LOCUS       sequence-267626-        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for constitutive expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  AUTHORS   Fischer AD, Veronese-Paniagua DA, Swaminathan S, Kashima H, Rubin 
            DC, Madison BB
  TITLE     The oncogenic function of PLAGL2 is mediated via ASCL2 and IGF2 and 
            a Wnt-independent mechanism in colorectal cancer.
  JOURNAL   Am J Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022.
  PUBMED    37310750
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J 
            Physiol Gastrointest Liver Physiol. 2023 Jun 13. doi: 
            10.1152/ajpgi.00058.2022."
COMMENT     Sequence Label: BII-ChBtW-iresFUCCI
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(269..285)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(269..285)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1218..1227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1224..1619
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             1638..1691
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1701..1724
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(1737..1739,1740..1742,1743..2453)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(1782..1803)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2390..2411
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2484..2813
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    2818..3390
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2985..3002)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3212..3231
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3405..4055
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             3405..4055
                     /codon_start=1
                     /product="humanized monomeric Kusabira-Orange fluorescent 
                     protein"
                     /label=hmKO
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     CDS             4110..4382
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     misc_feature    4407..4995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4460..4480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4878..4889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5075..5209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5099..5118)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5153..5172
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5988..6050
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6180..6197)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6180..6196)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6189..6211)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6338..6793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6425..6444)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6635..6656
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6819..6923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6924..6992,6993..7784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7142..7161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7955..8543
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8444..8463
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8697..8714
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8831..8852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8867..8872,8873..8890,8891..8897)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8905..8921
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8910..8932
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8929..8945
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8929..8945
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   9436..9470
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg tgcatttagg
       61 acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg taagtgtcac
      121 tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc atgattatct tttacgtgac
      181 ttttaagatt taactcatac gataattata ttgttatttc atgttctact tacgtgataa
      241 cttattatat atatattttc ttgttataga tccactagtt ctagagggac agcccccccc
      301 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
      361 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
      421 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
      481 tgagcctgca gacacctggg gggatacggg gaaaaggcct ccaaggcctg caggagtcaa
      541 tgggaaaaac ccattggagc caagtacact gactcaatag ggactttcca ttgggttttg
      601 cccagtacat aaggtcaata gggggtgagt caacaggaaa gtcccattgg agccaagtac
      661 attgagtcaa tagggacttt ccaatgggtt ttgcccagta cataaggtca atgggaggta
      721 agccaatggg tttttcccat tactgacatg tatactgagt cattagggac tttccaatgg
      781 gttttgccca gtacataagg tcaatagggg tgaatcaaca ggaaagtccc attggagcca
      841 agtacactga gtcaataggg actttccatt gggttttgcc cagtacaaaa ggtcaatagg
      901 gggtgagtca atgggttttt cccattattg gcacatacat aaggtcaata ggggtgacta
      961 gtggagaaga gcatgcttga gggctgagtg cccctcagtg ggcagagagc acatggccca
     1021 cagtccctga gaagttgggg ggaggggtgg gcaattgaac tggtgcctag agaaggtggg
     1081 gcttgggtaa actgggaaag tgatgtggtg tactggctcc acctttttcc ccagggtggg
     1141 ggagaaccat atataagtgc agtagtctct gtgaacattc ggtttagtga acctgcaggt
     1201 cggccgccac gaccggtgcc accatggcca agcctttgtc tcaagaagaa tccaccctca
     1261 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     1321 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     1381 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     1441 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     1501 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     1561 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcg
     1621 ctagccttgg ctccggcgag ggcaggggaa gtcttctaac atgcggggac gtggaggaaa
     1681 atcccggccc actcggcatg gactacaagg acgacgatga caagctcgat agcggcatgg
     1741 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1801 acgtaaacgg ccacaagttc agcgtccgcg gcgagggcga gggcgatgcc accaacggca
     1861 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1921 tgaccacctt cggctacggc gtggcctgct tcagccgcta ccccgaccac atgaagcagc
     1981 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atctctttca
     2041 aggacgacgg tacctacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2101 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2161 tggagtacaa cttcaacagc cacaacgtct atatcacggc cgacaagcag aagaacggca
     2221 tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagcgtgcag ctcgccgacc
     2281 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2341 tgagccatca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2401 tggagttcgt gaccgccgcc gggattacac atggcatgga cgagctgtac aaggatatcc
     2461 atgcacactg gcggccgctc gagatgaatc ccagtatgaa gcagaaacaa gaagaaatca
     2521 aagagaatat aaagaatagt tctgtcccaa gaagaactct gaagatgatt cagccttctg
     2581 catctggatc tcttgttgga agagaaaatg agctgtccgc aggcttgtcc aaaaggaaac
     2641 atcggaatga ccacttaaca tctacaactt ccagccctgg ggttattgtc ccagaatcta
     2701 gtgaaaataa aaatcttgga ggagtcaccc aggagtcatt tgatcttatg attaaagaaa
     2761 atccatcctc tcagtattgg aaggaagtgg cagaaaaacg gagaaaggcg ctgtaagccc
