LOCUS       sequence-267040-        5814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for dCas9 NLS, Part 3a(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM769
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4192..4212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(4247..4281)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4352..5011)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4926..4945
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5193..5781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5682..5701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
       61 aaccgtagtc ggtctcatat ggacaagaag tattctatcg gactggccat cgggactaat
      121 agcgtcgggt gggccgtgat cactgacgag tacaaggtgc cctctaagaa gttcaaggtg
      181 ctcgggaaca ccgaccggca ttccatcaag aaaaatctga tcggagctct cctctttgat
      241 tcaggggaaa ccgctgaagc aacccgcctc aagcggactg ctagacggcg gtacaccagg
      301 aggaagaacc ggatttgtta ccttcaagag atattctcca acgaaatggc aaaggtcgac
      361 gacagcttct tccataggct ggaagaatca ttcctcgtgg aagaggataa gaagcatgaa
      421 cggcatccca tcttcggtaa tatcgtcgac gaggtggcct atcacgagaa atacccaacc
      481 atctaccatc ttcgcaaaaa gctggtggac tcaaccgaca aggcagacct ccggcttatc
      541 tacctggccc tggcccacat gatcaagttc agaggccact tcctgatcga gggcgacctc
      601 aatcctgaca atagcgatgt ggataaactg ttcatccagc tggtgcagac ttacaaccag
      661 ctctttgaag agaaccccat caatgcaagc ggagtcgatg ccaaggccat tctgtcagcc
      721 cggctgtcaa agagccgcag acttgagaat cttatcgctc agctgccggg tgaaaagaaa
      781 aatggactgt tcgggaacct gattgctctt tcacttgggc tgactcccaa tttcaagtct
      841 aatttcgacc tggcagagga tgccaagctg caactgtcca aggacaccta tgatgacgat
      901 ctcgacaacc tcctggccca gatcggtgac caatacgccg accttttcct tgctgctaag
      961 aatctttctg acgccatcct gctgtctgac attctccgcg tgaacactga aatcaccaag
     1021 gcccctcttt cagcttcaat gattaagcgg tatgatgagc accaccagga cctgaccctg
     1081 cttaaggcac tcgtccggca gcagcttccg gagaagtaca aggaaatctt ctttgaccag
     1141 tcaaagaatg gatacgccgg ctacatcgac ggaggtgcct cccaagagga attttataag
     1201 tttatcaaac ctatccttga gaagatggac ggcaccgaag agctcctcgt gaaactgaat
     1261 cgggaggatc tgctgcggaa gcagcgcact ttcgacaatg ggagcattcc ccaccagatc
     1321 catcttgggg agcttcacgc catccttcgg cgccaagagg acttctaccc ctttcttaag
     1381 gacaacaggg agaagattga gaaaattctc actttccgca tcccctacta cgtgggaccc
     1441 ctcgccagag gaaatagccg gtttgcttgg atgaccagaa agtcagaaga aactatcact
     1501 ccctggaact tcgaagaggt ggtggacaag ggagccagcg ctcagtcatt catcgaacgg
     1561 atgactaact tcgataagaa cctccccaat gagaaggtcc tgccgaaaca ttccctgctc
     1621 tacgagtact ttaccgtgta caacgagctg accaaggtga aatatgtcac cgaagggatg
     1681 aggaagcccg cattcctgtc aggcgaacaa aagaaggcaa ttgtggacct tctgttcaag
     1741 accaatagaa aggtgaccgt gaagcagctg aaggaggact atttcaagaa aattgaatgc
     1801 ttcgactctg tggagattag cggggtcgaa gatcggttca acgcaagcct gggtacctac
     1861 catgatctgc ttaagatcat caaggacaag gattttctgg acaatgagga gaaagaggac
     1921 atccttgagg acattgtcct gactctcact ctgttcgagg accgggaaat gatcgaggag
     1981 aggcttaaga cctacgccca tctgttcgac gataaagtga tgaagcaact taaacggaga
     2041 agatataccg gatggggacg ccttagccgc aaactcatca acggaatccg ggacaaacag
     2101 agcggaaaga ccattcttga tttccttaag agcgacggat tcgctaatcg caacttcatg
     2161 caacttatcc atgatgattc cctgaccttt aaggaggaca tccagaaggc ccaagtgtct
     2221 ggacaaggtg actcactgca cgagcatatc gcaaatctgg ctggttcacc cgctattaag
     2281 aagggtattc tccagaccgt gaaagtcgtg gacgagctgg tcaaggtgat gggtcgccat
