LOCUS       sequence-267195-       10991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TPC1 with truncated N-terminus, tagged with mCherry on C terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Ruas M, Davis LC, Chen CC, Morgan AJ, Chuang KT, Walseth TF, Grimm 
            C, Garnham C, Powell T, Platt N, Platt FM, Biel M, Wahl-Schott C, 
            Parrington J, Galione A
  TITLE     Expression of Ca(2)(+)-permeable two-pore channels rescues NAADP 
            signalling in TPC-deficient cells.
  JOURNAL   EMBO J. 2015 Jul 2;34(13):1743-58. doi: 10.15252/embj.201490009. 
            Epub 2015 Apr 14.
  PUBMED    25872774
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201490009"; journalName: "EMBO J"; date: "2015-07-2- 
            2"; volume: "34"; issue: "13"; pages: "1743-58"
COMMENT     Sequence Label: TPC1(Delta-N69)-mCherry
FEATURES             Location/Qualifiers
     source          1..10991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    160..275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        333..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        637..840
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     791..811
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     837..861
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3222..3929
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNLAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3364..3382)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3632..3651
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3958..4457
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3993..4016)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4390..4409
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4478..5077
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4478..4497)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4974..4994
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5091..5679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5144..5164)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5562..5573)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5886..6519
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6648..6664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6648..6664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6661..6683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6672..6688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6696..6702,6703..6720,6721..6726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6741..6762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6879..6896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7050..7638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7130..7149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7809..8600,8601..8669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8432..8451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8670..8774)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8822..8956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8859..8878)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8913..8932
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9124..9757
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9804..9929
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10426..10659
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10844..10888
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
       61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      121 gatccattcg attagtgaac ggatctcgac ggtatcgcct ttaaaagaaa aggggggatt
      181 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      241 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      301 gatccagttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      841 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      901 agacaccgac tctactagag gatcgctagc gctaccggac tcagatctcg agctcaagct
      961 tcgaattcat gaattatcaa gaggcggcaa tctacctcca ggaaggtcag aacaacgaca
     1021 agttcttcac ccaccccaag gatgccagag cgctggcggc ctacctcttc gtccacaacc
     1081 acttcttcta catgatggag ctgctcacgg ccctgctcct gctgctgctg tcgctgtgcg
     1141 agtcccccgc tgtccccgtg ctcaagctgc acacttacgt ccacgccacg ctggaactct
     1201 ttgccctcat ggtggtggta tttgaactct gcatgaaatt gcggtggctg ggcttccaca
     1261 cgttcgtccg gcacaaacgt accatggtca agacgtccgt cctcgtggtg cagttcatcg
     1321 aggccattgt ggtgctggtt cggcagacgt cccacgtgcg ggtgacccgg gcactacgct
     1381 gcattttcct ggtggactgt cgctactgtg gcggtgtacg gcgcaacctg cggcagatct
     1441 tccagtctct cccacccttc atggacatcc tcctgttgct gctcttcttt atgatcatct
     1501 ttgccatcct cggtttctac ttattctcca caaatccttc cgacccctac ttcagcaccc
     1561 tggagaacag catcgtcaac ctgttcgttc tcctgaccac agccaacttt ccagatgtca
     1621 tgatgccctc ctactcccgg aacccctggt cctgcgtctt cttcattgta tacctctcca
     1681 ttgagctgta cttcatcatg aacctgctcc tggccgtggt gttcgacacc ttcaacgaca
     1741 ttgaaaagca caagttcaag tctttgctgc tgcacaaacg gaccgccatc cagcatgcct
     1801 acggcctgct tgccagccaa cggaggccgg ctggcatctc ctacaggcag ttcgaaggct
     1861 taatgcgctt ctacaagccc cggatgagtg caagggaacg cttcctgact ttcaaggcct
     1921 tgaaccagag caacacgcct ctgctcagcc tgaaggactt ctatgatatt tacgaagtcg
     1981 ctgctctgca gtggaaggca aagagaaaca gacagcattg gtttgatgag ctcccccgga
     2041 cagccttcct catcttcaaa gggattaaca tccttgtgaa ttccaaggcc ttccagtatt
     2101 tcatgtactt ggtggtggct gtcaacggtg tctggatcct ggtggagaca ttcatgttga
     2161 aaggtgggaa tttcacctca aagcatgtgc cctggagtta cctcgtgttt cttaccatct
     2221 atggagttga actgttcatg aaggtggctg gcctgggccc tgtggagtac ctgtcctctg
     2281 gatggaacct gttcgatttc tcggtcacgg cattcgcctt cctgggactg ctcgcactga
     2341 cgctcaacat ggaacccttc tatttcattg tggtcctgcg tccccttcag ctgctgaggt
     2401 tatttaaact gaagaaacgc taccgcaacg tgttggacac catgtttgag ctgctgccgc
     2461 ggatggccag ccttggcctc acgctgctca ccttctacta ttccttcgcc atcgtgggca
     2521 tggagttctt caacgggcgg ctgaccccca actgctgcaa caccagcaca gtggccgacg
     2581 cctaccggtt catcaaccac actgtgggca ataagaccaa ggtagaggaa ggctactact
     2641 atctcaacaa ctttgacaac atcctcaaca gcttcgtgac cttgtttgag ctcaccgttg
     2701 tcaacaattg gtacatcatc atggaaggcg tcacctcgca gacgtcccac tggagccgcc
     2761 tgtacttcat gaccttttac atagtgacca tggtggtgat gaccattatc gtggccttca
     2821 tcctggaggc cttcgtcttc cgcatgaact acagccgcaa gagccaggac tcggaagtgg
     2881 acagtggcat cgtcatcgag aaggaaatgt ccaaggagga gcttatggcc gtcctggagc
     2941 tttatcgtga ggagcgaggc acctcctctg acgtgacccg gctgctggac accctctctc
     3001 agatggagaa ataccagcaa aattccatgg tgtttctggg acggcgatcg agaaccaaaa
     3061 gtgacctgag tctgaagatg taccaggagg agatccagga gtggtacgag gagcatgccc
     3121 gggaacagga gcagcagaag ctcaggggca gcgtgcccgg ccctgcagcc cagcagcccc
     3181 ctggcagtcg ccagcgctcc cagactgtca ccgcggccgc ggtgagcaag ggcgaggagg
     3241 ataacttagc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3301 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3361 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3421 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     3481 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3541 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3601 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     3661 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     3721 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     3781 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     3841 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     3901 ccggcggcat ggacgagctg tacaagtagg gatcccgcga ctctagataa ttctaccggg
     3961 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     4021 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     4081 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     4141 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     4201 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     4261 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     4321 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     4381 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     4441 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt
     4501 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4561 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4621 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4681 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4741 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4801 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     4861 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     4921 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     4981 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5041 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc
     5101 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5161 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5221 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5281 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5341 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5401 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5461 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5521 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5581 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5641 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg
     5701 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5761 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5821 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag
     5881 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5941 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     6001 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     6061 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     6121 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     6181 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     6241 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     6301 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     6361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     6541 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt
     6601 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6661 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6721 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6781 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     7021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8821 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8881 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8941 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     9001 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     9061 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     9121 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     9181 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     9241 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     9301 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     9361 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     9421 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     9481 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     9541 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     9601 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9661 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9721 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9781 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9841 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9901 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9961 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    10021 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    10081 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    10141 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    10201 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    10261 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    10321 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10381 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    10441 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    10501 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    10561 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    10621 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    10681 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    10741 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    10801 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    10861 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    10921 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    10981 tgtactttct a
//
