LOCUS       sequence-266501-        1362 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   O'Day E, Le MT, Imai S, Tan SM, Kirchner R, Arthanari H, Hofmann O, 
            Wagner G, Lieberman J
  TITLE     An RNA-binding Protein, Lin28, Recognizes and Remodels G-quartets in
            the MicroRNAs (miRNAs) and mRNAs It Regulates.
  JOURNAL   J Biol Chem. 2015 Jul 17;290(29):17909-22. doi: 
            10.1074/jbc.M115.665521. Epub 2015 Jun 4.
  PUBMED    26045559
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.665521"; journalName: "J Biol Chem"; date: 
            "2015-07-17- 17"; volume: "290"; issue: "29"; pages: "17909-22"
COMMENT     Sequence Label: pET15b-mLin28A-cHIS
FEATURES             Location/Qualifiers
     source          1..1362
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..63)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             532..554
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    569..593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(593..612)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(594..612)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(696..715)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 acaatagctt cctttgggct tgttagcagc cggatcctcg agttagtggt gatggtgatg
       61 atgccctccc tgtgcactag ggccctgctg ggccttcagc ggacatgagg ctaccatatg
      121 gctgatgctc tggcagaagt ggcacttctt gggctggggt ggcagcttgc attccttggc
      181 atgatgatct agacctccac agttgtagca cctgtctcct tttgatctgc gcttctgcat
      241 gctctttcct tttggccgcc tctcactccc aatacagaat actccaccag gtccggtgac
      301 acggatggat tccagaccct tggctgactt cttaaaggtg aactccactg cctcaccctc
      361 cttcaagctc cggaaccctt ccatgtgcag cttactctgg tgcacaaaga catccactgg
      421 ggggtcgagc gcgaccccgg cgcgggcggt catggacagg aagccgaacc ccatgcgcac
      481 gttgaaccac ttacagatgc ccgcaccgtg cagcagctgc cccatggtat atctccttct
      541 taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
      601 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
      661 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      721 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      781 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      841 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
      901 gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
      961 gatagcgccc ggaaaanant caatncaggg tggtgaatgt gaaaccagta acgttatacn
     1021 atgtcccaaa atatgccggt gtctcttatc aaanngtttn ccccggnggg naacaaggcc
     1081 ancccngttt ntgcnaaaac cnggaaaaaa nggaaacngc nangggngaa attnaattan
     1141 nttcccancn ggggggnaaa aaatgggggg aaaaaaannt tnnngantgg ggtnncnccc
     1201 cnantnggnc cnnnaanncc nnnnaaaatt nnnnggnaaa aantcccccc naaaantggg
     1261 nncnncnggg gggnnnannn naaaaaaaan gnnnnaaacn nnaaangggg ggnaannttn
     1321 ccnnnnnnnn ggggggnant tanannncng gnaaaaaaaa tt
//
