LOCUS       sequence-266455-        3232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IPTG inducible expression of Q-TAGA qtRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   DeBenedictis EA, Carver GD, Chung CZ, Soll D, Badran AH
  TITLE     Multiplex suppression of four quadruplet codons via tRNA directed 
            evolution.
  JOURNAL   Nat Commun. 2021 Sep 29;12(1):5706. doi: 10.1038/s41467-021-25948-y.
  PUBMED    34588441
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-09-29"; volume: "12(1):5706. doi"; pages: " 
            10.1038/s41467-021-25948-y"
COMMENT     Sequence Label: pED14xQ-1
FEATURES             Location/Qualifiers
     source          1..3232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        534..568
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     CDS             595..1743
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     promoter        1753..1844
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1845..2636
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     protein_bind    2923..2939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
       61 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
      121 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
      181 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
      241 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
      301 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
      361 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
      421 cccccttctc gaaccctccc ggcccgctaa cgcgggcctc ccatcccccc aggggctgcg
      481 cccctcggcc gcgaacggcc tcaccccaaa aatggaggtt tctcttcttt cactttacgg
      541 ctagctcagc cctaggtatt atgctagcag ccccatacga tataaggagg gtgaatgaat
      601 cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag
      661 gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc
      721 gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct
      781 ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag
      841 gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag
      901 cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg
      961 ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg
     1021 tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg
     1081 cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag
     1141 gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg
     1201 cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc
     1261 tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag
     1321 gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac
     1381 ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc
     1441 cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg
     1501 aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat
     1561 ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga
     1621 tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg
     1681 cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc
     1741 tagacggcct atttggccta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1801 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagg gaagcggtga
     1861 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     1921 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     1981 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2041 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2101 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2161 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2221 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2281 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2341 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2401 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2461 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     2521 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     2581 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaacgc
     2641 catgggcatt aaaaaatgcc ctcttgggtt atcaagaggg tcattatatt tgctgaggag
     2701 tgccgttaat taagtcggat gaatggcaga agcttaaaaa aaatccttag ctttcgctaa
     2761 ggatctgcag gtcgacggat cctcgaagcg agctttttta atgtggctgg ggtacgagga
     2821 ttcgaacctc ggaatgccgg aattagaaat ccggtgcctt accgcttggc gataccccaa
     2881 ctgggtgcac ttacaaggta agggacccat cttgtctgct aattgttatc cgctcacaat
     2941 tatcttactg cgcagatacg ccctcgtcaa tcccttaata gcaaaatgcc ttttgatcgg
     3001 cgagaaagtc agcaggccgc ttagttagcc gtgcactgat catatcgtca attattacct
     3061 ccacggggag agcctgagca aactggcctc aggcatttga gaagcacaag ggtccctaag
     3121 tctcctcagc aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgaccgg
     3181 atgtcctctt actgacagat gaggggcgga cgttgacact tgaggggccg ac
//
