LOCUS       sequence-267096-        8468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP024Env
FEATURES             Location/Qualifiers
     source          1..8468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        148..527
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        528..731
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     682..702
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     776..795
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        776..794
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2655..2888
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3879..3920
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3930..3947
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3970..3987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3976..4200
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4246..4674
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4333..4352)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4543..4564
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4683..4703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4688..5017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4868..5003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4930..4949
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5084..5878
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5138..5157)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5748..5767
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6054..6175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6091..6110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6145..6164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6224..6240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6224..6240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6237..6259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6248..6264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6272..6278,6279..6296,6297..6302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6317..6338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6455..6472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6626..7214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6706..6725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7385..8176,8177..8245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8008..8027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8246..8350)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8425..8444)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
       61 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      121 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      181 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      241 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      301 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      361 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      421 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      481 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      541 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      601 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      661 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      721 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
      781 cgactcacta tagggagacc caagctggct agttaagctt ggtaccgagc tcggatccag
      841 tacccttcac cgatcaagct ttaggcatct cctatggcag gaagaagcgg agacagcgac
      901 gaagaactcc tcaagacagt caggctcatc aaggatctct atcaaagcag tgagtagtac
      961 atgtaatgca acccttagtt atactagcaa tagtagcttt agtaataaca gcaatattag
     1021 caataattgt gtggactata gtgcttatag aatataggaa aatattaaga caaaggaaaa
     1081 tagacaggtt aattgataga atagcagaaa gagcagaaga cagtggcaat gagagtgaag
     1141 gggaccaaga agaattatca gctcttgtgg agaggggtca tcatgctcct tggaatgttg
     1201 atgatctgta gtgcagaaca attgtgggtt acagtctatt atggggtacc tgtgtggaaa
     1261 gaagcaacca ccactctatt ctgtgcatca gatgctaaag catatgacac agaggtacat
     1321 aatgtttggg ctacacatgc ctgtgtaccc acagacccta gcccacaaga agtacacttg
     1381 aaaaatgtga cagaagaatt taacatgtgg aagaatgaca tggcagaaca aatgcatgag
     1441 gatgtaatca gtttatggga tcaaagccta aagccatgtg taaaattaac cccactctgt
     1501 gtcactttaa actgcactga atattatggt cagagtcaga atgagactaa caataggatg
     1561 gaaaatgtaa ctgaatacaa aaactgctct ttcaatgtca ccacaagtag aagagataag
     1621 atgcagaaag aatatgcaat ttttactaag tatgatgtag taaagtcaga tgataataca
     1681 agctatacat tgataaattg taacacctca gtcattacac aggcctgtcc aaaggtaacc
     1741 tttgaaccaa ttcccataca ttattgtgcc ccggctggtt ttgcgcttct aaaatgtaat
     1801 gataagagat tcaatggaac aggagcatgt ccaaatgtca gcacagtaca atgtacacat
     1861 ggaattaggc cagtagtgtc aactcaactg ctgttaaatg gcagtctagc agaagaagaa
     1921 ataataatta gatctgaaaa tctcacaaac aatgctaaaa ccataatagt acatctaaat
     1981 gaaactgtag taattaattg tacaagacct aataacaata caagaaaaag tatacacttg
     2041 ggatggggga gaacaatgta tgcaacagga caaataatag gacaaataag acaagcacat
     2101 tgtaacatta gtaaaattaa atgggaaaac actttaagaa ggatagctat aaaattaaga
     2161 gaacaattta ataaaacaat agtctttaat aaatcctcag gaggggaccc agaaattgta
     2221 atgcacactt ttaattgtag aggagaattt ttctattgta atacaacacc gctgtttaat
     2281 agtagttgga atgaaactac tcttcagaag ggaaataata gtactcagga taatgaaact
     2341 atcacaatcc aatgcagaat aaaacaaatt ataaacaggt ggcaggaagt aggaaaagca
     2401 atgtatgccc ctcccatcca aggactaatt atgtgtgcat caaatattac agggatacta
     2461 ttaacaagag atggtggtga tggtaatggt acaaacaata ccaccgagat cttcagacct
     2521 ggaggaggag acatgaggga caattggaga aatgaattat ataaatataa agtagtacaa
     2581 attgagccat taggagtagc acccagcaag gcaaagagaa gagtggtgca aagagaaaaa
     2641 agagcagtag gactaggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     2701 ggcgcagcgt cgatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcaa
     2761 cagcagaaca atctgctgag ggctattgag gcgcaacaac agctgttgca actcacagtc
     2821 tggggcatta agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     2881 cagctcctag ggatttgggg ctgccatgga agacacattt gcaccactgc tgtgccttgg
     2941 aatactagtt ggagtaatag gtctctggat gttatttgga ataacatgac ttggatgcaa
     3001 tgggaaaggg aaattgaaaa ttacacaggc ctaatataca ccttaattga agagtcgcaa
     3061 aaccagcaag aaaagaatga aaaggaatta ttagaattgg ataagtgggc aagtttgtgg
     3121 agttggttta acatatcaaa ttggctgtgg tatataagat tattcataat gatagtagga
     3181 ggcttgatag gtttaagaat agtttttact gtgctttcta taataaatag agttaggcag
     3241 ggatactcac ccttgtcgtt ccagacccgc ctcccagcca ggaggggacc cgacaggccc
     3301 gaagaaatcg aagaaggagg tggagagaga gacagagaca catcagaacg attagtgacc
     3361 ggattctttg cgcttatctg ggacgatctc cggagcctgt tcctcttcag ctaccaccgc
     3421 ttgacagact tactcttgat tgcagcgagg attgtggaac ttctgggacg cagggggtgg
     3481 gaagccctca agtattggtg gaaccttctg cagtattgga gtcaggaact aaagagtagt
     3541 gctattagct tgtttaatgc tatagctatt gcagtagctg aggggacaga taggggttta
     3601 gaattagtac aaagattttg gagaggtatc ctccatatac ctagaagaat aagacagggc
     3661 ttggaaaggg ctttgatata agatgggtgg caagtggtca aaatggagtg ggtggtctaa
     3721 tgtaagggaa agaataagac gagctgagcc tcgagctgag ccagcagcag aaggggtggg
     3781 agcagtatct cgagacggat ccagctaagg gtcaagacaa ttctgcagat atccagcaca
     3841 gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     3901 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     3961 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4021 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4081 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4141 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4201 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     4261 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4321 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4381 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4441 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4501 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4561 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4621 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     4681 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4741 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4801 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4861 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4921 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     4981 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5041 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5101 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5161 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5221 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     5281 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5341 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5401 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5461 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5521 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5581 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     5641 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5701 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5761 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5821 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5881 gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5941 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6001 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6181 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6241 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6301 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6361 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6421 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7381 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7441 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7501 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7561 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7621 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7681 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7741 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7801 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7861 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7921 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7981 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8041 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8101 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8161 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8221 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8281 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8341 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     8401 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8461 tctgctcc
//
