LOCUS       sequence-266262-       15190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15190)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL079
FEATURES             Location/Qualifiers
     source          1..15190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_RNA        3519..3594
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4100..6484
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6497..6517
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6528..6776
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    6797..6821
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8121..8750
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9184..10251
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      10317..10511
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10747..10769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10855..10995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10910..10929
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11010..11027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11181..11769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11261..11280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11856..12650)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13075..13099
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13177..13351
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(14441..15118)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt aagtttctgc
     3481 ttctaccgct tttgtcaccg gaggagaccg gggtctcagt tttagagcta gaaatagcaa
     3541 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgcttttgt
     3601 caccggatgt gttttccggt ctgatgagtc cgtgaggacg aaacaaaagc tttgatatat
     3661 atataataat tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa
     3721 aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta
     3781 taacttttct aatatatgac caaaatttgt tgatgtgcag gtggtggata agggtgctag
     3841 tgctcagtct ttcatcgaga ggatgaccaa cttcgataag aaccttccaa acgagaaggt
     3901 gctccctaag cactctttgc tctacgagta cttcaccgtg tacaacgagt tgaccaaggt
     3961 taagtacgtg accgagggaa tgaggaagcc tgcttttttg tcaggtgagc aaaagaaggc
     4021 tatcgttgat ctcttgttca agaccaacag aaaggtgacc gtgaagcagc tcaaagagga
     4081 ttacttcaag aaaatcgagt gcttcgattc agttgagatt tctggtgttg aggataggtt
     4141 caacgcatct ctcggaacct accacgatct cctcaagatc attaaggata aggatttctt
     4201 ggataacgag gaaaacgagg atatcttgga ggatatcgtt cttaccctca ccctctttga
     4261 agatagagag atgattgaag aaaggctcaa gacctacgct catctcttcg atgataaggt
     4321 gatgaagcag ttgaagagaa gaagatacac tggttgggga aggctctcaa gaaagctcat
     4381 taacggaatc agggataagc agtctggaaa gacaatcctt gatttcctca agtctgatgg
     4441 attcgctaac agaaacttca tgcagctcat ccacgatgat tctctcacct ttaaagagga
     4501 tatccagaag gctcaggttt caggacaggg tgatagtctc catgagcata tcgctaacct
     4561 cgctggatct cctgcaatca agaagggaat cctccagact gtgaaggttg tggatgagtt
     4621 ggtgaaggtg atgggaaggc ataagcctga gaacatcgtg atcgaaatgg ctagagagaa
     4681 ccagaccact cagaagggac agaagaactc tagggaaagg atgaagagga tcgaggaagg
     4741 tatcaaagag cttggatctc agatcctcaa agagcaccct gttgagaaca ctcagctcca
     4801 gaatgagaag ctctacctct actacctcca gaacggaagg gatatgtatg tggatcaaga
     4861 gttggatatc aacaggctct ctgattacga tgttgatcat atcgtgccac agtcattctt
     4921 gaaggatgat tctatcgata acaaggtgct caccaggtct gataagaaca ggggtaagag
     4981 tgataacgtg ccaagtgaag aggttgtgaa gaaaatgaag aactattgga ggcagctcct
     5041 caacgctaag ctcatcactc agagaaagtt cgataacttg actaaggctg agaggggagg
     5101 actctctgaa ttggataagg caggattcat caagaggcag cttgtggaaa ccaggcagat
     5161 cactaagcac gttgcacaga tcctcgattc taggatgaac accaagtacg atgagaacga
     5221 taagttgatc agggaagtga aggttatcac cctcaagtca aagctcgtgt ctgatttcag
     5281 aaaggatttc caattctaca aggtgaggga aatcaacaac taccaccacg ctcacgatgc
     5341 ttaccttaac gctgttgttg gaaccgctct catcaagaag tatcctaagc tcgagtcaga
     5401 gttcgtgtac ggtgattaca aggtgtacga tgtgaggaag atgatcgcta agtctgagca
     5461 agagatcgga aaggctaccg ctaagtattt cttctactct aacatcatga atttcttcaa
     5521 gaccgagatt accctcgcta acggtgagat cagaaagagg ccactcatcg agacaaacgg
     5581 tgaaacaggt gagatcgtgt gggataaggg aagggatttc gctaccgtta gaaaggtgct
     5641 ctctatgcca caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg gattctctaa
     5701 agagtctatc ctccctaaga ggaactctga taagctcatt gctaggaaga aggattggga
     5761 ccctaagaaa tacggtggtt tcgattctcc taccgtggct tactctgttc tcgttgtggc
     5821 taaggttgag aagggaaaga gtaagaagct caagtctgtt aaggaacttc tcggaatcac
     5881 tatcatggaa aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg ctaagggata
     5941 caaagaggtt aagaaggatc tcatcatcaa gctcccaaag tactcactct tcgaactcga
     6001 gaacggtaga aagaggatgc tcgcttctgc tggtgagctt caaaagggaa acgagcttgc
     6061 tctcccatct aagtacgtta actttcttta cctcgcttct cactacgaga agttgaaggg
     6121 atctccagaa gataacgagc agaagcaact tttcgttgag cagcacaagc actacttgga
     6181 tgagatcatc gagcagatct ctgagttctc taaaagggtg atcctcgctg atgcaaacct
     6241 cgataaggtg ttgtctgctt acaacaagca cagagataag cctatcaggg aacaggcaga
     6301 gaacatcatc catctcttca cccttaccaa cctcggtgct cctgctgctt tcaagtactt
     6361 cgatacaacc atcgatagga agagatacac ctctaccaaa gaagtgctcg atgctaccct
     6421 catccatcag tctatcactg gactctacga gactaggatc gatctctcac agctcggtgg
     6481 tgattcaagg gctgatccta agaagaagag gaaggtttga ctcgagatat gaagatgaag
     6541 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg
     6601 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta
     6661 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca
     6721 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataaggagc
     6781 tcaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     6841 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
     6901 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
     6961 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
     7021 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
     7081 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
     7141 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
     7201 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
     7261 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
     7321 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
     7381 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
     7441 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
     7501 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
     7561 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
     7621 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
     7681 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
     7741 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
     7801 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
     7861 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
     7921 ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
     7981 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
     8041 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
     8101 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
     8161 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
     8221 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
     8281 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
