LOCUS       sequence-266619-        6928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM
  TITLE     Single-cell analysis of Daxx and ATRX-dependent transcriptional 
            repression.
  JOURNAL   J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 
            10.1242/jcs.110148. Epub 2012 Sep 12.
  PUBMED    22976303
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-11-15"; volume: "125(Pt 22):5489-501. doi"; 
            pages: " 10.1242/jcs.110148. Epub 2012 Sep 12"
COMMENT     Sequence Label: YFP-ICP0FxE-C3
FEATURES             Location/Qualifiers
     source          1..6928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3656..3777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3693..3712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3747..3766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3784..4239)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3921..3942)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4133..4152
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4266..4370
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4372..4729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4372..4563
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4395..4415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4580..4715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4764..5558
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4764..5558
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4818..4837)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5428..5447
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5746..5765)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5790..5837
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6166..6754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6655..6674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
     1261 ctgtacaagt actcagatct cgacatggag ccccgccccg gagcgagtac ccgccggcct
     1321 gagggccgcc cccagcgcga gcccgccccg gatgtctggg tgtttccctg cgaccgagac
     1381 ctgccggaca gcagcgactc tgaggcggag accgaagtgg gggggcgggg ggacgccgac
     1441 caccatgacg acgactccgc ctccgaggcg gacagcacgg acacggaact gttcgagacg
     1501 gggctgctgg ggccgcaggg cgtggatggg ggggcggtct cgggggggag ccccccccgc
     1561 gaggaagacc ccggcagttg cgggggcgcc ccccctcgag gaattccaat tcggggcaac
     1621 acctgcccgc tgtgcaacgc caagctggtg tacctgatag tgggcgtgac gcccagcggg
     1681 tcgttcagca ccatcccgat cgtgaacgac ccccagaccc gcatggaggc cgaggaggcc
     1741 gtcagggcgg gcacggccgt ggactttatc tggacgggca atcagcggtt cgccccgcgg
     1801 tacctgaccc tgggggggca cacggtgagg gccctgtcgc ccacccaccc ggagcccacc
     1861 acggacgagg atgacgacga cctggacgac gcagactacg taccgcccgc cccccgccgg
     1921 acgccccgcg cccccccacg cagaggcgcc gccgcgcccc ccgtgacggg cggggcgtct
     1981 cacgcagccc cccagccggc cgcggctcgg acagcgcccc cctcggcgcc catcgggcca
     2041 cacggcagca gtaacaccaa caccaccacc aacagcagcg gcggcggcgg ctcccgccag
     2101 tcgcgagccg cggcgccgcg gggggcgtct ggcccctccg ggggggttgg ggttggggtt
     2161 ggggttgttg aagcggaggc ggggcggccg aggggccgga cgggccccct tgtcaacaga
     2221 cccgcccccc ttgcaaacaa cagagacccc atagtgatca gcgactcccc cccggcctct
     2281 ccccacaggc cccccgcggc gcccatgcca ggctccgccc cccgccccgg gccccccgcg
     2341 tccgcggccg cgtcgggacc cgcgcgcccc cgcgcggccg tggccccgtg cgtgcgagcg
     2401 ccgcctccgg ggcccggccc ccgcgccccg gcccccgggg cggagccggc cgcccgcccc
     2461 gcggacgcgc gccgtgtgcc ccagtcgcac tcgtccctgg ctcaggccgc gaaccaagaa
     2521 cagagtctgt gccgggcgcg tgcgacggtg gcgcgcggct cgggggggcc gggcgtggag
     2581 ggtgggcacg ggccctcccg cggcgccgcc ccctccggcg ccgccccgct cccctccgcc
     2641 gcctctgtcg agcaggaggc ggcggtgcgt ccgaggaaga ggcgcgggtc gggccaggaa
     2701 aacccctccc cccagtccac gcgtcccccc ctcgcgccgg caggggccaa gagggcggcg
     2761 acgcaccccc cctccgactc agggccgggg gggcgcggcc agggtgggcc cgggaccccc
     2821 ctgacgtcct cggcggcctc cgcctcttcc tcctctgcct cttcctcctc ggccccgacc
     2881 cccgcggggg ccgcctcttc cgccgccggg gccgcgtcct cctccgcttc cgcctcctcg
     2941 ggcggggccg tcggtgccct gggagggaga caagaggaaa cctccctcgg cccccgcgct
     3001 gcttctgggc cgcgggggcc gaggaagtgt gcccggaaga cgcgccacgc ggagacttcc
     3061 ggggccgtcc ccgcgggcgg cctcacgcgc tacctgccca tctcgggggt ctctagcgtg
     3121 gtcgccctgt cgccttacgt gaacaagact atcacggggg actgcctgcc catcctggac
     3181 atggagacgg ggaacatcgg ggcgtacgcg gtcctggtgg accagacggg aaacatggcg
     3241 acccggctgc gggccgcggt ccccggctgg agccgccgca ccctgctccc cgagaccgcg
     3301 ggtaaccacg tgatgccccc cgagtacccg acggcccccg cgtcggagtg gaacagcctc
     3361 tggatgaccc ccgtggggaa catgctgttc gaccagggca ccctagtggg cgccctggac
     3421 ttccgcagcc tgcggtctcg gcacccgtgg tccggggagc agggggcgtc gacccgggac
     3481 gagggaaaac aataagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc
     3541 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3601 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     3661 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     3721 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     3781 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3841 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3901 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3961 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4021 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4081 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4141 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4201 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4261 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4321 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     4381 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     4441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     4501 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     4561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     4741 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4801 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4861 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4921 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     4981 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5041 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5101 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5161 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5221 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5281 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     5341 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     5401 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     5461 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     5521 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     5581 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5641 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5701 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     5761 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     5821 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     5881 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     5941 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     6001 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6841 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     6901 tagttcatag cccatatatg gagttccg
//
