LOCUS       sequence-266021-        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Visualization of GRK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Pietraszewska-Bogiel A, Joosen L, Chertkova AO, Goedhart J
  TITLE     Not So Dry After All: DRY Mutants of the AT1A Receptor and H1 
            Receptor Can Induce G-Protein-Dependent Signaling.
  JOURNAL   ACS Omega. 2020 Feb 3;5(6):2648-2659. doi: 10.1021/acsomega.9b03146.
            eCollection 2020 Feb 18.
  PUBMED    32095688
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.9b03146"; journalName: "ACS Omega"; date: 
            "2020-02-3- 3"; volume: "5"; issue: "6"; pages: "2648-2659"
COMMENT     Sequence Label: BtGRK2[D110A K220R]-mVenus
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(24..734,735..737,738..740))
                     /codon_start=1
                     /product="monomeric optimized variant of EYFP, identical to
                     mVenus but with L68V (Kremers et al., 2006)"
                     /label=SYFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     415..434
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      737..746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggggacgagt cgcgggcgct ttacttgtac agctcgtcca tgccgagagt gatcccggcg
       61 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
      121 ttggactggt agctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      181 gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
      241 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtggctg
      301 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      361 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      421 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      481 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccg
      541 tagcccaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatc
      601 agcttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      661 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      721 tcctcgccct tgctcaccat ggtggcgacc ggtccgaggc gggtggggag ctcttcctcg
      781 ctgtcgctgc agtttccaca gcaatcttgg cgactgaaga gtctctgcag cagtcccttt
      841 ttaggaggtg caggtggctg gcccttccag tccaggtctg ggggaactga gccatccagc
      901 ccaaagacat tcagctcttg gaagcactcg gtctccacca tctcgttctg ccaggggatg
      961 ggcacactgc ctgtggcaaa cttctggtag aagtcctggt cggtaggctc cagctccacg
     1021 cccttgaccg tagagaactg ttcaatgtcc agaacatcct tgcagtaatg ggcctggggg
     1081 tcaggcttga acggcggctc cagcatgcca gctccagacc gcttgaagtc acttcttaag
     1141 aagggggtgc tccttcacct cgcgggactg ccccacgaca ccccaggcgt tcggcagggt
     1201 ccttgcaaaa gaactttgaa caaaatgagc gggcctgcgg ggaaaaacgc tcggaaaaat
     1261 ccccggggac tcctttccca gccgctcact tcctccgctt gatttctttt tccttggggg
     1321 aaaggggaag gggcggggat tttttgtaag ggggggccaa gccccccctt gggggggagg
     1381 gtcgtttttt tttcccctcc ggaccgttac cggggcgccc ccccttttga tgggggccgc
     1441 gccccaccac tcacccccca aaaaaaagga gaggggcggg gtcccccaaa attttgttcg
     1501 cccttaaaac cccggggccc cccaaaaaat cgagagaaac gctcggagac cccctggtag
     1561 aattgccccc tctccccccc ctctgccaca aaacaatttt tcccccctct tctcttaaag
     1621 cacccgcgaa tattcttctc gagacccccc ccgccgggga gctgggaagg gtcccggggg
     1681 gggggggggc agtacagaag ggagaaaagg gaaa
//
