LOCUS       sequence-267124-        5919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part Plasmid for Mxi1-dCas9, Part 3(coding sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Geller SH, Antwi EB, Di Ventura B, McClean MN
  TITLE     Optogenetic Repressors of Gene Expression in Yeasts Using 
            Light-Controlled Nuclear Localization.
  JOURNAL   Cell Mol Bioeng. 2019 Sep 24;12(5):511-528. doi: 
            10.1007/s12195-019-00598-9. eCollection 2019 Oct.
  PUBMED    31719930
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12195-019-00598-9"; journalName: "Cell Mol Bioeng"; date: 
            "2019-09-24- 24"; volume: "12"; issue: "5"; pages: "511-528"
COMMENT     Sequence Label: pMM729
FEATURES             Location/Qualifiers
     source          1..5919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..208
                     /codon_start=1
                     /product="transcriptional repressor domain from the mouse 
                     Max-interacting protein 1 (Schreiber-Agus et al., 1995)"
                     /label=Mxi1
                     /translation="MERVRMINVQRLLEAAEFLERRERECEHGYASSFPSMPSPR"
     terminator      complement(4359..4393)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(4464..5123)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     5038..5057
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      5305..5893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5794..5813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
       61 tgtggataac cgtagtcggt ctcatatgga acgtgtgaga atgattaatg tgcaaaggct
      121 gttagaagcc gcagagtttt tagaaagaag agaaagagaa tgcgaacacg ggtatgccag
      181 ttctttccct agcatgccct ctcccagagg ctccaagctt ggcggcatgg acaagaagta
      241 ttctatcgga ctggccatcg ggactaatag cgtcgggtgg gccgtgatca ctgacgagta
      301 caaggtgccc tctaagaagt tcaaggtgct cgggaacacc gaccggcatt ccatcaagaa
      361 aaatctgatc ggagctctcc tctttgattc aggggagacg gctgaagcaa cccgcctcaa
      421 gcggactgct agacggcggt acaccaggag gaagaaccgg atttgttacc ttcaagagat
      481 attctccaac gaaatggcaa aggtcgacga cagcttcttc cataggctgg aagaatcatt
      541 cctcgtggaa gaggataaga agcatgaacg gcatcccatc ttcggtaata tcgtcgacga
      601 ggtggcctat cacgagaaat acccaaccat ctaccatctt cgcaaaaagc tggtggactc
      661 aaccgacaag gcagacctcc ggcttatcta cctggccctg gcccacataa tcaagttcag
      721 aggccacttc ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg ataaactgtt
      781 catccagctg gtgcagactt acaaccagct ctttgaagag aaccccatca atgcaagcgg
      841 agtcgatgcc aaggccattc tgtcagcccg gctgtcaaag agccgcagac ttgagaatct
      901 tatcgctcag ctgccgggtg aaaagaaaaa tggactgttc gggaacctga ttgctctttc
      961 acttgggctg actcccaatt tcaagtctaa tttcgacctg gcagaggatg ccaagctgca
     1021 actgtccaag gacacctatg atgacgatct cgacaacctc ctggcccaga tcggtgacca
     1081 atacgccgac cttttccttg ctgctaagaa tctttctgac gccatcctgc tgtctgacat
     1141 tatccgcgtg aacactgaaa tcaccaaggc ccctctttca gcttcaatga ttaagcggta
     1201 tgatgagcac caccaggacc tgaccctgct taaggcactc gtccggcagc agcttccgga
     1261 gaagtacaag gaaatcttct ttgaccagtc aaagaatgga tacgccggct acatcgacgg
     1321 aggtgcctcc caagaggaat tttataagtt tatcaaacct atccttgaga agatggacgg
     1381 caccgaagag ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc agcgcacttt
     1441 cgacaatggg agcattcccc accagatcca tcttggggag cttcacgcca tccttcggcg
     1501 ccaagaggac ttctacccct ttcttaagga caacagggag aagattgaga aaattctcac
     1561 tttccgcatc ccctactacg tgggacccct cgccagagga aatagccggt ttgcttggat
     1621 gaccagaaag tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg tggacaaggg
     1681 agccagcgct cagtcattca tcgaacggat gactaacttc gataagaacc tccccaatga
     1741 gaaggtcctg ccgaaacatt ccctgctcta cgagtacttt accgtgtaca acgagctgac
     1801 caaggtgaaa tatgtcaccg aagggatgag gaagcccgca ttcctgtcag gcgaacaaaa
     1861 gaaggcaatt gtggaccttc tgttcaagac caatagaaag gtgaccgtga agcagctgaa
     1921 ggaggactat ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg gggtcgaaga
     1981 tcggttcaac gcaagcctgg gtacctacca tgatctgctt aagatcatca aggacaagga
     2041 ttttctggac aatgaggaga aagaggacat ccttgaggac attgtcctga ctctcactct
     2101 gttcgaggac cgggaaatga tcgaggagag gcttaagacc tacgcccatc tgttcgacga
     2161 taaagtgatg aagcaactta aacggagaag atataccgga tggggacgcc ttagccgcaa
     2221 actcatcaac ggaatccggg acaaacagag cggaaagacc attcttgatt tccttaagag