     8341 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
     8401 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
     8461 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
     8521 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
     8581 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
     8641 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
     8701 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
     8761 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
     8821 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
     8881 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
     8941 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
     9001 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
     9061 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
     9121 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
     9181 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
     9241 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
     9301 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
     9361 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
     9421 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
     9481 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
     9541 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
     9601 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
     9661 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
     9721 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
     9781 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
     9841 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
     9901 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
     9961 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
    10021 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
    10081 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
    10141 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
    10201 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
    10261 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg
    10321 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
    10381 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
    10441 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
    10501 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
    10561 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
    10621 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
    10681 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
    10741 tgaaaacctc tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc
    10801 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    10861 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    10921 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10981 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    11041 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    11101 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    11161 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    11221 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    11281 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    11341 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    11401 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    11461 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    11521 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    11581 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    11641 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    11701 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11761 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11821 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
    11881 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    11941 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    12001 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    12061 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    12121 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    12181 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    12241 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    12301 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    12361 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    12421 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    12481 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    12541 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    12601 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    12661 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    12721 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    12781 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    12841 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    12901 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    12961 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    13021 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    13081 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    13141 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
    13201 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
    13261 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
    13321 tgggaactac tcacacatta ttatggagaa aatagagctt gtcgatcgtt aattaatcac
    13381 ctccaagatc ccggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
    13441 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
    13501 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
    13561 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
    13621 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
    13681 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
    13741 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
    13801 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
    13861 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
    13921 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
    13981 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
    14041 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
    14101 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
    14161 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
    14221 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
    14281 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggacacg
    14341 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
    14401 atcctaggag agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat
    14461 gaaatgaact tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc
    14521 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
    14581 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
    14641 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
    14701 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
    14761 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
    14821 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac
    14881 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    14941 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    15001 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    15061 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    15121 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    15181 ctccaccatg
//
