LOCUS       sequence-266921-       10802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10802)
  AUTHORS   Tan MJ, White EA, Sowa ME, Harper JW, Aster JC, Howley PM
  TITLE     Cutaneous beta-human papillomavirus E6 proteins bind Mastermind-like
            coactivators and repress Notch signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 30.
  PUBMED    22547818
REFERENCE   2  (bases 1 to 10802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Apr 30."
COMMENT     Sequence Label: p6873 pHAGE-N-V5-MAML1 (1-990)
FEATURES             Location/Qualifiers
     source          1..10802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2736..2754
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2785..2809
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2896..2905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2908..2949
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2965..2989
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5963..5987)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6006..6505
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6041..6064)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6438..6457
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6526..7125
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6526..6545)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7022..7042
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7192..7780
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7245..7265)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7663..7674)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7855..8088
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8207..8225)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8293..8397
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8398..8466,8467..9258)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8616..8635)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9429..10017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9918..9937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10171..10188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10428..10449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10464..10469,10470..10487,10488..10494)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10502..10518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10507..10529
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10526..10542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10526..10542
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10592..10611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata gagatctccc
     2761 tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg cctggagacg
     2821 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     2881 agcgtttaaa cgctagccac catggcgggt aagcctatcc ctaaccctct cctcggtctc
     2941 gattctacgg ctcgaggtta tcaaacaagt ttgtacaaaa aagttggcat ggtgctgccc
     3001 acctgcccca tggcggagtt cgcgctgccg cggcacagcg cggtcatgga gcgccttcgc
     3061 cggcgcatcg agctgtgccg gcgccaccac agcacctgcg aggcccgcta cgaggccgtg
     3121 tcgcccgagc gcctggagct ggagcgccaa cacaccttcg ccctgcacca gcgctgcatc
     3181 caggccaagg ccaagcgcgc cgggaagcac aggcagccgc ccgccgccac ggccccggcg
     3241 cccgccgccc cggccccgcg cctggacgcc gctgacggcc ccgagcacgg ccgcccggcc
     3301 acgcatcttc atgatacagt taagaggaat cttgacagcg ccacttcccc tcagaatggc
     3361 gatcaacaga atggctacgg ggacctcttt cctgggcata agaagactcg ccgggaggcc
     3421 cctctgggag ttgccatctc ttccaatgga ctgcctccag cctcccccct cggtcagtct
     3481 gacaagcctt ctggagccga cgccctgcag tccagtggga agcactctct ggggctagac
     3541 tctctcaaca aaaagcgtct ggctgactcc agccttcact tgaatggagg cagtaacccc
     3601 agtgagtcat ttcctctgag cctgaataaa gaactgaagc aggagcctgt cgaagacctg
     3661 ccttgcatga tcactgggac tgtcggctcc atatcgcaaa gcaacctcat gccagacctc
     3721 aaccttaacg agcaggagtg gaaggagctc atcgaggagc tgaacaggtc ggtgcccgat
     3781 gaagacatga aggacctgtt taatgaggac ttcgaggaga agaaggaccc agagtcttct
     3841 ggctctgcca cacaaacccc cttggcacag gacattaata ttaagacgga attctctcca
     3901 gcagcctttg agcaagaaca gttaggctct ccacaagtga gggccgggtc tgcagggcag
     3961 acctttctgg ggccttcctc tgcccctgtg agtacagatt cccccagcct agggggctcc
     4021 caaaccttat tccacacctc tggtcagccc cgggcggaca atcccagtcc aaacctgatg
     4081 ccggcatcag cccaggccca gaacgcacaa agagcccttg caggtgtggt attgcccagt
     4141 cagggcccag gaggggcctc agagctgtcc tctgcccacc agctccagca gatcgctgcc
     4201 aagcagaagc gcgagcagat gctccagaac ccacagcagg ccaccccggc accagccccg
     4261 ggccagatgt ccacatggca gcagacgggg ccctcccaca gttccttaga tgtcccttac
     4321 cccatggaga agcctgccag cccttccagc tacaagcaag acttcactaa ctccaaactg
     4381 ctcatgatgc ctagtgtgaa taagagttcc cctcggcccg gaggccccta cctccagccc
     4441 agccatgtga acctgctgag tcaccagcca ccgagtaact tgaatcagaa ctccgcgaat
     4501 aaccaggggt ctgtgctgga ctacggcaat acaaaacccc tttctcatta caaagcggac
     4561 tgtgggcaag gcagcccggg gtctggccag agcaagccag ccctgatggc ttatcttccc
     4621 cagcagctgt cccatataag tcacgagcag aactccctgt ttctgatgaa gccaaagcca
     4681 ggaaatatgc ctttccgatc actggttcca cctggccagg agcagaaccc ttccagtgtc
     4741 cctgtgcaag cccaggctac cagtgttggg acccagccgc ctgccgtgtc cgtggccagc
     4801 tcccacaaca gctcccccta tctcagcagc cagcaacagg ccgctgtaat gaagcagcat
     4861 cagttgcttt tggaccaaca gaaacaaagg gagcagcagc aaaagcattt acagcaacag
     4921 cagttccttc agaggcaaca gcaccttctc gcggaacagg agaagcaaca gtttcagcgc
