LOCUS       sequence-26668-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Enslen H, Raingeaud J, Davis RJ
  TITLE     Selective activation of p38 mitogen-activated protein (MAP) kinase 
            isoforms by the MAP kinase kinases MKK3 and MKK6.
  JOURNAL   J Biol Chem. 1998 Jan 16. 273(3):1741-8.
  PUBMED    9430721
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Jan 16. 273(3):1741-8."
COMMENT     Sequence Label: pCDNA3 Flag p38 gamma
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatcccggc agccaacatg gactataagg
      121 acgatgacga caaaagctcc ccgccacccg cccgcaaggg cttttaccgc caggaggtga
      181 ccaaaacggc ctgggaggtg cgcgccgtgt accaggacct gcagcccgtt ggctctggtg
      241 cctatggtgc agtgtgctct gcagtagaca gccgcactgg caacaaggtg gccatcaaga
      301 agttgtaccg gcccttccag tcggagctgt ttgccaagcg cgcctacaga gagttgcgcc
      361 tcctcaaaca catgcgccac gagaacgtca ttgggctgct ggatgtgttc actcccgatg
      421 agactctgga tgacttcaca gacttctacc tggtgatgcc attcatgggc actgacctgg
      481 gcaagctcat gaagcacgag actctgagtg aagacagaat ccagtttctt gtgtatcaga
      541 tgctgaaggg gctgaagtat atccacgctg ccggcgtcat ccacagggac ttgaaacctg
      601 gaaacctggc tgtgaacgag gactgtgagc tgaagatcct agattttggc cttgccaggc
      661 aggcggacag tgagatgaca ggatatgtgg taacccggtg gtatcgggca ccagaggtca
      721 tcttgaattg gat
//
