LOCUS       sequence-266107-        8054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Nekrasova OE, Amargo EV, Smith WO, Chen J, Kreitzer GE, Green KJ
  TITLE     Desmosomal cadherins utilize distinct kinesins for assembly into 
            desmosomes.
  JOURNAL   J Cell Biol. 2011 Dec 26;195(7):1185-203. Epub 2011 Dec 19.
  PUBMED    22184201
REFERENCE   2  (bases 1 to 8054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2011-12-26"; volume: "195(7)"; pages: "1185-203. Epub
            2011 Dec 19"
COMMENT     Sequence Label: Desmoglein2-mCherry
FEATURES             Location/Qualifiers
     source          1..8054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        306..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     460..480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3944..3953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3950..4660
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4095..4113)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4363..4382
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    4783..4904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4820..4839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4874..4893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4911..5366)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5048..5069)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5260..5279
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5393..5497
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5499..5856
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5499..5690
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5522..5542)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5707..5842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5891..6685
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5891..6685
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5945..5964)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6555..6574
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6873..6892)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6917..6964
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7293..7881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7782..7801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
       61 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      481 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct
      541 accggactca gatctcgagg gtgcgatggc gcggagcccg ggacgcgcgt acgccctgct
      601 gcttctcctg atctgcttta acgttggaag tggacttcac ttacaggtct taagcacaag
      661 aaatgaaaat aagctgcttc ctaaacatcc tcatttagtg cggcaaaagc gcgcctggat
      721 caccgccccc gtggctcttc gggagggaga ggatctgtcc aagaagaatc caattgccaa
      781 gatacattct gatcttgcag aagaaagagg actcaaaatt acttacaaat acactggaaa
      841 agggattaca gagccacctt ttggtatatt tgtctttaac aaagatactg gagaactgaa
      901 tgttaccagc attcttgatc gagaagaaac accatttttt ctgctaacag gttacgcttt
      961 ggatgcaaga ggaaacaatg tagagaaacc cttagagcta cgcattaagg ttcttgatat
     1021 caatgacaac gaaccagtgt tcacacagga tgtctttgtt gggtctgttg aagagttgag
     1081 tgcagcacat actcttgtga tgaaaatcaa tgcaacagat gcagatgagc ccaataccct
     1141 gaattcgaaa atttcctata gaatcgtatc tctggagcct gcttatcctc cagtgttcta
     1201 cctaaataaa gatacaggag agatttatac aaccagtgtt accttggaca gagaggaaca
     1261 cagcagctac actttgacag tagaagcaag agatggcaat ggagaagtta cagacaaacc
     1321 tgtaaaacaa gctcaagttc agattcgtat tttggatgtc aatgacaata tacctgtagt
     1381 agaaaataaa gtgcttgaag ggatggttga agaaaatcaa gtcaatgtag aagttacgcg
     1441 cataaaagtg ttcgatgcag atgaaatagg ttctgataat tggctggcaa attttacatt
     1501 tgcatcagga aatgaaggag gttatttcca catagaaaca gatgctcaaa ctaacgaagg
     1561 aattgtgacc cttattaagg aagtagatta tgaagaaatg aagaatcttg acttcagtgt
     1621 tattgtcgct aataaagcag cttttcacaa gtcgattagg agtaaataca agcctacacc
     1681 cattcccatc aaggtcaaag tgaaaaatgt gaaagaaggc attcatttta aaagcagcgt
     1741 catctcaatt tatgttagcg agagcatgga tagatcaagc aaaggccaaa taattggaaa
     1801 ttttcaagct tttgatgagg acactggact accagcccat gcaagatatg taaaattaga
     1861 agatagagat aattggatct ctgtggattc tgtcacatct gaaattaaac ttgcaaaact
     1921 tcctgatttt gaatctagat atgttcaaaa tggcacatac actgtaaaga ttgtggccat
     1981 atcagaagat tatcctagaa aaaccatcac tggcacagtc cttatcaatg ttgaagacat
     2041 caacgacaac tgtcccacac tgatagagcc tgtgcagaca atctgtcacg atgcagagta
     2101 tgtgaatgtt actgcagagg acctggatgg acacccaaac agtggccctt tcagtttctc
     2161 cgtcattgac aaaccacctg gcatggcaga aaaatggaaa atagcacgcc aagaaagtac
     2221 cagtgtgctg ctgcaacaaa gtgagaaaaa gcttgggaga agtgaaattc agttcctgat
     2281 ttcagacaat cagggtttta gttgtcctga aaagcaggtc cttacactca cagtttgtga
     2341 gtgtctgcat ggcagcggct gcagggaagc acagcatgac tcctatgtgg gcctgggacc
     2401 cgcagcaatt gcgctcatga ttttggcctt tctgctcctg ctattggtac cacttttact
     2461 gctgatgtgc cattgcggaa agggcgccaa aggctttacc cccatacctg gcaccataga
     2521 gatgctgcat ccttggaata atgaaggagc accacctgaa gacaaggtgg tgccatcatt
     2581 tctgccagtg gatcaagggg gcagtctagt aggaagaaat ggagtaggag gtatggccaa
     2641 ggaagccacg atgaaaggaa gtagctctgc ttccattgtc aaagggcaac atgagatgtc
     2701 cgagatggat ggaaggtggg aagaacacag aagcctgctt tctggtagag ctacccagtt
     2761 tacaggggcc acaggcgcta tcatgaccac tgaaaccacg aagaccgcaa gggccacagg
     2821 ggcttccaga gacatggccg gagctcaggc agctgctgtt gcactgaacg aagaattctt
     2881 aagaaattat ttcactgata aagcggcctc ttacactgag gaagatgaaa atcacacagc
