481 ccatccgccc ccaggccagc agccaccccc gccgcagcct gccaagcctc agcaagtcat
     6541 ccagcaccac cattcacccc ggcaccacaa gtcggacccc tactcaaccg gtcacctccg
     6601 cgaagccccc tccccgctta tgatacattc cccccagatg tcacagttcc agagcctgac
     6661 ccaccagtct ccaccccagc aaaacgtcca gcctaagaaa caggagctgc gtgctgcctc
     6721 cgtggtccag ccccagcccc tcgtggtggt gaaggaggag aagatccact cacccatcat
     6781 ccgcagcgag cccttcagcc cctcgctgcg gccggagccc cccaagcacc cggagagcat
     6841 caaggccccc gtccacctgc cccagcggcc ggaaatgaag cctgtggatg tcgggaggcc
     6901 tgtgatccgg cccccagagc agaacgcacc gccaccaggg gcccctgaca aggacaaaca
     6961 gaaacaggag ccgaagactc cagttgcgcc caaaaaggac ctgaaaatca agaacatggg
     7021 ctcctgggcc agcctagtgc agaagcatcc gaccaccccc tcctccacag ccaagtcatc
     7081 cagcgacagc ttcgagcagt tccgccgcgc cgctcgggag aaagaggagc gtgagaaggc
     7141 cctgaaggct caggccgagc acgctgagaa ggagaaggag cggctgcggc aggagcgcat
     7201 gaggagccga gaggacgagg atgcgctgga gcaggcccgg cgggcccatg aggaggcacg
     7261 tcggcgccag gagcagcagc agcagcagcg ccaggagcaa cagcagcagc agcaacagca
     7321 agcagctgcg gtggctgccg ccgccacccc acaggcccag agctcccagc cccagtccat
     7381 gctggaccag cagagggagt tggcccggaa gcgggagcag gagcgaagac gccgggaagc
     7441 catggcagct accattgaca tgaatttcca gagtgatcta ttgtcaatat ttgaagaaaa
     7501 tcttttctga gcgcacctag gtggcttcaa gcttcgaatt ctgcagtcga cggtaccgcg
     7561 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     7621 gttttacttg ctttaaaaaa cctcccacac ctccccctga acccagctcc tttccgggac
     7681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     7741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     7801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     7861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     7921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     7981 ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac ctttaagacc aatgacttac
     8041 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     8101 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     8161 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     8221 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     8281 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     8341 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     8401 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8461 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8521 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8581 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8641 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggcctt tcaaatatgt
     8701 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8761 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8821 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8881 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8941 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9001 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9061 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9121 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9181 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9241 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9301 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9361 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9421 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9481 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9541 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9601 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9661 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9721 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9781 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9841 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9901 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9961 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10021 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10081 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10141 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10201 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10261 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10321 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10381 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10441 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10501 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10561 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tg
//
