LOCUS       sequence-265988-       10727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Kim J, Kato M, Beachy PA
  TITLE     Gli2 trafficking links Hedgehog-dependent activation of Smoothened 
            in the primary cilium to transcriptional activation in the nucleus.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21666-71. Epub 2009 
            Dec 8.
  PUBMED    19996169
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2009-12-22"; volume: "106(51)"; pages: 
            "21666-71. Epub 2009 Dec 8"
COMMENT     Sequence Label: pCEFL3xHAmGli2
FEATURES             Location/Qualifiers
     source          1..10727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          213..1155
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1112..1132
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1184..1193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1196..1222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1226..1252
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1259..1285
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(5964..5982)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5965..5982)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6002..6019)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6008..6232
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6278..6706
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6365..6384)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6575..6596
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6715..6735)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6720..7049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6900..7035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7116..7910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7170..7189)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7780..7799
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8084..8205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8121..8140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8175..8194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8254..8270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8254..8270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8267..8289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8278..8294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8302..8308,8309..8326,8327..8332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8347..8368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8485..8502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8656..9244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8736..8755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9415..10206,10207..10275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10038..10057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10276..10380)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10459..10478)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(10709..10727,1..1164)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
       61 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
      121 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
      181 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
      241 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
      301 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
      361 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
      421 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
      481 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
      541 cgggccaaga tcgatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      601 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      661 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      721 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      781 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
      841 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
      901 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
      961 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1021 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1081 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1141 tttcttccat ttcaggtgtc gtgaggaatt ggcgaagctt gccgccacca tgggataccc
     1201 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg ctgctagcta
     1261 tccatatgac gttccagatt acgctagatc cactagtgag acttctgccc cagcccctgc
     1321 actggagaag aaagaagcca agagtggtct cttggaggac agcagcttcc ccgacccagg
     1381 gaaaaaggcc tgtcctctgg cggtggccgc agctgtagcc gcccacggag ttcctcagca
     1441 gctcctgccg gctttccacg cgcctttgcc gattgacatg agacaccagg agggaaggta
     1501 ccattatgac cctcactctg tccacagtgt acacgggcct cccaccctaa gtggcagccc
     1561 tgtcatctca gatatctcct tgatacgact ttctccacac cctgctggcc ctggagagtc
     1621 acccttcagc gcccaccacc cctacgtgaa cccccatatg gagcactacc tccggtctgt
     1681 gcacagcagc cccacactct caatgatctc tgccgccagg ggcctcagcc ctgctgatgt
     1741 ggcccacgaa catctgaaag agaggggact ctttagcctc gcagccccag gcaccaaccc
     1801 ttcagactat taccaccaga tgaccctcat ggcaagccac cccacccctt atggggacct
     1861 tctaatgcag agcgggggtg ctgctagcgc accccatctc catgactacc tcaaccctgt
     1921 ggatgcatca cgattctcta gtccacgtgt gaccccacga ctgagccgca agcgggctct
     1981 gtccatctcc ccgctctcag atgccagcct cgacctacaa cgcatgattc ggacctctcc
     2041 caactcgctg gtagcttaca tcaacaactc caggagcagc tcagcagcca gtggctctta
     2101 tggacatctg tctgctggtg ccctcagccc agccttcact tttccccacc ccatcaatcc
     2161 tgtggcctac cagcagatct tgagccagca gcggggcctg ggctcagcct ttggacacac
     2221 accacccctg atccagcctt cacccacctt cttggcccag cagcccatga ctctcacctc
     2281 catcagcacc atgcctaccc aactcagcag cagtagcagc aactgtctaa atgatgccaa
     2341 ccagaacaag cagaacagcg agtcagctgt gagcagcacc gtgaacccca tcaccattca
     2401 taagcggagc aaggtcaaga ctgaggctga gggcctgcgt ccagcatccc cgcttggact
     2461 gacacaggag cagctggccg atctcaagga agacctggac agggatgact gtaagcagga
     2521 ggccgaggtg gtcatctacg agaccaactg ccactgggca gactgcacca aggagtatga
     2581 cacacaggag cagctggtgc atcatatcaa caatgaacac atccacgggg agaagaagga
     2641 gttcgtgtgc cgctggcagg cctgcacgag agagcagaag cccttcaagg cccagtacat
     2701 gctggttgtt cacatgcgca gacacacggg tgagaagcca cacaagtgca cgttcgaagg
     2761 ctgttccaag gcctactctc gcctggagaa cctgaagaca cacctgcgtt cacacacagg
     2821 agagaagcca tatgtgtgtg aacacgaagg ctgtaacaaa gccttctcca atgcctcaga
     2881 ccgcgccaag caccagaacc gcactcactc caatgagaaa ccctacatct gcaagatccc
     2941 aggctgcacc aagaggtaca cagaccccag ctcactccgc aagcatgtga agactgtcca
     3001 tgggccagac gcccatgtca ccaagaaaca gcgtaatgat gtgcatgtcc gtgctccact
     3061 gctcaaggag aatggggata atgaggccag cgccgagcca ggtggccggg gacctgagga
     3121 gagtgtggag gccagtagca ccagccacac tgtggaggac tgcctacata tcaaagccat
     3181 caagacagag agctccgggc tttgtcagtc cagccccggg gcccagtcat cctgcagcag
     3241 cgagccctct cccctgggca gtgcccccaa caatgacagt ggcatggaga tgccggggac
     3301 agggcctggg agtctgggag acctgacagc actggctgac acgtgtccag gagctgacac
     3361 ctcagccctg gctgcaccct ccactggtgg cctgcagctg cgcaaacaca tgagcaccgt
     3421 gcatcgcttt gagcagctga agagagagaa gctcaagtca ctgaaggatt cctgctcgtg
     3481 ggccggccca gctccacaca cccgcaacac caagctgcct ccccttccag tcaatggttc
     3541 tgtcctggaa aacttcaaca atacaggggg cggtggaccg gcaggactgc tgcccagcca
     3601 gcggctacca gagctgaccg aagtgacgat gctgagccag ctgcaggaac gaagagacag
