LOCUS       sequence-265959-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for expression of roGFP2 and HyPer Red.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Faber SC, McNabb NA, Ariel P, Aungst ER, McCullough SD
  TITLE     Exposure Effects Beyond the Epithelial Barrier: Trans-Epithelial 
            Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung
            Fibroblasts in an Organotypic Human Airway Model.
  JOURNAL   Toxicol Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085.
  PUBMED    32525552
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicol 
            Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085."
COMMENT     Sequence Label: pLV-dfROX
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..87
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ctcggcatgg ncgagctgta caagggaagc ggagagggca gaggaagtct gctaacatgc
       61 ggtgacgtcg aggagaatcc tggacccatg gagatggcga gccagcaggg cgagacgatg
      121 tccggaccgc tgcacattgg tttgattccc acagttggac cgtacctgct accgcatatt
      181 atccctatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
      241 caccagttac tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcactggtg
      301 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
      361 tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa
      421 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatgcc ccgcacggtt
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg
      541 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      661 gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagggagg tacaggcggg agtctggtga gcaagggcga ggaggataac
      781 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      841 cacgagttcg agatcgaggg cgagggcgan ggccgcccct acgaggcctt tcanaccgct
      901 aagctgaagg tgaccanggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      961 ttcatgtacg gctccaaggc ctacattaag cacccagccg acntccccga ctacttcaag
     1021 ctgtccttcc ccgagggctt cngnngggag cgcgngatga acttcnngnn ggnnnattat
     1081 tcncntnanc nngnctcctn ccnncngnng ngtantcatn nannnnnanc tnnnnnncnn
     1141 nncccccgan nnnccnnnan nnnnnanacn tgggcngggn ng
//
