LOCUS       sequence-267042-        7977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Red tagged macrophages.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Oehlers SH, Cronan MR, Scott NR, Thomas MI, Okuda KS, Walton EM, 
            Beerman RW, Crosier PS, Tobin DM
  TITLE     Interception of host angiogenic signalling limits mycobacterial 
            growth.
  JOURNAL   Nature. 2015 Jan 29;517(7536):612-5. doi: 10.1038/nature13967. Epub 
            2014 Nov 24.
  PUBMED    25470057
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13967"; journalName: "Nature"; date: "2015-01-29- 
            29"; volume: "517"; issue: "7536"; pages: "612-5"
COMMENT     Sequence Label: pDEST mpeg1:tomato-CAAX
FEATURES             Location/Qualifiers
     source          1..7977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..45
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    162..183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(198..203,204..221,222..228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    236..252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     241..263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     260..276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        297..315
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     352..368
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(402..413,414..601)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    1040..1174
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1190..1206
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1214..1234
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    1297..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1433..1457
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1525..2952)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(2958..2982)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2982..3000
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3037..3053
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4970..4989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4971..4989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4991..5011)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5031..5047)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(5420..5582,5583..5594)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     5943..5964
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6127..6231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6232..6300,6301..7092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6450..6469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7263..7851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7752..7771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
       61 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      121 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
      181 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      241 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
      301 aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg ctctagaact
      361 agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg
      421 agtaatttta cttgattact gtacttaagt attatttttg gggattttta ctttacttga
      481 gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag aaaaaaaagt
      541 actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt ggagatcact
      601 tcattctatt ttcccttgct attaccaaac caattgaatt gcgctgatgc ccagtttaat
      661 ttaaatgtta tttattctgc ctatgaaaat cgttttcaca ttatatgaaa ttggtcagac
      721 atgttcattg gtcctttgga agtgacgtca tgtcacatct attaccacaa tgcacagcac
      781 cttgacctgg aaattaggga aattataaca gtcaatcagt ggaagaaaat ggaggaagta
      841 tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga tcaagagcac
      901 ccgtggccgt atcttcgcag atctgatcta gaggatcata atcagccata ccacatttgt
      961 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     1021 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     1081 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1141 caaactcatc aatgtatctt atcatgtctg gatcatcatc gatggtaccg taaaacgacg
     1201 gccagtgaat tatcaactat gtataataaa gttggaaaaa acctcccaca cctccccctg
     1261 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cccccacttt
     1441 gtacaagaaa gctgggtaga tctaggagag cacacacttg cagctcatgc agccggggcc
     1501 actctcatca ggagggttca gcttcttgta gagctcgtcc atgccgtaca ggaacaggtg
     1561 gtggcggccc tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt
     1621 gatgtccagc ttggtgtcca cgtagtagta gccgggcagt tgcacgggct tcttggccat
     1681 gtagatggtc ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggcctggtg
     1741 gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc
     1801 catggtcttc ttctgcatta cggggccgtc gggggggaag ttggtgccgc gcatcttcac
     1861 cttgtagatc agcgtgccgt cctgcaggga ggagtcctgg gtcacggtca ccagaccgcc
     1921 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
     1981 atcggggatg tcggcggggt gcttcacgta cgccttggag ccgtacatga actgggggga
     2041 caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct tggcggtctg
     2101 ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcat
     2161 ggagccctcc atgcgcacct tgaagcgcat gaactctttg atgacggcca tgttgttgtc
     2221 ctcggaggag gcggtgccgg agctgccgct gccggtgctg ccggtgccat gccccaggaa
     2281 caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg
     2341 ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagttgca cgggcttctt
     2401 ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc
     2461 ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc
     2521 ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcgcat
     2581 cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccag
     2641 accgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
     2701 cttgtaatcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt acatgaactg
     2761 gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc
     2821 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
     2881 gttcatggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga cctcctcgcc
     2941 cttgctcacc atggtccagc ctgctttttt gtacaaactt gaattaaccc tcactaaagg
     3001 gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcccccggg
     3061 ctgcaggaat tcgatatcaa gctttgtttt gctgtctcct gcactaatgt gattttcaat
     3121 caaatatggt gattatagat tttgctgagt tcctcaataa aaccacttct cagtttcaaa
     3181 tccgtggggg aagggtcatg agactatgag gttcctcttt gtgttttggt accttgcgtt
     3241 caaacgttaa gttgaaggac aatttcatga agtgaatgaa tcaaatatat ttatattttt
     3301 tgtgaagtat gatctgttca ataatagatt tatatttaaa gctacgaagg attttgatga
     3361 gtcttcagaa tggttttgat gacgcaagag actgtgcgtt ttaaattttc cattggcttt
     3421 gaaagaggag gaattgaaat tggttggctt cttgttttag aaatgtacaa gattagcagg
     3481 tttttcaaat cctctttatt ataagcatct cctaaaactg ctgtgagcat aggggatcat
     3541 gaaagcccta tagcagaatt ttcttcaaaa aaataagtcg gtacaacaat gaatccttat
     3601 ttcctttcac tttttttgtt ctctccactt ttaaaaaaac atcagctgca ctgacattca
     3661 attatttttc agtaaaaaca tttagttggg ttaacataag attattagtt ttctggagag
     3721 gtgaactact ctgtaacaaa actttttaag ttgtgccaac tgaacatttt ttgtctgcag
     3781 aactggaatg cctgattttg agtgaacaaa aaaaaacata atccttatat ccttagattt
     3841 ttcttttgtg cttaactttt ttcaccttta attgacacaa cagtagagag tatagacagg
     3901 aaagcatggg gagagaggca ggattagcaa aggacatcga tgtgggaatc aaaccagcat
     3961 cagtgtgaac actggagtgc cttgtgtcaa cacactaatc actacaccat tggtgttgac
     4021 atgcatacaa cctttgtaaa catcagttgc actgacctaa aatatctgag gttgagtgaa
     4081 caaaaaactg tctttgtcct gccatgtttt gatccttgtc ttgtttatca ttattgtttt
     4141 acctcctgtc cggtttctaa ttctgtttct aataattgtt ttgttaacat ttgtctctgt
     4201 tttgtttgtt gtgataatgt attatttctc agtaccaatt tccacaagag ttttttgttc
     4261 actcaactga tgttttcaag taaatgttgg agttgatggt gtagtggtta gtgtgttgac
     4321 acctggcact ccggtgttca tagtgacctg agtttgattc ctacctcctg gttctatgct
     4381 catccctctc ctctcccaac actttcctgt ccatactccc tactgtcgtt tcaaataaag
     4441 gtgaaaaaaa tgttaagtac acaagaaaaa acaaaaggaa ataaggtttg tttgtttttt
     4501 tgttcactca tcaagcattg tagttctgct gataaaaatg tttagttggc acaacttaaa
     4561 aaggtttctt acagagtagt tcacctctcc agaaaactaa taatcttatg ttaacctaac
     4621 taagtgtttt tgtattactg acatactgta taattttagg tcagtgcagt tgatgtttcc
     4681 aaaagttgag tgaacaagaa aaagcaaaag gaaatatgct ttttgtagtt gttgttgttg
     4741 ttgttgtgct ggattaagtt attttttgtg tgccagcaca gttgtataat ttgtaatatt
     4801 aaagtactgc aaaacttact gtaaattatt ggcaataaat tgtcatattt taaaggattc
     4861 ttgttaagta aattttgtaa tagctgtaaa aacatttctg ctgctttcaa ataatttcag
     4921 atgtcagatg tgctccaaca actcgagggg gggcccggta cccaattcgc cctatagtga
     4981 gtcgtattac caacttttct atacaaagtt gatagcttgg cgtaatcatg gtcatagctg
     5041 tttcctgctc gagggcccat ctggcctgtg tttcagacac cagggagtct ctgctcacgt
     5101 ttcctgctat ttgcagcctc tctatcaaga ctaatacacc tcttcccgca tcggctgcct
     5161 gtgagaggct tttcagcact gcaggattgc ttttcagccc caaaagagct aggcttgaca
     5221 ctaacaattt tgagaatcag cttctactga agttaaatct gaggttttac aactttgagt
     5281 agcgtgtact ggcattagat tgtctgtctt atagtttgat aattaaatac aaacagttct
     5341 aaagcaggat aaaaccttgt atgcatttca tttaatgttt tttgagatta aaagcttaaa
     5401 caagaatctc tagttttctt tcttgctttt acttttactt ccttaatact caagtacaat
     5461 tttaatggag tactttttta cttttactca agtaagattc tagccagata cttttacttt
     5521 taattgagta aaattttccc taagtacttg tactttcact tgagtaaaat ttttgagtac
     5581 tttttacacc tctgtcaaga actcctggac aaacctctga cctgtgtgga acagagtgga
     5641 tatgggtgtc tgaacagata ttcacgtctt ttgcagatca gagggcattt ctggtgttgt
     5701 gcaatgacct gggtccaaca ggtaggcgta ctcgtatcca agagcattgt tggacaggag
     5761 gtagtctcca ttcctataaa gaggaaggaa tggcaccatg tagtagccgt cattgtggcc
     5821 aattggtgca ttggtacgtg ggtagatgga ccggggaggc tgatgagttc ttagccagcg
     5881 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5941 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6001 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6061 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     6481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatg
     7201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7381 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     7921 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgatacc
//