     3661 ctccaccagc accatgagct cggcctacac tgtgagccgc cgctcctctg gcatctcccc
     3721 atacttctct agccgtcgct ccagcgaggc ttcgcctctc ggtggcctac gcccgcacaa
     3781 cgccagctca gcagactcct atgaccccat ctccacagat gcctctcggc gctccagtga
     3841 agccagccag tgcagtggcg gtggcccaga gctgctcaac ctcacacctg cgcagcagta
     3901 caacctgcgt gccaagtacg cagcggccac aggtggacca ccgcccacgc cactgccggg
     3961 cctcgatcgt gtaagccttc gtacccgcct ggccttgctg gatgctcctg agcgtgcact
     4021 tcctggtgcc tgcccacatc cactggggcc acggcgtggc agcgatgggc ctacctatag
     4081 ccatggtcat ggccatggct acgcaggtgc ggctccagca ttcccccacg aggggccaaa
     4141 cagcagcaca cggcgggcca gcgaccctgt gcggcgccct gaccccctta ttctgcctcg
     4201 agtgcaacgt ttccacagta cccacaacat gaatccaggt tcactgccac cctgcgctga
     4261 tcggcgtggc ctgcacgtac agagccaccc cagcgtagac agcaacctga cccgcaacgc
     4321 ctactctccc agacccccta gcatcaatga gaacgtggtg atggaggccg tggctgctgg
     4381 ggtagacggc ccagggctag agtgcgacct ggggctggtg gaggatgagc tggtgctgcc
     4441 agatgatgtg gtacagtaca tcaaggctca caccggtggt accttggatg acggcattcg
     4501 gcaggggtat cccacagaag gtactggctt ccccgagaac tctaagctgc ccagtcctgg
     4561 gctacaaggc caccgcaggc tagcagctgc cgactccaac atgggtcctt ctgctcctgg
     4621 actcgggggc tgccagctga gctacagccc ctcctccaac ctcaacaaga gcaacatgcc
     4681 tgtgcagtgg aatgaggtga gttctggcac cgtggatgcc ctgcctaccc aggtgaagcc
     4741 acctcctttc cctcacagca acctggctgt ggtccaacag aagccagcct ttggccagta
     4801 tccaggatat aatccacaat ccgtgcagag cagctccgga ggtctagaca gcacccagcc
     4861 gcacctacag cttcgaggag ccccctctgc atcaagaggg agctacacgc aacagcctcg
     4921 acagccagct gcaggcagtc agtgcctggg tatgagtgcg gccatgagcc cgcaggccag
     4981 ctacagccaa gcccaccccc agctgagccc aaacattgtc agcggatctc tgaaccagtt
     5041 ttctccctcc tgcagcaata tggcagccaa gcccagccac ctgggactcc ctcagcaaat
     5101 ggaagttgtc cccaatgcca ccatcatgaa tggccatcaa cgggagcacg gggtccccaa
     5161 ttcatccctg gctgcggtgt cacaacctca cccagtcctg agctatcccc agcaggacag
     5221 ctaccaacag ggctccaacc ttctgtcatc ccatcagcct ggcttcatgg agtcccagca
     5281 gaacgcgggc tttggtctca tgcagcctcg gccacccctg gaacccaaca cggccagccg
     5341 tcaccgtgga gtacgttctg ggcaacagca gttgtatgcc aggaccactg gccaagccat
     5401 ggtcacatca gccaaccaag agacagcaga agctatgccc aagggaccag cagggaccat
     5461 ggtatcccta gctcctcagc catctcagga cacagggcgg gcacaagatc agaacacgct
     5521 atactactat ggccagatcc acatgtatga acagaatgga ggctgcccag ccgtgcagcc
     5581 ccagccgcca caaccacaag cttgctcaga cagtatccag cctgagcctt tgccttcacc
     5641 gggagtcaac caggtgtcta gcaccgtgga ctcccagctc ctggagcccc cccagattga
     5701 ctttgatgcc atcatggatg atggtgatca ctcgagtttg ttttctggtg cactgagccc
     5761 aacccttctc cacaatctct cccagaattc ctcacgcctc accacacccc ggaattcctt
     5821 gacactgccc tccatccctg cgggcatcag caacatggcc gtgggcgaca tgagttccat
     5881 gctcaccagc ctggctgaag agagcaagtt tttaaacatg atgacctaag cggccgctcg
     5941 agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca
     6001 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6061 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6121 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6181 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6241 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6301 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6361 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6421 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6481 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6541 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6601 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6661 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     6721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6961 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7021 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7081 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7141 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7201 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7261 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7321 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7381 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7441 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7501 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7561 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7621 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7681 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     7741 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     7801 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     7861 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     7921 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     7981 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8041 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8101 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8161 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8221 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8281 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8341 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8401 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8461 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8521 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8581 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8641 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8701 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8761 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8821 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8881 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8941 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9001 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9061 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9121 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9181 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9241 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9301 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9361 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9421 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9481 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9541 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9601 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9661 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9721 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9781 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9841 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9901 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9961 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10021 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10081 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10141 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10201 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10261 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10321 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10381 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat ctcccgatcc
    10441 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    10501 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    10561 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    10621 tcgagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10681 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagt
//
