LOCUS       sequence-266734-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR SONIC:  HRAS G12V donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Mou H, Ozata DM, Smith JL, Sheel A, Kwan SY, Hough S, Kucukural A, 
            Kennedy Z, Cao Y, Xue W
  TITLE     CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in 
            vivo cancer modeling.
  JOURNAL   Genome Med. 2019 Apr 16;11(1):21. doi: 10.1186/s13073-019-0627-9.
  PUBMED    30987660
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Med."; date: "2019-04-16"; volume: "11(1):21. doi"; pages: " 
            10.1186/s13073-019-0627-9"
COMMENT     Sequence Label: H517 SONIC HRASV12 donor
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..33,34..36,37..570)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 atgacggaat ataagctggt ggtggtgggc gccgtcggtg tgggcaagag tgcgctgacc
       61 atccagctga tccagaacca ttttgtggac gaatacgacc ccactataga ggattcctac
      121 cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgccggc
      181 caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt
      241 gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc
      301 aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg
      361 gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc
      421 tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta cacgttggtg
      481 cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc
      541 tgcatgagct gcaagtgtgt gctctcctga
//
