LOCUS       sequence-267608-        8519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP043Env
FEATURES             Location/Qualifiers
     source          1..8519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        473..676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     627..647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     721..740
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        721..739
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3388..3414
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             3875..3916
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3926..3943
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3966..3983)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3972..4196
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4242..4670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4329..4348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4539..4560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4679..4699)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4684..5013
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4864..4999
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4926..4945
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5080..5874
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5134..5153)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5744..5763
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6050..6171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6087..6106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6141..6160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6220..6236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6220..6236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6233..6255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6244..6260
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6268..6274,6275..6292,6293..6298))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6313..6334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6451..6468)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6622..7210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6702..6721)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7381..8172,8173..8241))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8004..8023
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8242..8346)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8421..8440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
       61 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      481 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      601 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      661 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
      721 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      781 tccagtaccc ttcaccgatc aagctttagc atctcctatg gcaggaagaa gcggagacag
      841 cgacgaagac ctcctcaaga cagtcagact catcaagctt ctctatcaaa gcagtaagta
      901 atacatgtaa tgtacccttt acaaatatta gcaatagtag cattagtagt agcagcaata
      961 atagcaatag ttgtgtggtc catagtagtc atagaatata gaaaaatatt aagacaaaga
     1021 aaaatagaca ggttacttga tagaataata gaaagagcag aagacagtgg caatgagagt
     1081 gaaggggacc aagaagaatt gtcagcactt gtggagatgg ggcatcatgc tccttgggat
     1141 gttaatgatc tgtagtgctg gagaaaaaac gtgggtcaca gtctattatg gggtacctgt
     1201 gtggaaagag gcaaccacca ctctattttg tgcatcagat gctaaagcat atgatacaga
     1261 ggtgcataat gtttgggcca cacatgcctg tgtacccact gaccctaacc cacaagaaat
     1321 agttgtggga aatgtgacag aaaattttaa catgtggaaa aataacatgg tagaacagat
     1381 gcatgaggat ataatcagtc tatgggatca aagcctaaag ccatgtgtaa aactaacccc
     1441 attatgtgtt actttaaagt gtagtaatgc caatcttact actaataata ctatcaacaa
     1501 caacaacaag atggaggaag gagaaataaa aaattgctct ttcaatatca ccacagaact
     1561 gaaggataaa tatcagcaac aatatgcatt gttttataag cttgatatag tgccaataga
     1621 tgataataat aatactaata atgatagtag gaccagctat aggttaataa gttgtaacac
     1681 ctcagtcatt acacaggcct gcccaaaggt atcctttaaa ccaattccca tacattattg
     1741 tgccccagct ggttatgcaa ttctaaagtg taacaatgag gggttcaatg gaacaggacc
     1801 atgtacaaat gtcagctcag tacaatgtac acatggaatt aaaccagtag tatcaactca
     1861 actgctgtta aatggcagtc tagcaaaaga agaggtagta cttagatctg ccaacttcac
     1921 agacaatgct aagaacataa tagtacagct gaacaaatct gtagtaatta actgtacaag
     1981 gcccaacaac aatacaagaa aaagtatacc tatgggacca gggagagcat tctttacaac
     2041 agacataata ggagacataa gacaagcaca ttgtaatatt agtggtacag aatggaatga
     2101 cactctaaaa aaggtagttg aaaaattagg aaaacaattt ggggagaata caacgataat
     2161 ctttaataca tcctcaggag gggacccaga aattgtaatg cacagtttta attgtggagg
     2221 ggagttcttc tactgtaatt caacacaact gtttaatagt acttggataa atggtacttg
     2281 gacaaatggt actacaaata ccactggaga agataagatt atactcccat gcaggataaa
     2341 acaaattata aacttgtggc aggaagtagg aaaggcaatg tacgcccctc ccatcagagg
     2401 acaaattacc tgttcatcaa atattacagg gttactatta acaagagatg ggggtaataa
     2461 gaacgggagt aatgaggccg ttgagacctt cagacctgca ggaggagata tgagggacaa
     2521 ttggagaagt gaattataca aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2581 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggat taggagcttt
     2641 gttcattggg ttcttgggag ccgcaggaag cactatgggc gcagcgtcag tgacgctgac
     2701 ggtacaggcc agacaattat tgtctggtat agtgcaacag cagagcaatc tgctgagggc
     2761 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2821 aagagtcctg gcggtggaaa gattcctaac ggatcaacag ctcctgggga tttggggctg
     2881 ctctggaaaa ctcatctgca ccacttctgt tccttggaat actacttgga gtaatagatc
     2941 tgtagatgac atttggaata acatgacatg gatgcaatgg gataaagaaa ttagcaatta
     3001 cacaggctac atatactcct tacttggaga agcacatgtc caacaagaag tgaatgaaaa
     3061 agaattattg gaactaggta aatgggcaag cttgtggaat tggtttgaca tatcaaactg
     3121 gctgtggtat ataaaaatat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3181 ttttattgta ctttctttag taaatagagt taggcaggga tactcaccat tgtcatttca
     3241 gacccacctc ccagctcaga ggggacccga caggcccgga ggaatcgaag aagaaggtgg
     3301 agagaaagac agagacacat caaggcaatt agtggatgga ttcttcacaa ttatctggag
     3361 cgatctaagg agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt
     3421 gacgaggatt gtggaacttc tgggacgcag ggggtgggaa accctcaaat attggtggaa
     3481 tctcctgcaa tattggagtc aggaactaca caatagtgct gttagcttgc tcaacgccat
     3541 agccatagca gtaggtgagg gaacagatag gattttagaa gcactaagaa gagctggtag
     3601 agctattctc cacataccta caagaataag acagggcttg gagatagctt tgctgtaaag
     3661 atgggtggca aatggtcaaa gcgtagtatg ccaggatggg atactgtgag ggaaagaata
     3721 agacgaacta gagttgaacc agcagcagta gttgaaccag cagcagttgg ggtgggagca
     3781 gtatctcgag acggatccag ctaagggtca agacaattct gcagatatcc agcacagtgg
     3841 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     3901 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     3961 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4021 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4081 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4141 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4201 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4261 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4321 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4381 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4441 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4501 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4561 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4621 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     4681 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4741 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4801 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4861 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4921 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4981 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5041 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     5101 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     5161 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     5221 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     5281 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5341 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5401 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5461 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5521 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5581 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     5641 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5701 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5761 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5821 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5881 ctctggggtt cgcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5941 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6001 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6061 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6181 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     6301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     6361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     6421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7021 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8341 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     8401 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8461 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaac
//