     4981 catctgaccc gcccaccacc ccagtaccaa gacccgacac aaggcagctt cccacagcag
     5041 gttggacagt tcacagggtc ctctgctgcc gtgcccggca tgaacacctt gggtccatcc
     5101 aactccagct gtcctcgagt gttccctcag gctgggaatc tgatgccaat gggccctgga
     5161 catgcttcag tttcctctct ccccacaaac tcaggccaac aggaccgggg tgtggctcag
     5221 ttccctggct cccaaaacat gcctcagagc agcctctatg gcatggcttc tggcataacc
     5281 cagatagttg cccagccccc gccacaggcc accaatggac atgcccacat tccacggcag
     5341 accaacgtgg gccagaacac ctccgtctca gctgcctatg ggcagaactc tctgggaagc
     5401 tctggcctct cccagcagca caataagggg accctgaacc ctggtttaac aaagccaccg
     5461 gtcccaaggg tgtcaccagc catgggaggc cagaattcct cctggcagca tcagggaatg
     5521 ccgaacctca gtggccagac cccagggaac agcaacgtga gtcccttcac tgcagcctcc
     5581 agtttccaca tgcagcagca ggcccacctg aaaatgtcta gcccgcaatt ctcccaggca
     5641 gtgcccaaca ggcccatggc tcccatgagc tcagcagctg ccgtggggtc cttgctaccc
     5701 ccagtgagtg cacagcagag gaccagcgcc cctgccccag caccaccccc aacagcccct
     5761 cagcagggct tgcctggcct gagcccagca gggcctgagc tgggggcctt cagccagagc
     5821 cctgcctcac agatgggcgg tcgggcgggg ctgcactgca cccaggccta ccctgtgcgg
     5881 accgcgggcc aggagctgcc ttttgcctat agcgggcagc caggtggcag tgggctctct
     5941 agtgtggctg gacacacctg atacccaact ttcttgtaca aagtggttcg ataacgaatt
     6001 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc
     6061 gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg
     6121 taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct
     6181 agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt
     6241 agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg
     6301 cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg
     6361 aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag
     6421 gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc
     6481 ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag
     6541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6721 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6781 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6841 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6901 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6961 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     7021 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     7081 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
     7141 ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgatagatcc taatcaacct
     7201 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7261 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7321 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7381 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7441 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7501 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7561 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7621 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7681 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7741 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     7801 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7861 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7921 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7981 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8041 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     8101 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     8161 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     8221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     8281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8641 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     9001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     9061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     9121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     9181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     9241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     9301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     9361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     9421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     9481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     9541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     9601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     9661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     9721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     9781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     9841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    10021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    10081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    10141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    10201 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
    10261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    10321 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
    10381 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    10441 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    10501 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
    10561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    10621 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
    10681 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
    10741 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
    10801 gt
//