     2281 cgacggattc gctaatcgca acttcatgca acttatccat gatgattccc tgacctttaa
     2341 ggaggacatc cagaaggccc aagtgtctgg acaaggtgac tcactgcacg agcatatcgc
     2401 aaatctggct ggttcacccg ctattaagaa gggtattctc cagaccgtga aagtcgtgga
     2461 cgagctggtc aaggtgatgg gtcgccataa accagagaac attgtcatcg agatggccag
     2521 ggaaaaccag actacccaga agggacagaa gaacagcagg gagcggatga aaagaattga
     2581 ggaagggatt aaggagctcg ggtcacagat ccttaaagag cacccggtgg aaaacaccca
     2641 gcttcagaat gagaagctct atctgtacta ccttcaaaat ggacgcgata tgtatgtgga
     2701 ccaagagctt gatatcaaca ggctctcaga ctacgacgtg gacgccatcg tccctcagag
     2761 cttcctcaaa gacgactcaa ttgacaataa ggtgctgact cgctcagaca agaaccgggg
     2821 aaagtcagat aacgtgccct cagaggaagt cgtgaaaaag atgaagaact attggcgcca
     2881 gcttctgaac gcaaagctga tcactcagcg gaagttcgac aatctcacta aggctgagag
     2941 gggcggactg agcgaactgg acaaagcagg attcattaaa cggcaacttg tggagactcg
     3001 gcagattact aaacatgtag cccaaatcct tgactcacgc atgaatacca agtacgacga
     3061 aaacgacaaa cttatccgcg aggtgaaggt gattaccctg aagtccaagc tggtcagcga
     3121 tttcagaaag gactttcaat tctacaaagt gcgggagatc aataactatc atcatgctca
     3181 tgacgcatat ctgaatgccg tggtgggaac cgccctgatc aagaagtacc caaagctgga
     3241 aagcgagttc gtgtacggag actacaaggt ctacgacgtg cgcaagatga ttgccaaatc
     3301 tgagcaggag atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca tcatgaattt
     3361 cttcaagacc gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc tcatcgagac
     3421 taatggggag actggcgaaa tcgtgtggga caagggcaga gatttcgcta ccgtgcgcaa
     3481 agtgctttct atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa ccggaggctt
     3541 ttctaaggaa tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa ggaagaagga
     3601 ttgggaccct aagaagtacg gcggattcga ttcaccaact gtggcttatt ctgtcctggt
     3661 cgtggctaag gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg aactgctggg
     3721 tatcaccatt atggagcgca gctccttcga gaagaaccca attgactttc tcgaagccaa
     3781 aggttacaag gaagtcaaga aggaccttat catcaagctc ccaaagtata gcctgttcga
     3841 actggagaat gggcggaagc ggatgctcgc ctccgctggc gaacttcaga agggtaatga
     3901 gctggctctc ccctccaagt acgtgaattt cctctacctt gcaagccatt acgagaagct
     3961 gaaggggagc cccgaggaca acgagcaaaa gcaactgttt gtggagcagc ataagcatta
     4021 tctggacgag atcattgagc agatttccga gttttctaaa cgcgtcattc tcgctgatgc
     4081 caacctcgat aaagtcctta gcgcatacaa taagcacaga gacaaaccaa ttcgggagca
     4141 ggctgagaat atcatccacc tgttcaccct caccaatctt ggtgcccctg ccgcattcaa
     4201 gtacttcgac accaccatcg accggaaacg ctatacctcc accaaagaag tgctggacgc
     4261 caccctcatc caccagagca tcaccggact ttacgaaact cggattgacc tctcacagct
     4321 cggaggggat gagggagctg gatcctgaga ccagaccaat aaaaaacgcc cggcggcaac
     4381 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     4441 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     4501 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     4561 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     4621 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     4681 ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     4741 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     4801 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     4861 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     4921 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     4981 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     5041 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     5101 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     5161 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     5221 ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5281 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5341 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5401 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     5461 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     5521 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     5581 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     5641 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     5701 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5761 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5821 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5881 gagcctatgg aaaaacgcca gcaacgcggc ctttttacg
//
