LOCUS       sequence-267775-        9316 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CTCF-GFP in mammalian cells through transient 
            transfection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9316)
  AUTHORS   Wang AJ, Han Y, Jia N, Chen P, Minden MD
  TITLE     NPM1c impedes CTCF functions through cytoplasmic mislocalization in 
            acute myeloid leukemia.
  JOURNAL   Leukemia. 2020 May;34(5):1278-1290. doi: 10.1038/s41375-019-0681-8. 
            Epub 2019 Dec 12.
  PUBMED    31831844
REFERENCE   2  (bases 1 to 9316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41375-019-0681-8"; journalName: "Leukemia"; date: 
            "2020-05"; volume: "34"; issue: "5"; pages: "1278-1290"
COMMENT     Sequence Label: pAcGFP-Hyg-CTCF-N1
FEATURES             Location/Qualifiers
     source          1..9316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2866..3585
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    3707..3828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3798..3817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3835..4290)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3972..3993)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4184..4203
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4317..4421
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4423..4780
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4423..4614
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4446..4466)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4631..4766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4788..5813
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4788..5813
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5930..6011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6039..6057)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6125..6229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7568..7636,7637..8428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7786..7805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8614..9202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9103..9122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gaagctttta tggaaggtga tgcagtcgaa gccattgtgg aggagtccga aacttttatt
      721 aaaggaaagg agagaaagac ttaccagaga cgccgggaag ggggccagga agaagatgcc
      781 tgccacttac cccagaacca gacggatggg ggtgaggtgg tccaggatgt caacagcagt
      841 gtacagatgg tgatgatgga acagctggac cccacccttc ttcagatgaa gactgaagta
      901 atggagggca cagtggctcc agaagcagag gctgctgtgg acgataccca gattataact
      961 ttacaggttg taaatatgga ggaacagccc ataaacatag gagaacttca gcttgttcaa
     1021 gtacctgttc ctgtgactgt acctgttgct accacttcag tagaagaact tcagggggct
     1081 tatgaaaatg aagtgtctaa agagggcctt gcggaaagtg aacccatgat atgccacacc
     1141 ctacctttgc ctgaagggtt tcaggtggtt aaagtggggg ccaatggaga ggtggagaca
     1201 ctagaacaag gggaacttcc accccaggaa gatcctagtt ggcaaaaaga cccagactat
     1261 cagccaccag ccaaaaaaac aaagaaaacc aaaaagagca aactgcgtta tacagaggag
     1321 ggcaaagatg tagatgtgtc tgtctacgat tttgaggaag aacagcagga gggtctgcta
     1381 tcagaggtta atgcagagaa agtggttggt aatatgaagc ctccaaagcc aacaaaaatt
     1441 aaaaagaaag gtgtaaagaa gacattccag tgtgagcttt gcagttacac gtgtccacgg
     1501 cgttcaaatt tggatcgtca catgaaaagc cacactgatg agagaccaca caagtgccat
     1561 ctctgtggca gggcattcag aacagtcacc ctcctgagga atcaccttaa cacacacaca
     1621 ggtactcgtc ctcacaagtg cccagactgc gacatggcct ttgtgaccag tggagaattg
     1681 gttcggcatc gtcgttacaa acacacccac gagaagccat tcaagtgttc catgtgcgat
     1741 tacgccagtg tagaagtcag caaattaaaa cgtcacattc gctctcatac tggagagcgt
     1801 ccgtttcagt gcagtttgtg cagttatgcc agcagggaca catacaagct gaaaaggcac
     1861 atgagaaccc attcagggga aaagccttat gaatgttata tttgtcatgc tcggtttacc
     1921 caaagtggta ccatgaagat gcacatttta cagaagcaca cagaaaatgt ggccaaattt
     1981 cactgtcccc actgtgacac agtcatagcc cgaaaaagtg atttgggtgt ccacttgcga
     2041 aagcagcatt cctatattga gcaaggcaag aaatgccgtt actgtgatgc tgtgtttcat
     2101 gagcgctatg ccctcatcca gcatcagaag tcacacaaga atgagaagcg ctttaagtgt
     2161 gaccagtgtg attacgcttg tagacaggag aggcacatga tcatgcacaa gcgcacccac
     2221 accggggaga agccttacgc ctgcagccac tgcgataaga ccttccgcca gaagcagctt
     2281 ctcgacatgc acttcaagcg ctatcacgac cccaacttcg tccctgcggc ttttgtctgt
     2341 tctaagtgtg ggaaaacatt tacacgtcgg aataccatgg caagacatgc tgataattgt
     2401 gctggcccag atggcgtaga gggggaaaat ggaggagaaa cgaagaagag taaacgtgga
     2461 agaaaaagaa agatgcgctc taagaaagaa gattcctctg acagtgaaaa tgctgaacca
     2521 gatctggacg acaatgagga tgaggaggag cctgccgtag aaattgaacc tgagccagag
     2581 cctcagcctg tgaccccagc cccaccaccc gccaagaagc ggagaggacg accccctggc
     2641 agaaccaacc agcccaaaca gaaccagcca acagctatca ttcaggttga agaccagaat
     2701 acaggtgcaa ttgagaacat tatagttgaa gtaaaaaaag agccagatgc tgagcccgca
     2761 gagggagagg aagaggaggc ccagccagct gccacagatg cccccaacgg agacctcacg
     2821 cccgagatga tcctcagcat gatggaccgg ccggatccac cggtcatggt gagcaagggc
     2881 gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc tgaatggcga tgtgaatggc
     2941 cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3001 aagttcatct gcaccaccgg caagctgcct gtgccctggc ccaccctggt gaccaccctg
     3061 agctacggcg tgcagtgctt ctcacgctac cccgatcaca tgaagcagca cgacttcttc
     3121 aagagcgcca tgcctgaggg ctacatccag gagcgcacca tcttcttcga ggatgacggc
     3181 aactacaagt cgcgcgccga ggtgaagttc gagggcgata ccctggtgaa tcgcatcgag
     3241 ctgaccggca ccgatttcaa ggaggatggc aacatcctgg gcaataagat ggagtacaac
     3301 tacaacgccc acaatgtgta catcatgacc gacaaggcca agaatggcat caaggtgaac
     3361 ttcaagatcc gccacaacat cgaggatggc agcgtgcagc tggccgacca ctaccagcag
