LOCUS       sequence-266307-        6649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive mammalian co-expression vector for human bone 
            morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, 
            divergent).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Feichtinger GA, Hacobian A, Hofmann AT, Wassermann K, Zimmermann A, 
            van Griensven M, Redl H
  TITLE     Constitutive and inducible co-expression systems for non-viral 
            osteoinductive gene therapy.
  JOURNAL   Eur Cell Mater. 2014 Feb 19;27:166-84; discussion 184.
  PUBMED    24554273
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur Cell 
            Mater."; date: "2014-02-19"; volume: "27"; pages: "166-84; 
            discussion 184"
COMMENT     Sequence Label: pVAX1-BMP2/7 -
FEATURES             Location/Qualifiers
     source          1..6649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      764..773
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      768..777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2042..2266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2439..3233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2439..3233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2493..2512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3103..3122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3424..3648
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(5195..5213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5258..5461)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        5462..5841
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(5942..6530)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6022..6041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggtcatgtc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      721 accgagctcg gatccactag tccagtgtgg tggaattcgg cttgccacca tggtggccgg
      781 gacccgctgt cttctagcgt tgctgcttcc ccaggtcctc ctgggcggcg cggctggcct
      841 cgttccggag ctgggccgca ggaagttcgc ggcggcgtcg tcgggccgcc cctcatccca
      901 gccctctgac gaggtcctga gcgagttcga gctgcggctg ctcagcatgt tcggcctgaa
      961 acagagaccc acccccagca gggacgccgt ggtgcccccc tacatgctag acctgtatcg
     1021 caggcactca ggtcagccgg gctcacccgc cccagaccac cggttggaga gggcagccag
     1081 ccgagccaac actgtgcgca gcttccacca tgaagaatct ttggaagaac taccagaaac
     1141 gagtgggaaa acaacccgga gattcttctt taatttaagt tctatcccca cggaggagtt
     1201 tatcacctca gcagagcttc aggttttccg agaacagatg caagatgctt taggaaacaa
     1261 tagcagtttc catcaccgaa ttaatattta tgaaatcata aaacctgcaa cagccaactc
     1321 gaaattcccc gtgaccagac ttttggacac caggttggtg aatcagaatg caagcaggtg
     1381 ggaaagtctt gatgtcaccc ccgctgtgat gcggtggact gcacagggac acgccaacca
     1441 tggattcgtg gtggaagtgg cccacttgga ggagaaacaa ggtgtctcca agagacatgt
     1501 taggataagc aggtctttgc accaagatga acacagctgg tcacagataa ggccattgct
     1561 agtaactttt ggccatgatg gaaaagggca tcctctccac aaaagagaaa aacgtcaagc
     1621 caaacacaaa cagcggaaac gccttaagtc cagctgtaag agacaccctt tgtacgtgga
     1681 cttcagtgac gtggggtgga atgactggat tgtggctccc ccggggtatc acgcctttta
     1741 ctgccacgga gaatgccctt ttcctctggc tgatcatctg aactccacta atcatgccat
     1801 tgttcagacg ttggtcaact ctgttaactc taagattcct aaggcatgct gtgtcccgac
     1861 agaactcagt gctatctcga tgctgtacct tgacgagaat gaaaaggttg tattaaagaa
     1921 ctatcaggac atggttgtgg agggttgtgg gtgtcgctag aagccgaatt ctgcagatat
     1981 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     2041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tactgggcgg
     2281 ttttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt aaggttggga
     2341 agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg cgcaggggat
     2401 caagctctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2941 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaattatta
     3241 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3301 gcatcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3361 atacattcaa atatgtatcc gctcatgtgt tcgcttgctg tccataaaac cgcccagtag
     3421 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
     3481 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
     3541 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
     3601 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
     3661 agcgggttta aacgggccct ctagactcga gcggccgctg ctgctcatgt ttcctaatac
     3721 tctcacacct ttaaagttgg ggatagggag gggaaggtct cacaaaaggc agttggtctg
     3781 ctggttcctg gccaaggcga gcaatggagg atccagaaaa acttggcccc aaagggtctg
     3841 aattctcgga ggagctagtg gcagccacag gcccggacca ccatgtttct gtatttcttc
     3901 aggatgacgt tggagctgtc atcgaagtag aggacggaga tggcattgag ctgcgtgggc
     3961 gcacagcagg gcttgggcac cgtttccggg ttgatgaagt ggaccagcgt ctgcacgatg
     4021 gcgtggttgg tggcgttcat gtaggagttc agagggaagg cacactcccc ctcacagtag
     4081 taggcggcgt agccttcagg cgcgatgatc cagtcctgcc agcccaggtc tcggaagctg
     4141 acatacagct cgtgcttctt acaggcctgc ctctggtcgc tgctgctgtt ctctgccacg
     4201 ttggccatcc gcagggcttc ctggttcttg ggcgtcttgg agcggttctg gctgcgctgt
     4261 ttgctccccg tggaccggat gctgcggaag tggacctccg tggccttgaa gaaagccacc
     4321 atgaagggct gcttgttctg gggcccgtgc cgcccaatca ggcccgccaa cttggggttg
     4381 atgctctgcc catccagcgt ctccaccgag agctgcaggc ccaggttgtg ccgcggattg
     4441 accacccagt ggttgctggt ggctgtgatg tcaaacacca gccagccctc ctccgaggcc
     4501 cagagggtac ggctgtcgag caggaagaga tccgattccc tgcccaagtg ctcctggagc
     4561 acctgataaa cgctgatccg gaacgtctca ttgtcgaagc gttcccggat gtagtccttg
     4621 tagatccgga attcggctgc cgtgacagct tccccttctg ggatcttgga aagatcaaac
     4681 cggaactctc gatggtggta gcgtgggtgg aagaattcct tgtcatgttc cacgaggttg
     4741 acgaagctca tgaccatgtc ggcgtcggtg aggaaatggc tatcttgcag gctggccaga
     4801 ggggggccct gggtactgaa gacggccttg taggggtagg agaagccctg gccgccgggc
     4861 ccgccgccct cctccaccgc catggcgttg tacaggtcca gcatgaacat gggtgccgag
     4921 ttgtgcttgc cctggaggtg cgggcgcggg cggtggggca agcccaaaat ggagaggatc
     4981 tcgcgctgca tctcccgccg ctcctggctg cggaggcgcc ggtggatgaa gctcgagtgc
     5041 acctcgttgt ccaggctgaa gtcggccagg gcggagcgca gcaggaacag gggtgcccag
     5101 agcgccacga agctgtgcgg cgccgcagct cgcagtgagc gcacgtgcat cgcggtggca
     5161 agcttaagtt taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga
     5221 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     5281 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     5341 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     5401 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     5461 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5521 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5581 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5641 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5701 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5761 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5821 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcaaga
     5881 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5941 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6001 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6061 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6121 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6181 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6241 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6301 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6361 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6421 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6481 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6541 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6601 tcatgtcgat gtacgggcca gatatacgcg ttgacattga ttattgact
//
