LOCUS       sequence-266996-        1788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Mxi1, Part 3a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM726
FEATURES             Location/Qualifiers
     source          1..1788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..123
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(143..177)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(248..907)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     822..841
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1089..1677
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1578..1597
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atggaacgtg tgagaatgat taatgtgcaa aggctgttag aagccgcaga gtttttagaa
       61 agaagagaaa gagaatgcga acacgggtat gccagttctt tccctagcat gccctctccc
      121 agaggttctt gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
      181 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
      241 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
      301 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
      361 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
      421 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa
      481 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
      541 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
      601 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
      661 ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa
      721 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      781 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      841 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      901 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
      961 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc
     1021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1201 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1741 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcat
//