     2821 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2881 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     2941 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     3001 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     3061 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     3121 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     3181 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     3241 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     3301 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     3361 gtggttttcc tttgaaaaac acgatgataa tggccacaga attcatggtg agcgtgatca
     3421 agcccgagat gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa
     3481 tcgagggtga gggcacaggc agaccttacg agggacatca ggagatgaca ctgcgcgtca
     3541 caatggccga gggcgggcca atgcctttcg ccttcgacct ggtgtcccac gtgttctgtt
     3601 acggccacag agtttttacc aagtacccag aagagatccc agactatttc aagcaggcct
     3661 ttcctgaggg cctgtcctgg gagaggtccc tggagttcga ggacggcggc tccgcctccg
     3721 tgagcgccca catcagcctg aggggcaaca ccttctacca caagtccaag ttcaccggcg
     3781 tgaacttccc cgccgacggc cccatcatgc agaaccagag cgtggactgg gagccctcca
     3841 ccgagaagat caccgccagc gacggcgtgc tgaagggcga cgtgaccatg tacctgaagc
     3901 tggagggcgg cggcaaccac aagtgccaga tgaagaccac ctacaaggcc gccaaggaga
     3961 tcctggagat gcccggcgac cactacatcg gccacaggct ggtgaggaag accgagggca
     4021 acatcaccga gcaggtggag gacgccgtgg cccactactc aatgttgcct tccctgggcg
     4081 atatccatgc acactggcgg ccgctcgagc ccagccccgc caggcccgca ctccgcgccc
     4141 cggcctccgc taccagtggc agccgcaagc gcgcccgccc gcccgccgcc cccggacgcg
     4201 accaggccag gccaccggcc cgcaggagac tgcggctgtc ggtggacgag gtttccagcc
     4261 ccagtacccc cgaggcccca gacatcccag cctgcccttc tccgggccag aagataaaga
     4321 aatccacccc ggcagcaggt cagccgcccc acctgacatc cgcgcaggac caggacacca
     4381 tctgagctag agttcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat
     4441 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4501 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4561 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4621 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4681 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4741 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4801 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4861 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4921 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4981 gggccgcctc cccgcctgga attaattctg gctaagcact tcgtggccgt cgatcgttta
     5041 aagggaggta gtgagtcgag atatcgatac tagtgatcca gacatgataa gatacattga
     5101 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     5161 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     5221 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     5281 aaacctctac aaatgtggta tggctgatta tgatcctcta gagggacagc ccccccccaa
     5341 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     5401 gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc ggggacagcc
     5461 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     5521 gcctgcagac acctgggggg atacggggaa aaagccatac caatggagct cacggggaca
     5581 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     5641 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     5701 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     5761 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagaatt cgataaaagt
     5821 tttgttactt tatagaagaa attttgagtt tttgtttttt ttaataaata aataaacata
     5881 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
     5941 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
     6001 tacacatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagc
     6061 tgcgtgttct gcagcgtgtc gagcatcttc atctgctcca tcacgctgta aaacacattt
     6121 gcaccgcgag tctgcccgtc ctccacgggt tcaaaaacgt gaatgaacga ggcgcgctca
     6181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6301 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6361 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6421 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6481 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6541 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6601 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6661 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6721 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6781 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6841 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6901 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6961 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7021 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7081 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7141 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7201 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7261 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7321 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7381 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7441 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7501 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7561 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7621 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7681 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     7741 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     7801 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     7861 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7921 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7981 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8041 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8101 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8161 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8221 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8281 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8341 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8401 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8461 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8521 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8581 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8641 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     8701 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     8761 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     8821 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     8881 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     8941 atgaccatga ttacgccaag cgcgcccgcc gggtaactca cggggtatcc atgtccattt
     9001 ctgcggcatc cagccaggat acccgtcctc gctgacgtaa tatcccagcg ccgcaccgct
     9061 gtcattaatc tgcacaccgg cacggcagtt ccggctgtcg ccggtattgt tcgggttgct
     9121 gatgcgcttc gggctgacca tccggaactg tgtccggaaa agccgcgacg aactggtatc
     9181 ccaggtggcc tgaacgaaca gttcaccgtt aaaggcgtgc atggccacac cttcccgaat
     9241 catcatggta aacgtgcgtt ttcgctcaac gtcaatgcag cagcagtcat cctcggcaaa
     9301 ctctttccat gccgcttcaa cctcgcggga aaaggcacgg gcttcttcct ccccgatgcc
     9361 cagatagcgc cagcttgggc gatgactgag ccggaaaaaa gacccgacga tatgatcctg
     9421 atgcagctag attaacccta gaaagatagt ctgcgtaaaa ttgacgcatg
//