     2341 aaaccagaga acattgtcat cgagatggcc agggaaaacc agactaccca gaagggacag
     2401 aagaacagca gggagcggat gaaaagaatt gaggaaggga ttaaggagct cgggtcacag
     2461 atccttaaag agcacccggt ggaaaacacc cagcttcaga atgagaagct ctatctgtac
     2521 taccttcaaa atggacgcga tatgtatgtg gaccaagagc ttgatatcaa caggctctca
     2581 gactacgacg tggacgccat cgtccctcag agcttcctca aagacgactc aattgacaat
     2641 aaggtgctga ctcgctcaga caagaaccgg ggaaagtcag ataacgtgcc ctcagaggaa
     2701 gtcgtgaaaa agatgaagaa ctattggcgc cagcttctga acgcaaagct gatcactcag
     2761 cggaagttcg acaatctcac taaggctgag aggggcggac tgagcgaact ggacaaagca
     2821 ggattcatta aacggcaact tgtggagact cggcagatta ctaaacatgt agcccaaatc
     2881 cttgactcac gcatgaatac caagtacgac gaaaacgaca aacttatccg cgaggtgaag
     2941 gtgattaccc tgaagtccaa gctggtcagc gatttcagaa aggactttca attctacaaa
     3001 gtgcgggaga tcaataacta tcatcatgct catgacgcat atctgaatgc cgtggtggga
     3061 accgccctga tcaagaagta cccaaagctg gaaagcgagt tcgtgtacgg agactacaag
     3121 gtctacgacg tgcgcaagat gattgccaaa tctgagcagg agatcggaaa ggccaccgca
     3181 aagtacttct tctacagcaa catcatgaat ttcttcaaga ccgaaatcac ccttgcaaac
     3241 ggtgagatcc ggaagaggcc gctcatcgag actaatgggg agactggcga aatcgtgtgg
     3301 gacaagggca gagatttcgc taccgtgcgc aaagtgcttt ctatgcctca agtgaacatc
     3361 gtgaagaaaa ccgaggtgca aaccggaggc ttttctaagg aatcaatcct ccccaagcgc
     3421 aactccgaca agctcattgc aaggaagaag gattgggacc ctaagaagta cggcggattc
     3481 gattcaccaa ctgtggctta ttctgtcctg gtcgtggcta aggtggaaaa aggaaagtct
     3541 aagaagctca agagcgtgaa ggaactgctg ggtatcacca ttatggagcg cagctccttc
     3601 gagaagaacc caattgactt tctcgaagcc aaaggttaca aggaagtcaa gaaggacctt
     3661 atcatcaagc tcccaaagta tagcctgttc gaactggaga atgggcggaa gcggatgctc
     3721 gcctccgctg gcgaacttca gaagggtaat gagctggctc tcccctccaa gtacgtgaat
     3781 ttcctctacc ttgcaagcca ttacgagaag ctgaagggga gccccgagga caacgagcaa
     3841 aagcaactgt ttgtggagca gcataagcat tatctggacg agatcattga gcagatttcc
     3901 gagttttcta aacgcgtcat tctcgctgat gccaacctcg ataaagtcct tagcgcatac
     3961 aataagcaca gagacaaacc aattcgggag caggctgaga atatcatcca cctgttcacc
     4021 ctcaccaatc ttggtgcccc tgccgcattc aagtacttcg acaccaccat cgaccggaaa
     4081 cgctatacct ccaccaaaga agtgctggac gccaccctca tccaccagag catcaccgga
     4141 ctttacgaaa ctcggattga cctctcacag ctcggagggg atgagggagc tcccaagaaa
     4201 aagcgcaagg taggtagttc cggatccggt tcttgagacc agaccaataa aaaacgcccg
     4261 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     4321 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt
     4381 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     4441 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     4501 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     4561 cccagggatt ggctgaaacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     4621 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     4681 cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     4741 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     4801 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     4861 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     4921 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     4981 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     5041 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     5101 ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5581 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctg
//
