LOCUS       sequence-267546-        6612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express FLAG-tagged ODC2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Kory N, Uit de Bos J, van der Rijt S, Jankovic N, Gura M, Arp N, 
            Pena IA, Prakash G, Chan SH, Kunchok T, Lewis CA, Sabatini DM
  TITLE     MCART1/SLC25A51 is required for mitochondrial NAD transport.
  JOURNAL   Sci Adv. 2020 Oct 21;6(43):eabe5310. doi: 10.1126/sciadv.abe5310. 
            Print 2020 Oct.
  PUBMED    33087354
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-10-21"; volume: "6(43):eabe5310. doi"; pages: " 
            10.1126/sciadv.abe5310. Print 2020 Oct"
COMMENT     Sequence Label: pMXs_FLAG-ODC2
FEATURES             Location/Qualifiers
     source          1..6612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             57..473
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     403..425
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     441..457
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    483..857
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     795..811
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      886..895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             895..960
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1935..2500
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1950..2502
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2094..2111)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2321..2340
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2514..2909
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             3080..3549
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3679..3696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3850..4438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3930..3949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4602..5393,5394..5462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5225..5244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5463..5567)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             5597..6190
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6253..6610
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
       61 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      121 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      181 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
      241 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
      301 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
      361 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
      421 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
      481 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
      541 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
      601 agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg
      661 tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc
      721 tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag
      781 tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg
      841 gaccatcctc tagactgccg gatctagcta gttaattaag gatccgccac catggactac
      901 aaggaccacg acggtgacta caaggaccac gacatcgact acaaggacga cgacgacaag
      961 gggcatggca ccggcagcac cggcagcgaa ttcatgtcca gcgattccaa tgctaagcca
     1021 ttgcctttca tataccaatt tatatctgga gcggtagcgg gaattagcga actcacggtc
     1081 atgtacccgt tggacgttgt taaaacacgg tttcaattgg aagtaacaac cccgacagca
     1141 gcagcagtcg gaaaacaggt tgaacgctat aacggcgtta tagattgttt gaaaaaaatt
     1201 gtcaagaaag agggattctc ccgattgtat agaggtataa gcagtcccat gctcatggaa
     1261 gccccaaaac gggccactaa gtttgcctgc aatgatcagt accagaagat cttcaaaaat
     1321 ctttttaaca cgaatgagac cacacaaaaa atatccatag cagccggtgc aagcgcgggg
     1381 atgacagaag ctgccgttat tgtaccgttt gagctcatta agatacggat gcaagatgtt
     1441 aaatctagtt atctcgggcc gatggattgt ctcaaaaaga ccatcaagaa tgaaggtatc
     1501 atgggattgt acaaggggat agagtcaact atgtggagga acgccttgtg gaatggtgga
     1561 tattttggtg tcatctacca ggtgagaaat tctatgcccg ttgcgaagac gaaaggacaa
     1621 aaaactcgaa acgacttgat tgcgggcgct ataggcggca cagtcggcac aatgcttaac
     1681 acaccattcg acgtggtgaa atcacggata caatcagttg atgcagtgag tagtgccgtt
     1741 aagaagtaca attggtgtct cccaagcctt ctggttatat atcgggagga gggtttccga
     1801 gccctttaca aggggttcgt ccccaaagtg tgccggctgg ctccgggagg gtctctcatg
     1861 cttgtggttt ttacgggcat gatgaatttc tttagagatc tcaaatatgg gcattgagcg
     1921 gccgctacgt aaattccscc ccccccctaa cgttactggc cgaagccgct tggaataagg
     1981 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     2041 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     2101 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     2161 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     2221 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     2281 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     2341 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     2401 tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     2461 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggttc
     2521 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     2581 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     2641 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     2701 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     2761 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     2821 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     2881 tgccctctgg ttatgtgtgg gagggctaag tcgacgataa aataaaagat tttatttagt
     2941 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     3001 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
     3061 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     3121 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     3181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     3241 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     3301 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     3361 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     3421 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     3481 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     3541 ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     3601 taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc
     4561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     4621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     4681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     4741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     4801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     4861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     4921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     4981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     5521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     5581 aaagtgccac ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     5641 cgccattttg caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt
     5701 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     5761 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     5821 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     5881 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     5941 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     6001 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
     6061 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     6121 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     6181 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
     6241 ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat
     6301 gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac
     6361 ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga
     6421 cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct
     6481 ttggtgcacc cccctaatag gagggatatg tggttctggt aggagacgag aacctaaaac
     6541 agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc
     6601 ttgtctgctg ca
//
