LOCUS       sequence-266264-       15997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iSTU-CRISPR/Cas9 with OsCDPK2 intron, tRNA structure backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Zhong Z, Liu S, Liu X, Liu B, Tang X, Ren Q, Zhou J, Zheng X, Qi Y, 
            Zhang Y
  TITLE     Intron-Based Single Transcript Unit CRISPR Systems for Plant Genome 
            Editing.
  JOURNAL   Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.1186/s12284-020-0369-8.
  PUBMED    32016614
REFERENCE   2  (bases 1 to 15997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Rice (N 
            Y)."; date: "2020-02-3"; volume: "13(1):8. doi"; pages: " 
            10.1186/s12284-020-0369-8"
COMMENT     Sequence Label: pGEL081
FEATURES             Location/Qualifiers
     source          1..15997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2004
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             complement(3574..3879)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(3676..3695)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        4206..4281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4907..7291
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7304..7324
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7335..7583
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7604..7628
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8928..9557
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9991..11058
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11124..11318
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11554..11576)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11662..11802
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11717..11736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11817..11834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11988..12576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12068..12087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12663..13457)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13882..13906
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13984..14158
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(15248..15925)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 ggcgcgcctg cagtgcagcg tgacccggtc gtgcccctct cttgagataa tgagcattgc
       61 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
      121 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
      181 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
      241 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
      301 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
      361 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
      421 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
      481 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
      541 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
      601 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
      661 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
      721 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
      781 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gaactccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttcaag atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt
     1441 ttggatctgt atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga
     1501 aatatcgatc taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga
     1561 tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca
     1621 agatcggagt agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat
     1681 gtgtgtgtca tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga
     1741 taggtataca tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc
     1801 tattcatatg ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat
     1861 tattttgatc ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt
     1921 agccctgcct tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc
     1981 tgttgtttgg tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag
     2041 tactctatcg gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag
     2101 tacaaggtgc catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag
     2161 aaaaacctta tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc
     2221 aagagaaccg ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag
     2281 atcttctcta acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca
     2341 ttcctcgtgg aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat
     2401 gaggtggcat accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat
     2461 tctactgata aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc
     2521 agaggacact tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg
     2581 ttcatccagc tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca
     2641 ggtgtggatg ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac
     2701 ctcattgctc agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc
     2761 tctctcggac tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc
     2821 cagctctcaa aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat
     2881 cagtacgctg atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat
     2941 atcctcagag tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga
     3001 tacgatgagc accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca
     3061 gagaagtaca aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat
     3121 ggtggtgcat ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat
     3181 ggaaccgagg aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc
     3241 ttcgataacg gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga
     3301 aggcaagagg atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc
     3361 accttcagaa tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg
     3421 atgaccagaa agtctgagga aaccatcacc ccttggaact tcgaagaggt caatatgctg
     3481 tcaaaggcaa acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga
     3541 cccgggttcg attcccggct ggtgcaggag accttatatt ccccagaaca tcaggttaat
     3601 ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga
     3661 aaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac
     3721 caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat
     3781 caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata
     3841 acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt
     3901 cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt
     3961 ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca
     4021 gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt
     4081 cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca
     4141 gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta tctggcttgg
     4201 tctcagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     4261 aaagtggcac cgagtcggtg caacaaagca ccagtggtct agtggtagaa tagtaccctg
     4321 ccacggtaca gacccgggtt cgattcccgg ctggtgcatt tcttttcttt tccttttcgt
     4381 tgcttgtttt ccccttctga tgttgtgtgt tgagtttatt gtttggtttg ctggtagatc
     4441 cagtatttgg atcatttttc cttttttttt tctttcattg cttctgcgga gggatgagtt
     4501 tgtgcatgaa tcttttgttg gttggattcg cttagttttc acactagtcc tttgattcag
     4561 cgcaaatctt tgttaattgg ctcgcttaat tctgacataa agtgttcctt cttggttctg
     4621 cttgcaggtg gtggataagg gtgctagtgc tcagtctttc atcgagagga tgaccaactt
     4681 cgataagaac cttccaaacg agaaggtgct ccctaagcac tctttgctct acgagtactt
     4741 caccgtgtac aacgagttga ccaaggttaa gtacgtgacc gagggaatga ggaagcctgc
     4801 ttttttgtca ggtgagcaaa agaaggctat cgttgatctc ttgttcaaga ccaacagaaa
     4861 ggtgaccgtg aagcagctca aagaggatta cttcaagaaa atcgagtgct tcgattcagt
     4921 tgagatttct ggtgttgagg ataggttcaa cgcatctctc ggaacctacc acgatctcct
     4981 caagatcatt aaggataagg atttcttgga taacgaggaa aacgaggata tcttggagga
     5041 tatcgttctt accctcaccc tctttgaaga tagagagatg attgaagaaa ggctcaagac
     5101 ctacgctcat ctcttcgatg ataaggtgat gaagcagttg aagagaagaa gatacactgg
     5161 ttggggaagg ctctcaagaa agctcattaa cggaatcagg gataagcagt ctggaaagac
     5221 aatccttgat ttcctcaagt ctgatggatt cgctaacaga aacttcatgc agctcatcca
     5281 cgatgattct ctcaccttta aagaggatat ccagaaggct caggtttcag gacagggtga
     5341 tagtctccat gagcatatcg ctaacctcgc tggatctcct gcaatcaaga agggaatcct
     5401 ccagactgtg aaggttgtgg atgagttggt gaaggtgatg ggaaggcata agcctgagaa
     5461 catcgtgatc gaaatggcta gagagaacca gaccactcag aagggacaga agaactctag
     5521 ggaaaggatg aagaggatcg aggaaggtat caaagagctt ggatctcaga tcctcaaaga
     5581 gcaccctgtt gagaacactc agctccagaa tgagaagctc tacctctact acctccagaa
     5641 cggaagggat atgtatgtgg atcaagagtt ggatatcaac aggctctctg attacgatgt
     5701 tgatcatatc gtgccacagt cattcttgaa ggatgattct atcgataaca aggtgctcac
     5761 caggtctgat aagaacaggg gtaagagtga taacgtgcca agtgaagagg ttgtgaagaa
     5821 aatgaagaac tattggaggc agctcctcaa cgctaagctc atcactcaga gaaagttcga
     5881 taacttgact aaggctgaga ggggaggact ctctgaattg gataaggcag gattcatcaa
     5941 gaggcagctt gtggaaacca ggcagatcac taagcacgtt gcacagatcc tcgattctag
     6001 gatgaacacc aagtacgatg agaacgataa gttgatcagg gaagtgaagg ttatcaccct
     6061 caagtcaaag ctcgtgtctg atttcagaaa ggatttccaa ttctacaagg tgagggaaat
     6121 caacaactac caccacgctc acgatgctta ccttaacgct gttgttggaa ccgctctcat
     6181 caagaagtat cctaagctcg agtcagagtt cgtgtacggt gattacaagg tgtacgatgt
     6241 gaggaagatg atcgctaagt ctgagcaaga gatcggaaag gctaccgcta agtatttctt
     6301 ctactctaac atcatgaatt tcttcaagac cgagattacc ctcgctaacg gtgagatcag
     6361 aaagaggcca ctcatcgaga caaacggtga aacaggtgag atcgtgtggg ataagggaag
     6421 ggatttcgct accgttagaa aggtgctctc tatgccacag gtgaacatcg ttaagaaaac
     6481 cgaggtgcag accggtggat tctctaaaga gtctatcctc cctaagagga actctgataa
     6541 gctcattgct aggaagaagg attgggaccc taagaaatac ggtggtttcg attctcctac
     6601 cgtggcttac tctgttctcg ttgtggctaa ggttgagaag ggaaagagta agaagctcaa
     6661 gtctgttaag gaacttctcg gaatcactat catggaaagg tcatctttcg agaagaaccc
     6721 aatcgatttc ctcgaggcta agggatacaa agaggttaag aaggatctca tcatcaagct
     6781 cccaaagtac tcactcttcg aactcgagaa cggtagaaag aggatgctcg cttctgctgg
     6841 tgagcttcaa aagggaaacg agcttgctct cccatctaag tacgttaact ttctttacct
     6901 cgcttctcac tacgagaagt tgaagggatc tccagaagat aacgagcaga agcaactttt
     6961 cgttgagcag cacaagcact acttggatga gatcatcgag cagatctctg agttctctaa
     7021 aagggtgatc ctcgctgatg caaacctcga taaggtgttg tctgcttaca acaagcacag
     7081 agataagcct atcagggaac aggcagagaa catcatccat ctcttcaccc ttaccaacct
     7141 cggtgctcct gctgctttca agtacttcga tacaaccatc gataggaaga gatacacctc
     7201 taccaaagaa gtgctcgatg ctaccctcat ccatcagtct atcactggac tctacgagac
     7261 taggatcgat ctctcacagc tcggtggtga ttcaagggct gatcctaaga agaagaggaa
     7321 ggtttgactc gagatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg
     7381 tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta
     7441 atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta taatccattg
     7501 tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac
     7561 tatggaatat gattaaagat aaggagctca aactatcagt gtttgacagg atatattggc
     7621 gggtaaacct aagagaaaag agcgtttatt agaataacgg atatttaaaa gggcgtgaaa
     7681 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     7741 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     7801 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     7861 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     7921 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     7981 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     8041 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     8101 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     8161 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     8221 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     8281 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
     8341 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
     8401 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
     8461 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
     8521 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
     8581 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
     8641 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
     8701 gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc
     8761 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
     8821 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     8881 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     8941 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     9001 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     9061 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     9121 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     9181 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     9241 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     9301 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     9361 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     9421 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     9481 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     9541 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     9601 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     9661 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     9721 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     9781 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     9841 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     9901 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     9961 gaacccccaa gcccgaggaa tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc
