LOCUS       sequence-26691-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Dupont S, Zacchigna L, Cordenonsi M, Soligo S, Adorno M, Rugge M, 
            Piccolo S
  TITLE     Germ-layer specification and control of cell growth by Ectodermin, a
            Smad4 ubiquitin ligase.
  JOURNAL   Cell. 2005 Apr 8. 121(1):87-99.
  PUBMED    15820681
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2005 
            Apr 8. 121(1):87-99."
COMMENT     Sequence Label: pCS2-FLAG-hEcto/Tif1g-CAmut
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     33..50
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct
       61 acttgttctt tttgcaggat cccatcgatt cgaattcaag gcgtttagtg aaccgtcaga
      121 attgatctac catggactac aaagacgatg acgacaagct tgcggccgcg aattcatcga
      181 tagatccgcc tctcaccgcg gtgctggtgg aggaggagga ggaggaaggc ggcagggccg
      241 gcgctgaggg cggcgcggcc gggcccgacg acgggggggt ggccgcggcc tcctcgggct
      301 cggcccaggc tgcttcatct cctgcggcct cagtgggcac tggagttgcc gggggcgcag
      361 tatcgacgcc ggctccagct ccagcctcgg ctcccgctcc gggtccctcg gcagggccgc
      421 ctcctggacc gccagcctcg ctcctggaca cggccgcagt ggcccagcag agcttgcaga
      481 gccggcgtga ggcggagccc aagctgctgc cctgtcttca ctccttctgc ctgcgctgcc
      541 tgcccgagcc ggagcgccag ctcagcgtgc ccatcccggg gggcagcaac ggcgacatcc
      601 agcaagttgg tgtaatacgg tgcccagtat gccgccnaga atgcagacag atagaccttg
      661 tgganaatta ttttgtgaaa gacacatctg aagctcctag cagttctgat gaaaaatc
//
