LOCUS       sequence-267093-        8513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP013Env
FEATURES             Location/Qualifiers
     source          1..8513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        538..741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     692..712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     786..805
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        786..804
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3457..3483
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3934..3975
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3985..4002
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4025..4042)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4031..4255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4301..4729
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4388..4407)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4598..4619
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4738..4758)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4743..5072
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4923..5058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4985..5004
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5139..5933
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5193..5212)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5803..5822
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6109..6230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6146..6165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6200..6219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6279..6295)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6279..6295)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6292..6314)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6303..6319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6327..6333,6334..6351,6352..6357))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6372..6393
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6510..6527)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6681..7269)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6761..6780)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7440..8231,8232..8300))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8063..8082
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8301..8405)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8480..8499)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
       61 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
      121 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      541 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      661 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      721 gaggtctata taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc
      781 gaaattaata cgactcacta tagggagacc caagctggct agttaagctt ggtaccgagc
      841 tcggatccag tacccttcac cgatcaagct ttaggcatct cctatggcag gaagaagcgg
      901 agacagcgac gcagacctcc tcagggctgt cagattgatc aagcgcctct atcaaagcag
      961 taagtagtac atgtaatgta tcttttagca ataacaatag tagcattagt aatagtagca
     1021 ataatagcaa tagttgtgtg gtctatagta ttcatagaat ataggaaaat attaagacaa
     1081 aggaaaatag acaggttaat tgatagaata agagaaagag aagaagacag tggcaatgag
     1141 agtgaagggg atcaggagga gttatccaga attatggaaa gggggcatct tgttcctggg
     1201 aatgttgatg atctgtagtg ctacagaaaa gttgtgggtc acagtctatt atggggtacc
     1261 tgtgtggaaa gaagcaaacg ccactctatt ctgtgcatca gatgccaaag catatgataa
     1321 agaggtgcat aatgtttggg cctcacatgc ctgtgtacct acagacccgg acccacaaga
     1381 aatgttattg gaaaatgtga cagaaccttt taacatatgg aaaaatgaca tggtagaaca
     1441 gatgcatgag gatataatca gtttatggga tcaaagccta aaaccatgtg taaaattgac
     1501 cccactctgt gtcactttaa attgcagtca tagggtgaat actactaata tcgctaatag
     1561 tactactgag ggcaatagta gtaatataga agggggagaa gggataaaga actgctcttt
     1621 taatattacc acaaatataa gacaaaaggt gcaaaaagaa tatgcattgt tttattcaat
     1681 tgatgtagta ccaatagaaa atgataactc tagctatatg ataagaagtt gtaatacctc
     1741 agtcattaca caggcctgtc caaaaatatc ttttgagcca attcccatac attattgtgc
     1801 cccggctggt tttgcgattc taaaatgtaa agataagaag ttcagtggaa aaggaaattg
     1861 tacaaatgtc agcacagtac aatgtacaca tggaattaaa ccagtagtat caactcaatt
     1921 gctgttaaat ggcagtctag cagaagaaga ggtggtaatt agatctgaca atttctcaaa
     1981 caatgttaaa aacatactag tacagctgaa ggagtctgta gaaattatct gtacaaggcc
     2041 caacaacaat acaagaaaaa gtatacatat aggaccaggg agagcatttt atgcaacagg
     2101 agaaataata ggaaacataa gacaggccca ttgtaacatt agtgaagcag actggaacca
     2161 gaccttaaaa gaagtagctg aaaaattaag agaacaattt cctaaccgca caatagtctt
     2221 taatcactcc tcaggagggg acccagaaat tgtaatgcac tcttttaatt gtggagggga
     2281 atttttctac tgtgatacaa caaaactgtt taacagtact tggcccaata acagtacttg
     2341 gcccaataac actgaagtca caaataccac tgaaaatggc actatcacac tccaatgcaa
     2401 aataaaacaa attataaacc tgtggcaaga agtaggaaaa gcaatgtatg cccctcccat
     2461 caatggaccg attaaatgta gatcaaatat tacagggtta atattaacaa gagatggtgg
     2521 tataaacaac accaccgaga ctaacaccac cgagactttt agacctggag gaggagatat
     2581 gagggacaat tggagaagtg aattatataa atacaaagta gtaagaattg aaccattagg
     2641 aatagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtggggct
     2701 gggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     2761 ggcgctgacg gtacaagcca gacaattatt gtctggtata gtgcagcagc agaacaatct
     2821 gctgagggct attgaggcgc aacagcatct gttgcaactc actgtctggg gtatcaagca
     2881 gctccaggca agagtcctgg ctgtggaaag atacctacag gatcaacagc tcctggggat
     2941 ttggggctgc tctggaaaac tcatctgcac cactaatgtg ccttggaata ctagctggag
     3001 taataaatct ttggaaaaaa tttggaataa catgacttgg atgcagtggg ataaagaagt
     3061 tagtaactac acaagcataa tatacacctt aattgaagaa tcgcagatcc aacaagagaa
     3121 gaatgaacaa gacttattgg cattagataa atgggacagt ttgtggaatt ggtttaacat
     3181 atcaaactgg ctgtggtata taaaaatatt cataatgata gtaggaggct tggtaggttt
     3241 aagaatagtc ttttctgtac ttactatagt aaagagagtt aggcagggat attcaccatt
     3301 atcgtttcag acccaccgcc cagcccagag ggaacccgac aggcccgaag gaatcgaaga
     3361 cgaaggtgga gagagagaca gagacaggtc accaccttta gtggatggat tcttgacact
     3421 tatctgggtc gacctgagga gcctgttcct cttcagctac caccgcttga gagacttact
     3481 cttgattgta acgaggattg tggagactct gggacgcagg gggtgggaac tcctcaagta
     3541 ctggtggaac cttctacaat attggattca ggaattaaag aatagtgctg ttagcttgct
     3601 caacgccaca gctatagcag tagctgaggg gacagatagg tttatagaaa tattacaaag
     3661 ggcttataga gcttttcgta acatccctgt aagaataaga caaggtttag aattggcttt
     3721 gctataagat gggtggcaag ggatcaaaga gctgtatgcc tggatgggct gctgtaaggg
     3781 aaagaatgag acgagctgac ccggcagcag aaggggcggc agcagaaggg gtgggagcag
     3841 tatctcgaga cggatccagc taagggtcaa gacaattctg cagatatcca gcacagtggc
     3901 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     3961 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
     4021 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4081 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4141 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4201 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4261 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4321 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4381 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4441 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4501 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4561 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4621 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4681 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     4741 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4801 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4861 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     4921 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     4981 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5041 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5101 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5161 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5221 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5281 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5341 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5401 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5461 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5521 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5581 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5641 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     5701 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5761 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5821 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     5881 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     5941 tctggggttc gcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6001 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6061 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6121 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6181 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6241 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6301 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6361 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6421 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6481 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6541 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6601 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6661 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6721 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6781 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     6841 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     6901 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     6961 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7021 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7081 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7141 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7201 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7261 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7321 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7381 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7441 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7501 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7561 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7621 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7681 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7741 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7801 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7861 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     7921 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     7981 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8041 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8101 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8161 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8221 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8281 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8341 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8401 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     8461 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cag
//