     3421 aataccccca tcggcgatgg ccctgtgctg ctgcccgata accactacct gtccacccag
     3481 agcgccctgt ccaaggaccc caacgagaag cgcgatcaca tgatctactt cggcttcgtg
     3541 accgccgccg ccatcaccca cggcatggat gagctgtaca agtgagcggc cgcgactcta
     3601 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3661 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3721 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3781 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     3841 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     3901 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     3961 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     4021 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     4081 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4141 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4201 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4261 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     4321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     4441 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4501 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     4561 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     4621 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4681 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4741 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatatg aaaaagcctg
     4801 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
     4861 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
     4921 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
     4981 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg
     5041 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg
     5101 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
     5161 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
     5221 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
     5281 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
     5341 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
     5401 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
     5461 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
     5521 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
     5581 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
     5641 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
     5701 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
     5761 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagttcta
     5821 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5881 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5941 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6001 tttttcactg cctcgcgatc cacaaccatg gcgaaagggc ctcgtgatac gcctattttt
     6061 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6121 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6181 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta ctgagagatc
     6241 ccctcataat ttccccaaag cgtaaccatg tgtgaataaa ttttgagcta gtagggttgc
     6301 agccacgagt aagtcttccc ttgttattgt gtagccagaa tgccgcaaaa cttccatgcc
     6361 taagcgaact gttgagagta cgtttcgatt tctgactgtg ttagcctgga agtgcttgtc
     6421 ccaaccttgt ttctgagcat gaacgcccgc aagccaacat gttagttgaa gcatcagggc
     6481 gattagcagc atgatatcaa aacgctctga gctgctcgtt cggctatggc gtaggcctag
     6541 tccgtaggca ggacttttca agtctcggaa ggtttcttca atctgcattc gcttcgaata
     6601 gatattaaca agttgtttgg gtgttcgaat ttcaacaggt aagttagttg ctagaaccca
     6661 tggctccttt gccgacgctg agtagatttt aggtgacggg tggtgacaat gagtccgtgt
     6721 cgagcgctga ttttttcggc ctttagagcg agatttatac aatagaattt ggcatgagat
     6781 tggattgctt ttagtcagcc tcttatagcc taaagtcttt gagtgactag atgacatatc
     6841 atgtaagttg ctgataggtt tccagttttc cgctcctagg tctgcatatt gtacttttcc
     6901 tcttactcga cttaaccagt accaacccag cttctcaacg gatttatacc atggcacttt
     6961 aaagccagca tcactgacaa tgagcggtgt ggtgttactc ggtagaatgc tcgcaaggtc
     7021 ggctagaaat tggtcatgag ctttctttga acattgctct gaaagcggga acgctttctc
     7081 ataaagagta acagaacgac cgtgtagtgc gactgaagct cgcaatacca taagtcgttt
     7141 ttgctcacga atatcagacc agtcaacaag tacaatgggc atcgtattgc ccgaacagat
     7201 aaagctagca tgccaacggt atacagcgag tcgctctttg tggaggtgac gattacctaa
     7261 caatcggtcg attcgtttga tgttatgttt tgttctcgct ttggttggca ggttacggcc
     7321 aagttcggta agagtgagag ttttacagtc aagtaatgcg tggcaagcca acgttaagct
     7381 gttgagtcgt tttaagtgta attcggggca gaattggtaa agagagtcgt gtaaaatatc
     7441 gagttcgcac atcttgttgt ctgattattg atttttcgcg aaaccatttg atcatatgac
     7501 aagatgtgta tccaccttaa cttaatgatt tttaccaaaa tcattagggg attcatcagg
     7561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7801 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8101 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8401 aggtgcctca ctgattaagc attggtaagg cgcgccaggc cctgccatag cctcaggtta
     8461 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8521 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8581 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8641 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8701 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8761 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8821 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8881 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8941 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9001 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9061 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9121 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9181 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9241 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9301 tattaccgcc atgcat
//
