LOCUS       sequence-267453-        8834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast expression of uTEV1-Delta fused to CRY2 and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Sanchez MI, Ting AY
  TITLE     Directed evolution improves the catalytic efficiency of TEV 
            protease.
  JOURNAL   Nat Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7.
  PUBMED    31819267
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2019 Dec 9. pii: 10.1038/s41592-019-0665-7. doi: 
            10.1038/s41592-019-0665-7."
COMMENT     Sequence Label: Gal:mCherry-CRY2-uTEV1-Delta(220-242)
FEATURES             Location/Qualifiers
     source          1..8834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      66..75
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             72..779
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(217..235)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     485..504
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3096..3343
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3112..3130)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3361..3380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3362..3380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3390..3407)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3390..3406)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3399..3421)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3547..4002
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3634..3653)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3844..3865
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(4262..4924)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(4925..5112)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(5259..5278)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5378..5400
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5438..5456)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5497..6000
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6037..6141
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6142..6210,6211..7002)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6360..6379)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7173..7761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7662..7681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7915..7932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8049..8070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8085..8090,8091..8108,8109..8115)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8123..8139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8128..8150
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8147..8163
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8147..8163
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8182..8202
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8184..8202
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8393..8834
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    8393..8510
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     8805..8828
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 aaaaaacccc ggatcgaatt ctacttcata cattttcaat taagatggct cgggatccac
       61 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
      121 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      181 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
      241 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
      301 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      361 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      421 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      481 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      541 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
      601 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      661 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      721 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
      781 ctctgggagg tcaaggtctg caggctagtg gtggaggagg ctctggtgga ggcggtagcg
      841 gaggcggagg gtcggctagc atgaagatgg acaaaaagac gattgtgtgg tttagacgtg
      901 accttcgcat tgaggacaac cctgcgttgg cagcggcagc gcatgaaggt tcggtattcc
      961 ctgtctttat ctggtgtccc gaagaggagg ggcaattcta tcccggtcgc gcgtcccgat
     1021 ggtggatgaa acagtcgctc gcacatctta gccagtcgtt gaaggcgctc ggctctgatc
     1081 tgacgctcat caaaacacac aatacaattt ctgccattct ggattgcatt agggtgactg
     1141 gtgccaccaa agttgtgttc aatcaccttt atgatcccgt aagcctggtc agggatcata
     1201 cagtaaaaga gaaacttgta gagaggggaa tcagcgtcca atcgtacaat ggggacttgt
     1261 tgtatgaacc gtgggaaatc tattgtgaga aaggcaaacc tttcacttcc ttcaattcgt
     1321 actggaagaa atgcctcgac atgtccatcg agtcagtcat gctgccgcca ccttggagac
     1381 ttatgcccat tactgctgcg gcagaggcga tctgggcttg cagcatcgaa gaacttggtc
     1441 tggaaaacga agcggaaaaa cccagtaacg cgctcctgac gcgagcgtgg tcgccaggct
     1501 ggtcgaatgc tgataagctc cttaatgagt ttatcgagaa acagttgatc gactatgcga
     1561 agaatagcaa gaaggtggtg ggaaattcca cttccctcct gtcgccgtat ctccactttg
     1621 gggagattag cgtgaggcat gtgttccaat gtgcgcggat gaagcaaatc atttgggctc
     1681 gcgacaaaaa ctctgaagga gaggagtctg ccgatttgtt tttgagagga atcggcctta
     1741 gggagtattc acgatacatc tgcttcaact ttccgtttac acatgaacaa tccctcctca
     1801 gccacctccg cttctttccg tgggacgcgg acgtggacaa attcaaggcc tggcggcagg
     1861 ggcgcacggg atacccgttg gtggatgcgg gtatgagaga gctgtgggca acgggttgga
     1921 tgcacaaccg tattcgagtc atcgttagta gctttgccgt taagttcctc ttgttgccct
     1981 ggaaatgggg gatgaaatac ttttgggaca cgcttctgga tgcggacttg gagtgcgata
     2041 ttcttgggtg gcagtacatt tcgggttcca tccctgacgg gcatgaactt gaccgattgg
     2101 ataaccctgc actgcaagga gcaaagtacg acccggaggg agaatacatc aggcagtggc
     2161 tcccagagct ggcccgtctg cccaccgagt ggattcacca cccgtgggat gctcccctga
     2221 ccgtccttaa agcctcaggg gtcgaattgg ggacgaatta cgcgaaaccc atcgtcgata
     2281 ttgacaccgc acgagaattg ctggcgaaag ccatctcaag aacacgcgaa gcgcagatta
     2341 tgatcggagc agcgccggga ggtagcggct ctggtggaag tggatcaggc agcggtggat
     2401 ctggcagcgg aaagcttggt tccgggggag aatccttgtt taagggacca cgtgattaca
     2461 acccgatatc gagcaccatt tgtcatttga cgaatgaatc tgatgggcac acaacatcgt
