LOCUS       sequence-267784-        7938 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mouse KIF3C 411-796.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF3C tail 411-796
FEATURES             Location/Qualifiers
     source          1..7938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        38..312
                     /label=chicken beta-actin promoter
     primer_bind     1255..1277
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1345..1347,1348..1350,1351..2058)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(1390..1411)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1651..1670)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1998..2019
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3394..3528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3431..3450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3485..3504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3559..3575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3559..3575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3572..3594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3583..3599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3607..3613,3614..3631,3632..3637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3652..3673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3790..3807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3961..4549)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4041..4060)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4720..5511,5512..5580))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5343..5362
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5581..5685)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5753..5771
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5809..5831)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5931..5950
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6144..6166
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6158..6175
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6159..6175
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggaggggcgc gtcacacccc cgccccacac cctcacctcg aggtgagccc cacgttctgc
       61 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
      121 ttattttgtg cagcgatggg ggcggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      181 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      241 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      301 gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc
      361 tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac
      421 ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg
      481 tggctgcgtg aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc
      541 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
      601 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
      661 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
      721 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta
      781 acccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
      841 tccgtgcggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
      901 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
      961 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1021 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1081 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1141 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc catctccagc
     1201 ctcggggctg tccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     1261 gcttctggcg tgtgaccggc ggggtttata tcttcccttc tctgttcctc cgcagccccc
     1321 aagcttggcg cgcctaggcc taccatggtg agcaagggcg gggagctgtt caccggggtg
     1381 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1441 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1501 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1561 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1621 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1681 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1741 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1801 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1861 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1921 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1981 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     2041 ggcatggacg agctgtacgg cggaggatcc ggaggcggct ctggcggagg gctgtacagg
     2101 agtcgtagaa agaaggccgt gtctgcccca gccggctatc cagaggggtc cgtgattgag
     2161 gcctgggtgg ccgaagagga agatgataac aacaacaatc accacccacc ccagcccatc
     2221 ttggaggcag ctttggagaa gaacatggag aattacctgc aggaccagaa ggaacggctg
     2281 gaggaggaga aggctgccat ccaggatgac cgcagccttg tgagtgagga gaagcagaag
     2341 cttctagaag agaaggagaa gatgctggag gatctacggc gggagcagca ggctacagag
     2401 ctgcttgcag ccaagtacaa ggccatggaa agcaagctac tcattggggg gcggaacatc
     2461 atggatcaca ccaacgagca acagaagatg ttggaactga agcgacagga gattgcggaa
     2521 cagaaacgcc gtgaacggga aatgcagcag gagatgctgc tccgcgatga agaaaccatg
     2581 gagctacgtg gtacctactc atccctgcag caggaggtgg aagttaaaac caagaaactc
     2641 aagaagctct atgccaagct acaggcggtg aaggccgaga tccaggacca acacgaagag
     2701 tacatccgcg tgcggcagga cctggaggag gcgcagaatg agcagacccg ggaactaaag
     2761 ctcaagtacc tcatcattga gaacttcatc ccccctgagg agaagaacaa gatcatgaac
     2821 cgccttttcc tggactgtga ggaggagcag tggaggttcc agccactggt gccagctgga
     2881 gtgaataaca gccaaatgaa gaagcgtcca acttctgcgg tgggctacaa gagacctatc
     2941 agccagtatg ctcgggttgc catggcaatg gggtcccatc ccagatacag agctgaaaat
     3001 ataatgtttt tggaactgga tgtgtcccca ccggctatct ttgagatgga gttttctcat
     3061 gaccaagagc aagacccacg ggtactacac atggagaggc tcatgaggtt ggacagcttt
     3121 ctggaaagac cgtccacaac taaagtgcga aagtcgagat cctggtgcca gagtcctcag
     3181 cggatgcctc caccctccac agctcatgca tccatgacct ctgttccttt gcaccctgca
     3241 acagtggtag accatgactg acggaccgtc taatctagat aagtaatgat cataatcagc
     3301 catatcacat ctgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3361 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3421 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3481 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccc cgggtaccga
     3541 gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4381 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5941 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     6001 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6061 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     6121 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     6181 gttgggatct ttgcattggc ccacggctct caggatgggg atgctcccct tcagcacccg
     6241 gttccccttg gaaactgatg gtcctggctc tgtggcatgg cagtggcact gtgaggagcc
     6301 ccctaccagc agcacacagt ggggttggca ctgccacgct ccggatgccg cgctctgatc
     6361 caaccccata atcaagggaa cccgaattgc cccatcattg cccccaccac ccccatcctg
     6421 ccgggccctc acaccccacg ctgccttctg gtgacattcc ccagcccaaa cccacggctt
     6481 catggctacc gcggggcatt tcccattgcc gccccattat cagctctgca cacctcccgc
     6541 tgtacccatg cctcgtggct gcccttcttt gacgtataat cttctaatta atacccggcc
     6601 ttgtcaaagt ggagcacaaa cgttaattaa ttccccagca ggcaggtaat taacagtgtg
     6661 actccctttt tgctgcgagt ggggctgata cagagagatg tggcactatg gagcccacgg
     6721 ggtcctggca ctgggtgccc acggaggtcc ccatgtgctg cagtgtcacc gcctccgagg
     6781 agacagtatt gtccctgcgg tgtccctgca gctcagctct gtccacaggg ccacctccag
     6841 tttggagggg acacaatgca gccccgatgc aacccatcct cgcagcatcc cagggacaaa
     6901 gaccccactg caagaccgca cacagggctg ggtcccgctc ccctaatatc tacagtgctt
     6961 cttgcatggc cccttaatca atgcagttaa tcagcatgcg ctcatgcacc gctctggagc
     7021 tgcaaagccc ctcgcagcgc tgctcaccaa caccgcgcac cgccccggcc cagcctgcag
     7081 cacgcgctgc aaacaggaaa gaaacaaaat attgcccaaa tgtaggcaaa ggcattcggc
     7141 tgccttgacc tccgccgggc cgggccctgc ctgactcagc tccttactca gcgctcgctt
     7201 cctccctccg gctgccaccg ccgcagcgca caccctgaca aagagtggcc cttaacgggc
     7261 tctgaggtgc acccagcagt gcactcagca gtccaagggc cggcctggag gtttgcaccg
     7321 ctacgtgctg acattagcat tgaacttggc cctgggtagt gctgcaggcc gggcggggtg
     7381 ggtgtagaga gtgcagcgcg cgttgcaccc ggtgcccctt cccctccctt gcatcccagc
     7441 aggctgcacc ccagcaccag gcccgtgcat gcatgctcct ggtgttattg cagcctggtg
     7501 catgcatgcg tcttgatggt gcagcgctgt gcatgcatcc tccttggtgt gtagcagtct
     7561 agtgcatgca tacccctcgg tgttattgct gctctgtgca cgcacgctca ttgtatcact
     7621 tcatcccagt gcatgcactc acactggagc gattgctgct cggtgcacgc acactcattg
     7681 tatcactgca gctcagtggc tgcacgcaca ccggtgttat tgctgctcgg tgcgtgcatg
     7741 cacatcagtg tcgctgcagc tcagtgcatg cacgctcatt gcccatcgct atccctgcct
     7801 ctcctgctgg cgctccccgg gaggtgactt caaggggacc gcaggaccac ctcgggggtg
     7861 gggggagggc tgcacacgcg gaccccgctc cccctcccca acaaagcact gtggaatcaa
     7921 aaagggggga ggggggat
//