     2941 caaagattgc cttctggttt attctcagga agaaactgaa tcgctgaatg cttctattgg
     3001 ttgttgcagt tttattgaag gagagctaga tgaccgcttc ttagatgatt tgggacttaa
     3061 attcaagacg ctagctgaag tttgcctggg tcaaaaaata gatataaata aggaaattga
     3121 gcagagacaa aaacctgcca cagaaacaag tatgaacaca gcttcacatt cactctgtga
     3181 gcaaactatg gttaattcag agaataccta ctcctctggc agtagcttcc cagttccaaa
     3241 atctttgcaa gaagccaatg cagagaaagt aactcaggaa atagtcactg aaagatctgt
     3301 gtcttctagg caggcgcaaa aggtagctac acctcttcct gacccaatgg cttctagaaa
     3361 tgtgatagca acagaaactt cctatgtcac agggtccact atgccaccaa ccactgtgat
     3421 cctgggtcct agccagccac agagccttat tgtgacagag agggtgtatg ctccagcttc
     3481 taccttggta gatcagcctt atgctaatga aggtacagtt gtggtcactg aaagagtaat
     3541 acagcctcat gggggtggat cgaatcctct ggaaggcact cagcatcttc aagatgtacc
     3601 ttacgtcatg gtgagggaaa gagagagctt ccttgccccc agctcaggtg tgcagcctac
     3661 tctggccatg cctaatatag cagtaggaca gaatgtgaca gtgacagaaa gagttctagc
     3721 acctgcttcc actctgcaat ccagttacca gattcccact gaaaattcta tgacggctag
     3781 gaacaccacg gtgtctggag ctggagtccc tggccctctg ccagattttg gtttagagga
     3841 atctggtcat tctaattcta ccataaccac atcttccacc agagtcacca agcatagcac
     3901 tgtacagcat tcttactccc cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
     3961 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     4021 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4081 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     4141 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     4201 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4261 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4321 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4381 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4441 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4501 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4561 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4621 ccgccactcc accggcggca tggacgagct gtacaagtaa agcggccgcg actctagatc
     4681 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4741 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4801 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4861 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag
     4921 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4981 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     5041 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     5101 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     5161 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     5221 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     5281 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     5341 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     5401 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5461 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     5521 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5581 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5641 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5701 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5761 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5821 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5881 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5941 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6001 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6061 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6121 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6181 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6241 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6301 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6361 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     6421 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6481 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6541 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6601 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6661 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6721 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6781 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6841 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6901 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6961 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     7021 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     7081 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     7141 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7201 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7261 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7321 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7381 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7441 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7501 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7561 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7621 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7681 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7741 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7801 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7861 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7921 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7981 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     8041 atatatggag ttcc
//