     2521 tgtatggtat tggatttggt cccttcatca ttacaaacaa gcacttgttt agaagaaata
     2581 atggaacact gttggtccaa tcactacatg gtgtattcaa ggtcaagaac accacgactt
     2641 tgcaacaaca cctcattgat gggagggaca tgataattat tcgcatgcct aaggatttcc
     2701 caccatttcc tcaaaagctg aaatttagag agccacaaag ggaagagcgc atatgtcttg
     2761 tgacaaccaa cttccaaact aagagcatgt ctagcatggt gtcagacact agttgcacat
     2821 tcccttcatc tgatggcata ttctggaagc attggattca aaccaaggat gggcagtgtg
     2881 gcaatccatt agtatcaact agagatgggt tcattgttgg tatacactca gcatcgaatt
     2941 tcaccaacac aaacaattat ttcacaagcg tgccgaaaaa cttcatggaa ttgttgacaa
     3001 atcaggaggc gcagcagtgg gttagtggtt ggcgattaaa tgctgactca gtattgtggg
     3061 ggggccataa agttttcatg gtgtaatagc tcgagtcatg taattagtta tgtcacgctt
     3121 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
     3181 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
     3241 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
     3301 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcggccggt acccaattcg
     3361 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3541 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3601 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3661 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3721 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3781 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3841 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3901 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3961 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
     4021 ccttacgcat ctgtgcggta tttcacaccg catagatccg tcgagttcaa gagaaaaaaa
     4081 aagaaaaagc aaaaagaaaa aaggaaagcg cgcctcgttc agaatgacac gtatagaatg
     4141 atgcattacc ttgtcatctt cagtatcata ctgttcgtat acatacttac tgacattcat
     4201 aggtatacat atatacacat gtatatatat cgtatgctgc aactttaaat aatcggtgtc
     4261 actacataag aacacctttg gtggagggaa catcgtttgt accattgggc gaggtggctt
     4321 ctcttatggc aaccgcaaga gccttgaacg cactctcact acggtgatga tcattcttgc
     4381 ctcgcagaca atcaacgtgg agggtaattc tgctagcctc tgcaaaactt tcaagaaaat
     4441 gcgggatcat ctcgcaagag agatctccta ctttctccct ttgcaaacca agttcgacaa
     4501 ctgcgtacgg cctgttcgaa agatctacca ccgctctgga aagtgcctca tccaaaggcg
     4561 caaatcctga tccaaacctt tttactccac gcacggcccc tagggcctct ttaaatgctt
     4621 gaccgagagc aatcccgcag tcttcagtgg tgtgatggtc gtctatgtgt aagtcaccaa
     4681 tgcactcaac gattagcgac cagccggaat gcttggccag agcatgtatc atatggtcca
     4741 gaaaccctat acctgtgtgg acgttaatca cttgcgattg tgtggcctgt tctgctactg
     4801 cttctgcctc tttttctggg aagatcgagt gctctatcgc taggggacca ccctttaaag
     4861 agatcgcaat ctgaatcttg gtttcatttg taatacgctt tactagggct ttctgctctg
     4921 tcatctttgc cttcgtttat cttgcctgct cattttttag tatattcttc gaagaaatca
     4981 cattacttta tataatgtat aattcattat gtgataatgc caatcgctaa gaaaaaaaaa
     5041 gagtcatccg ctaggtggaa aaaaaaaaat gaaaatcatt accgaggcat aaaaaaatat
     5101 agagtgtact agaggaggcc aagagtaata gaaaaagaaa attgcgggaa aggactgtgt
     5161 tatgacttcc ctgactaatg ccgtgttcaa acgatacctg gcagtgactc ctagcgctca
     5221 ccaagctctt aaaacgggaa tttatggtgc actctcagta caatctgctc tgatgccgca
     5281 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     5341 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     5401 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     5461 taggttaatg tcatgataat aatggtttct tagacggatc gcttgcctgt aacttacacg
     5521 cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt
     5581 atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag
     5641 aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt
     5701 tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta
     5761 aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta
     5821 atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt
     5881 cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat
     5941 ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat
     6001 gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6061 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6121 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6181 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6241 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6301 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6361 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6421 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6481 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6541 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6601 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6661 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6721 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6781 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6841 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6901 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6961 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7021 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7081 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7861 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7921 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     7981 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8041 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8101 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     8161 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
     8221 cgcaaagaag tttaataatc atattacatg gcattaccac catatacata tccatatcta
     8281 atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt
     8341 ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag
     8401 aagccgccga gcgggtgaca gccctccgaa ggaagactct cctccgtgcg tcctcgtctt
     8461 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag
     8521 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca
     8581 caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt
     8641 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata
     8701 tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt cggtttgtat
     8761 tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact
     8821 ttaacgtcaa ggag
//