    10021 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
    10081 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
    10141 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
    10201 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
    10261 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
    10321 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
    10381 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
    10441 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
    10501 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
    10561 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
    10621 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
    10681 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
    10741 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
    10801 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
    10861 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
    10921 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
    10981 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
    11041 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
    11101 aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
    11161 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
    11221 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
    11281 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
    11341 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
    11401 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
    11461 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
    11521 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac
    11581 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    11641 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    11701 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    11761 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    11821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    11941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    12301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    12361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    12421 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    12481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    12541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    12601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcattctagg
    12661 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
    12721 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
    12781 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
    12841 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
    12901 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
    12961 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
    13021 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
    13081 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
    13141 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
    13201 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
    13261 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
    13321 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
    13381 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
    13441 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
    13501 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacctta
    13561 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
    13621 cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat cgttacaatc
    13681 aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag ttgccgttct
    13741 tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc tcccgctgac
    13801 gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc
    13861 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
    13921 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg
    13981 ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa
    14041 gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa
    14101 ccctatagga accctaattc ccttatctgg gaactactca cacattatta tggagaaaat
    14161 agagcttgtc gatcgttaat taatcacctc caagatcccg gacgagtgct ggggcgtcgg
    14221 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
    14281 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
    14341 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
    14401 ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc
    14461 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
    14521 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
    14581 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
    14641 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
    14701 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
    14761 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
    14821 gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca
    14881 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg
    14941 caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg
    15001 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
    15061 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
    15121 tccgagagct gcatcaggtc ggacacgctg tcgaactttt cgatcagaaa cttctcgaca
    15181 gacgtcgcgg tgagttcagg ctttttcatc ctaggagaga tagatttgta gagagagact
    15241 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg
    15301 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
    15361 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    15421 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    15481 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    15541 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    15601 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    15661 ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    15721 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    15781 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    15841 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    15901 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    15961 attcttggag tagacgagag tgtcgtgctc caccatg
//
