LOCUS       sequence-267767-        1629 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Krautler NJ, Yermanos A, Pedrioli A, Welten SPM, Lorge D, Greczmiel 
            U, Bartsch I, Scheuermann J, Kiefer JD, Eyer K, Menzel U, Greiff V, 
            Neri D, Stadler T, Reddy ST, Oxenius A
  TITLE     Quantitative and Qualitative Analysis of Humoral Immunity Reveals 
            Continued and Personalized Evolution in Chronic Viral Infection.
  JOURNAL   Cell Rep. 2020 Jan 28;30(4):997-1012.e6. doi: 
            10.1016/j.celrep.2019.12.088.
  PUBMED    31995768
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.12"; journalName: "Cell Rep"; date: 
            "2020-01-28- 28"; volume: "30"; issue: "4"; pages: "997-1012.e6"
COMMENT     Sequence Label: pcDNA3.1_GP1Fc Cl13
FEATURES             Location/Qualifiers
     source          1..1629
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..34
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1600..1617)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctggcttatc gaattaatac gactcactat agggagaccc aagctggcta gcaggatggg
       61 tcagattgtg acaatgtttg aggctctgcc tcacatcatc gatgaggtga tcaacattgt
      121 cattattgtg cttatcgtga tcacgggtat caaggctgtc tacaattttg ccacctgtgg
      181 gatattcgca ttgatcagtt tcctacttct ggctggcagg tcctgtggca tgtacggtct
      241 taagggaccc gacatttaca aaggagttta ccaatttaag tcagtggagt ttgatatgtc
      301 acatctgaac ctgaccatgc ccaacgcatg ttcagccaac aactcccacc attacatcag
      361 tatggggact tctggactag aattgacctt caccaatgat tccatcatca gtcacaactt
      421 ttgcaatctg acctctgcct tcaacaaaaa gacctttgac cacacactca tgagtatagt
      481 ttcgagccta cacctcagta tcagagggaa ctccaactat aaggcagtat cctgcgactt
      541 caacaatggc ataaccatcc aatacaactt gacattctca gatgcacaaa gtgctcagag
      601 ccagtgtaga accttcagag gtagagtcct agatatgttt agaactgcct tcggggggaa
      661 atacatgagg agtggctggg gctggacagg ctcagatggc aagaccacct ggtgtagcca
      721 gacgagttac caatacctga ttatacaaaa tagaacctgg gaaaaccact gcacatatgc
      781 aggtcctttt gggatgtcca ggattctcct ttcccaagag aagactaagt tcctcactag
      841 gagactagcg ctgcaggagc ccaaatcttg tgacaaaact cacacatgtc caccgtgccc
      901 agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac
      961 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     1021 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     1081 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     1141 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     1201 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac
     1261 cctgccccca tcccgggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa
     1321 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     1381 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct atagcaagct
     1441 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     1501 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta aatgagcggc
     1561 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtg
     1621 ccagccatc
//
